In Situ Hybridization In Light Microscopy PDF Download
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Author | : Gerard Morel |
Publisher | : CRC Press |
Total Pages | : 346 |
Release | : 2000-09-28 |
Genre | : Science |
ISBN | : 1420041827 |
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In Situ hybridization allows the visualization of specific DNA/RNA sequences in individual cells in tissue sections, single cells, or chromosome preparations, and is an especially important method for studying DNA and RNA in heterogeneous cell populations. This book delves into in situ hybridization methods through the use of light microscopy used
Author | : David G. Wilkinson |
Publisher | : OUP Oxford |
Total Pages | : 243 |
Release | : 1998-11-26 |
Genre | : Science |
ISBN | : 0191565709 |
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In situ hybridization is used to reveal the location of specific nucleic acids sequences on chromosomes or in tissues. Visualization of the location of genes on chromosomes or of specific mRNAs or viruses in tissues is crucial for understanding the organization, regulation, and function of genes. It is a therefore a core technique in all areas of biomedical research. In Situ Hybridization: A Practical Approach 2/e is the second edition of one of the most successful Practical Approach books, published in 1992. Since the first edition was published, a number of important technical advances have been made. The new edition has been thoroughly updated to contain protocols detailing the major techniques of in situ hybridization currently in use: in situ hybridization to mRNA with oligonucleotide and RNA probes (radiolabelled and hapten labelled); analysis using light and electron microscopes; whole mount in situ hybridization; double detection of RNAs, and RNA plus protein; and fluorescent in situ hybridization to detect chromosomal sequences. The protocols are complemented by advice on strategies for successful results, descriptions of the theoretical basis of in situ hybridization and important new developments in gene expression databases. The procedures described are widely applicable to many systems. The use of in situ hybridization in PCR is covered in a separate volume: Herrington and O'Leary (Eds) PCR 3 - PCR in situ hybridization: A Practical Approach (OUP, 1997). All the authors have extensive practical experience of establishing reliable techniques of in situ hybridization. This book will be useful to all researchers at all levels who use in situ hybridization.
Author | : Thomas Liehr |
Publisher | : Springer |
Total Pages | : 0 |
Release | : 2010-10-15 |
Genre | : Science |
ISBN | : 9783642089527 |
Download Fluorescence In Situ Hybridization (FISH) - Application Guide Book in PDF, ePub and Kindle
This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.
Author | : Gal Haimovich |
Publisher | : Springer Nature |
Total Pages | : 303 |
Release | : |
Genre | : |
ISBN | : 1071637665 |
Download Fluorescence In Situ Hybridization (FISH) Book in PDF, ePub and Kindle
Author | : Julian E. Beesley |
Publisher | : Springer Science & Business Media |
Total Pages | : 277 |
Release | : 2012-12-06 |
Genre | : Science |
ISBN | : 146120139X |
Download Immunocytochemistry and In Situ Hybridization in the Biomedical Sciences Book in PDF, ePub and Kindle
Immunocytochemistry and in situ hybridization are widely used biomedical sciences. They are essential in medical diagnosis and in cell biology research. Affinity labeling is the central goal of the experimental strategy involving a series of techniques in a logical order; from the effects of specimen fixation, through specimen preparation to expose the antigen, to optimizing immunolabeling, to assessing the result and finally to safety considerations. Numerous examples of these techniques in biomedical sciences are included, as well as experimental assays and practical tips. This survey of methods will serve as an invaluable reference source in any laboratory setting (academic, industrial or clinical) involved in research in almost every branch of biology or medicine, as well as in pharmaceutical, biotechnological and clinical applications.
Author | : G.R. Coulton |
Publisher | : Springer Science & Business Media |
Total Pages | : 126 |
Release | : 2012-12-06 |
Genre | : Medical |
ISBN | : 9401129843 |
Download In Situ Hybridization: Medical Applications Book in PDF, ePub and Kindle
In situ hybridization has developed as a means of localizing specific DNA and RNA sequences within tissues. The great strength of this approach is the ability to relate the distribution of specific nucleic acids with cell structures and the protein products of the target gene by means of immunohistochemistry. Complementary DNA, RNA or oligonucleotide probes, suitably labelled, are hybridized to specific DNA or RNA targets within tissues. The spatial information thus obtained has contributed greatly to our understanding of such diverse areas of research as gene mapping, viral infection, cytogenetics, protein synthesis, prenatal diagnosis and tissue grafting. This book is not intended as another recipe book, although it does describe theoretical and practical aspects of the technology. Rather, the authors critically describe the contribution made by in situ hybridization to specific areas of medical research.
Author | : LEITCH |
Publisher | : Springer |
Total Pages | : 132 |
Release | : 1994-06-30 |
Genre | : Science |
ISBN | : |
Download SITU HYBRIDIZATION Book in PDF, ePub and Kindle
This practical guide provides a comprehensive description of in situ hybridization, from background information to detailed and practical applications. In situ hybridization is a powerful link between cellular and molecular biology, and can be used to localize nucleic acid sequences.
Author | : Oncor Inc |
Publisher | : |
Total Pages | : |
Release | : 1995 |
Genre | : |
ISBN | : |
Download Chromosome In Situ Hybridization Manual Book in PDF, ePub and Kindle
Author | : Nancy Jean Hutchison |
Publisher | : |
Total Pages | : 328 |
Release | : 1982 |
Genre | : Electron microscopy |
ISBN | : |
Download In Situ Hybridization at the Electron Microscope Level Book in PDF, ePub and Kindle
Author | : Melody Clark |
Publisher | : |
Total Pages | : 194 |
Release | : 1996 |
Genre | : Science |
ISBN | : |
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