Hydrophilic Interaction Liquid Chromatography Hilic And Advanced Applications PDF Download
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| Author | : Perry G. Wang |
| Publisher | : CRC Press |
| Total Pages | : 599 |
| Release | : 2011-02-17 |
| Genre | : Medical |
| ISBN | : 143980754X |
Download Hydrophilic Interaction Liquid Chromatography (HILIC) and Advanced Applications Book in PDF, ePub and Kindle
This is the first book that comprehensively and systematically describes the new technology of hydrophilic interaction liquid chromatography (HILIC). Hydrophilic interaction chromatography is a separation technique suitable for polar and hydrophilic compounds and orthogonal to reversed phase liquid chromatography. From small organic molecules to pr
| Author | : Perry G. Wang |
| Publisher | : CRC Press |
| Total Pages | : 613 |
| Release | : 2011-02-17 |
| Genre | : Medical |
| ISBN | : 1439807531 |
Download Hydrophilic Interaction Liquid Chromatography (HILIC) and Advanced Applications Book in PDF, ePub and Kindle
This is the first book that comprehensively and systematically describes the new technology of hydrophilic interaction liquid chromatography (HILIC). Hydrophilic interaction chromatography is a separation technique suitable for polar and hydrophilic compounds and orthogonal to reversed phase liquid chromatography. From small organic molecules to proteins, the text explores the many applications of HILIC in the analytical field. Winner of the President's Award for Excellence, the author explains how HILIC can significantly improve analytical throughput by shortening sample preparation procedure, which is one of the bottlenecks for drug discovery and development in the pharmaceutical industry.
| Author | : A. Cavazzini |
| Publisher | : Elsevier Inc. Chapters |
| Total Pages | : 26 |
| Release | : 2013-01-08 |
| Genre | : Science |
| ISBN | : 012807132X |
Download Liquid Chromatography Book in PDF, ePub and Kindle
Hydrophilic interaction liquid chromatography (HILIC) has become in the last years a leading technique for the separation of small and large polar molecules. This chapter begins with a discussion about the most relevant characteristics of HILIC mode that makes it a complementary and, in some cases, alternative technique to the more traditional reversed- and normal-phase modes. Subsequently, the fundamentals of retention mechanisms in HILIC are revised in light of the most recent findings in the field. The chapter ends with an overview of the materials used as stationary and mobile phases in HILIC and of the applications of this analytical technique in different branches of science.
| Author | : Bernard A. Olsen |
| Publisher | : John Wiley & Sons |
| Total Pages | : 336 |
| Release | : 2013-01-22 |
| Genre | : Science |
| ISBN | : 1118054172 |
Download Hydrophilic Interaction Chromatography Book in PDF, ePub and Kindle
Discover how to use HILIC to analyze and better understand polar compounds An increasingly popular analytical method, hydrophilic interaction chromatography (HILIC) has the ability to retain and separate polar compounds that are often difficult to analyze by reversed-phase high-performance liquid chromatography (HPLC) or other analytical methods. Offering a comprehensive review, this book enables readers to develop a fundamental understanding of how HILIC works and then apply that knowledge to develop and implement a variety of practical applications. Hydrophilic Interaction Chromatography begins with discussions of HILIC retention mechanisms, stationary phases, and general method development. This sets the foundation for the book's extensive coverage of applications. The authors address unique separation challenges for bioanalytical, environmental, pharmaceutical, and biochemical applications. Moreover, there is a thorough discussion of HILIC in two-dimensional chromatography. With contributions from leading analytical scientists who have extensive experience in HILIC as well as HPLC, Hydrophilic Interaction Chromatography serves as a practical guide for researchers, featuring: Detailed examples of HILIC methods and development approaches Thorough explanations of retention mechanisms and the impact of stationary phase and mobile phase properties on separations Step-by-step guidance for developing efficient, sensitive, and robust HILIC methods References to the primary literature at the end of each chapter Hydrophilic Interaction Chromatography is written for scientists who use or develop analytical methods for the separation of polar compounds. In particular, these researchers will discover how HILIC can be used to analyze and better understand the composition of pharmaceutical, bioanalytical, biochemical, chemical, food, and environmental samples.
