Identification And Characterization Of Cold Tolerance Associated Genes In Wheat PDF Download
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Author | : Muhammad Zayed |
Publisher | : |
Total Pages | : |
Release | : 2019 |
Genre | : |
ISBN | : |
Download Identification and Characterization of Cold-Tolerance Associated Genes in Wheat Book in PDF, ePub and Kindle
The low temperature remains as one of the major limiting factors of crop productivity in the temperate region, and identification of cold tolerance related genes is crucial for developing cold tolerant crop plants to increase agricultural productivity. The objective of my thesis is to identify cold tolerance related candidate genes in wheat, one of the major crops in the temperate region. In Chapter 2, I have reviewed the literature pertaining to the mechanisms of cold tolerance in plants with specific emphasis on Wheat. In Chapter 3, forty candidate genes with increased expression under cold exposure based on published microarray data were selected and further characterized. These genes belonging to four categories namely defense-related regulators; transcriptional and epigenetic regulators; post-transcriptional and post-translational regulators; and genes of unknown functions revealed many differentially expressed genes including Remorin - upregulated in response to cold; a novel gene in wheat homologous to RD29B of Arabidopsis-upregulated in response to cold and ABA; and another novel gene regulated by both ABA and MetJA. In chapter 4, the results of genome-wide identification and characterization of the wheat remorin family and its association with cold tolerance are presented. A search of the wheat database revealed the existence of twenty different remorin genes that we classified into six groups sharing a common structure and phylogenetic origin. Promoter analysis of TaREM genes revealed the presence of putative cis-elements related to diverse functions like development, hormonal regulation, biotic and abiotic stress responsiveness. Expression levels of TaREM genes were measured in plants grown under in field and laboratory conditions and in response to hormone treatment. Our analyses revealed twelve members of the remorin family that are regulated during cold acclimation of wheat in four different tissues (root, crown, stem and leaves), with the highest expression in roots. Differential gene expression was found between wheat cultivars with contrasting degree of cold tolerance suggesting the implication of TaREM genes in cold response and tolerance. Additionally, eight genes were induced in response to ABA and MetJA treatment. This genome-wide analysis of TaREM genes provides valuable resources for functional analysis aimed at understanding their role in stress adaptation. The chapter 5 is focused on gaining insights into the evolutionary history and in-silico functional characterization of a novel cold-responsive gene in wheat. This gene in wheat has distant homology to known abiotic stress-related genes in other plants including CAP160 in Spinacia oleracea, RD29B in Arabidopsis and CDeT11-24 in Craterostigma plantagineum. The results show that these genes are homologous and may have evolved from a common ancestor. The Bayesian phylogenetic analyses of the protein sequences of this gene from various plant species revealed three distinctive clades. Further analyses revealed that this gene has predominantly evolved through neutral processes with some regions experiencing signatures of negative selections and some regions showing signatures of episodic positive selections. These genes contained common K-like segments and function predictions revealed that these protein-coding genes may share at least two functions related to abiotic stress conditions. One function is similar to the cryoprotective function of LEA protein, and the second function as a signalling molecule by binding specifically to phosphatidic acid.
Author | : Raul Antonio Sperotto |
Publisher | : Frontiers Media SA |
Total Pages | : 739 |
Release | : 2022-02-24 |
Genre | : Science |
ISBN | : 2889740099 |
Download Identification and Characterization of Contrasting Genotypes/Cultivars to Discover Novel Players in Crop Responses to Abiotic/Biotic Stresses Book in PDF, ePub and Kindle
Author | : Shabir Hussain Wani |
Publisher | : Springer |
Total Pages | : 203 |
Release | : 2018-11-24 |
Genre | : Science |
ISBN | : 3030014150 |
Download Cold Tolerance in Plants Book in PDF, ePub and Kindle
Cold stress is one of the prevalent environmental stresses affecting crop productivity, particularly in temperate regions. Numerous plant types of tropical or subtropical origin are injured or killed by non-freezing low temperature, and display a range of symptoms of chilling injury such as chlorosis, necrosis, or growth retardation. In contrast, chilling tolerant species thrive well at such temperatures. To thrive under cold stress conditions, plants have evolved complex mechanisms to identify peripheral signals that allow them to counter varying environmental conditions. These mechanisms include stress perception, signal transduction, transcriptional activation of stress-responsive target genes, and synthesis of stress-related proteins and other molecules, which help plants to strive through adverse environmental conditions. Conventional breeding methods have met with limited success in improving the cold tolerance of important crop plants through inter-specific or inter-generic hybridization. A better understanding of physiological, biochemical and molecular responses and tolerance mechanisms, and discovery of novel stress-responsive pathways and genes may contribute to efficient engineering strategies that enhance cold stress tolerance. It is therefore imperative to accelerate the efforts to unravel the biochemical, physiological and molecular mechanisms underlying cold stress tolerance in plants. Through this new book, we intend to integrate the contributions from plant scientists targeting cold stress tolerance mechanisms using physiological, biochemical, molecular, structural and systems biology approaches. It is hoped that this collection will serve as a reference source for those who are interested in or are actively engaged in cold stress research.
Author | : Ramanjulu Sunkar |
Publisher | : Springer Nature |
Total Pages | : 293 |
Release | : |
Genre | : |
ISBN | : 1071639730 |
Download Plant Stress Tolerance Book in PDF, ePub and Kindle
Author | : Tyrel Jonathan Denesik |
Publisher | : |
Total Pages | : |
Release | : 2007 |
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Download Quantitative Expression Analysis of Four Low-Temperature-Tolerance-Associated Genes During Cold Acclimation in Wheat ('Triticum Aestivum 'L.). Book in PDF, ePub and Kindle
Author | : Nikita |
Publisher | : |
Total Pages | : 0 |
Release | : 2023 |
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Download Identification and Characterization of Meta- QTLs,ortho-MQTLs, and Candidate Genes for the Traits Associated with Lodging Tolerance in Common Wheat (Triticum Aestivum L.).[ With CD Copy ] Book in PDF, ePub and Kindle
Author | : Jauhar Ali |
Publisher | : Springer Nature |
Total Pages | : 507 |
Release | : 2021-05-05 |
Genre | : Technology & Engineering |
ISBN | : 3030665305 |
Download Rice Improvement Book in PDF, ePub and Kindle
This book is open access under a CC BY 4.0 license. By 2050, human population is expected to reach 9.7 billion. The demand for increased food production needs to be met from ever reducing resources of land, water and other environmental constraints. Rice remains the staple food source for a majority of the global populations, but especially in Asia where ninety percent of rice is grown and consumed. Climate change continues to impose abiotic and biotic stresses that curtail rice quality and yields. Researchers have been challenged to provide innovative solutions to maintain, or even increase, rice production. Amongst them, the ‘green super rice’ breeding strategy has been successful for leading the development and release of multiple abiotic and biotic stress tolerant rice varieties. Recent advances in plant molecular biology and biotechnologies have led to the identification of stress responsive genes and signaling pathways, which open up new paradigms to augment rice productivity. Accordingly, transcription factors, protein kinases and enzymes for generating protective metabolites and proteins all contribute to an intricate network of events that guard and maintain cellular integrity. In addition, various quantitative trait loci associated with elevated stress tolerance have been cloned, resulting in the detection of novel genes for biotic and abiotic stress resistance. Mechanistic understanding of the genetic basis of traits, such as N and P use, is allowing rice researchers to engineer nutrient-efficient rice varieties, which would result in higher yields with lower inputs. Likewise, the research in micronutrients biosynthesis opens doors to genetic engineering of metabolic pathways to enhance micronutrients production. With third generation sequencing techniques on the horizon, exciting progress can be expected to vastly improve molecular markers for gene-trait associations forecast with increasing accuracy. This book emphasizes on the areas of rice science that attempt to overcome the foremost limitations in rice production. Our intention is to highlight research advances in the fields of physiology, molecular breeding and genetics, with a special focus on increasing productivity, improving biotic and abiotic stress tolerance and nutritional quality of rice.
Author | : |
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Release | : 2004 |
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Download Quantitative Expression Analysis of Four Low-temperature-tolerance-associated Genes During Cold Acclimation in Wheat ([i]Triticum Aestivum [/i]L.). Book in PDF, ePub and Kindle
Author | : Simon Drouin |
Publisher | : |
Total Pages | : |
Release | : 2004 |
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ISBN | : |
Download Gene Expression Comparison by Microarray Analysis Between Two Triticum Aestivum Cultivars that Differ in Freezing Tolerance Book in PDF, ePub and Kindle
Author | : K.V. Madhava Rao |
Publisher | : Springer Science & Business Media |
Total Pages | : 372 |
Release | : 2006-02-10 |
Genre | : Science |
ISBN | : 9781402042249 |
Download Physiology and Molecular Biology of Stress Tolerance in Plants Book in PDF, ePub and Kindle
Biologists worldwide now speak the scientific language of molecular biology and use the same molecular tools. Interest is growing in the molecular biology of abiotic stress tolerance and modes of installing better tolerant mechanisms in crop plants. Current studies make plants capable of sustaining their yields even under stressful conditions. Further, this information may form the basis for its application in biotechnology and bioinformatics.