| Author | : Michal Holcapek |
| Publisher | : Elsevier |
| Total Pages | : 522 |
| Release | : 2017-09-07 |
| Genre | : Technology & Engineering |
| ISBN | : 0128117338 |
Download Handbook of Advanced Chromatography /Mass Spectrometry Techniques Book in PDF, ePub and Kindle
Handbook of Advanced Chromatography /Mass Spectrometry Techniques is a compendium of new and advanced analytical techniques that have been developed in recent years for analysis of all types of molecules in a variety of complex matrices, from foods to fuel to pharmaceuticals and more. Focusing on areas that are becoming widely used or growing rapidly, this is a comprehensive volume that describes both theoretical and practical aspects of advanced methods for analysis. Written by authors who have published the foundational works in the field, the chapters have an emphasis on lipids, but reach a broader audience by including advanced analytical techniques applied to a variety of fields. Handbook of Advanced Chromatography / Mass Spectrometry Techniques is the ideal reference for those just entering the analytical fields covered, but also for those experienced analysts who want a combination of an overview of the techniques plus specific and pragmatic details not often covered in journal reports. The authors provide, in one source, a synthesis of knowledge that is scattered across a multitude of literature articles. The combination of pragmatic hints and tips with theoretical concepts and demonstrated applications provides both breadth and depth to produce a valuable and enduring reference manual. It is well suited for advanced analytical instrumentation students as well as for analysts seeking additional knowledge or a deeper understanding of familiar techniques. Includes UHPLC, HILIC, nano-liquid chromatographic separations, two-dimensional LC-MS (LCxLC), multiple parallel MS, 2D-GC (GCxGC) methodologies for lipids analysis, and more Contains both practical and theoretical knowledge, providing core understanding for implementing modern chromatographic and mass spectrometric techniques Presents chapters on the most popular and fastest-growing new techniques being implemented in diverse areas of research
| Author | : Stavros Kromidas |
| Publisher | : John Wiley & Sons |
| Total Pages | : 248 |
| Release | : 2019-04-04 |
| Genre | : Science |
| ISBN | : 3527812776 |
Download Gradient HPLC for Practitioners Book in PDF, ePub and Kindle
This practical guide for analytical scientists explains the use of gradients in liquid chromatography. The fundamentals of gradient separations, as well as the most common application scenarios are addressed, from LC-MS coupling to biochromatography to the separation of ionic substances. Throughout, this handy volume provides detailed hands-on information for practitioners, enabling them to use gradient separation methods reliably and efficiently.
| Author | : Muhammad Anas Kamleh |
| Publisher | : |
| Total Pages | : |
| Release | : 2010 |
| Genre | : |
| ISBN | : |
Download The Combination of Hydrophilic Interaction Liquid Chromatography and High Resolution Mass Spectrometry (HILIC-HRMS) Book in PDF, ePub and Kindle
| Author | : Gregory K. Webster |
| Publisher | : CRC Press |
| Total Pages | : 412 |
| Release | : 2014-02-04 |
| Genre | : Medical |
| ISBN | : 9814463019 |
Download Supercritical Fluid Chromatography Book in PDF, ePub and Kindle
Analytical chemists in the pharmaceutical industry are always looking for more-efficient techniques to meet the analytical challenges of today's pharmaceutical industry. One technique that has made steady advances in pharmaceutical analysis is supercritical fluid chromatography (SFC). SFC is meeting the chromatography needs of the industry by provi
| Author | : Yuegang Zuo |
| Publisher | : Nova Science Publishers |
| Total Pages | : 0 |
| Release | : 2014 |
| Genre | : Chromatographic analysis |
| ISBN | : 9781629488547 |
Download High-performance Liquid Chromatography (HPLC) Book in PDF, ePub and Kindle
Includes bibliographical references and index.
| Author | : Ya Zhang |
| Publisher | : |
| Total Pages | : 125 |
| Release | : 2014 |
| Genre | : Hydrophilic interaction liquid chromatography |
| ISBN | : |
Download Liquid Chromatography Book in PDF, ePub and Kindle
Ion Chromatography (IC) is a powerful technique in the separation and analysis of inorganic ions and small charged organic molecules. Meanwhile, Hydrophilic Interaction Liquid Chromatography (HILIC) enables the separation of polar hydrophilic compounds. Although their retention mechanisms are different, the same goal applies. Chromatographers want a fast and thorough separation. This thesis focuses on improving separations in both modes. Injection solvent mismatch in Reversed Phase Liquid Chromatography (RPLC) leads to peak broadening and even distortion. In this thesis, systematic studies on IC columns showed that the characteristics of injection solvent mismatch broadening are very different than in RPLC. Also, IC is much more tolerant to high matrix concentration. The sensitivity parameter for evaluating RPLC injection solvent response was applied in the IC column studied for the comparison and can be further used in evaluating other IC columns. In modern IC, suppressors are widely used. Injection system peaks are usually neglected because they are eliminated by the suppressor. However this thesis showed that the system peak strongly affects the nearby analyte peak shape and retention. Porous Graphitic Carbon (PGC) is popular in some RPLC applications due to its excellent pH and temperature stability. This stability would also be attractive for HILIC applications. However, the hydrophobic character of PGC makes it incompatible with HILIC separations. In this thesis, PGC was converted into a hydrophilic phase by attaching acetanilide to the surface. The new Amide-PGC shows unique selectivity among 37 stationary phases under HILIC mode. The thesis demonstrates its potential in separating nucleobases, carboxylic acids and pharmaceuticals. Retention is shown to be due to both HILIC partitioning and adsorption on the PGC surface. In summary, this thesis improves liquid chromatography in two aspects: IC separations through an understanding of injection broadening; and HILIC through the development of a new stationary phase which enables unique HILIC selectivity.
