Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Histology Immunohistochemistry And In Situ Hybridisation Lab Protocols PDF full book. Access full book title Histology Immunohistochemistry And In Situ Hybridisation Lab Protocols.
| Author | : Jenny Steel |
| Publisher | : Lulu.com |
| Total Pages | : 56 |
| Release | : 2017-07-04 |
| Genre | : Science |
| ISBN | : 1326988158 |
This book is designed to be a manual for laboratory use, based upon the author's own experience and successful published results. It is for the use of students and researchers.
| Author | : Gerard J. Nuovo |
| Publisher | : Academic Press |
| Total Pages | : 418 |
| Release | : 2020-09-03 |
| Genre | : Science |
| ISBN | : 0128206543 |
Major advancements in the field of in situ molecular pathology have occurred since publication of the first edition. In Situ Molecular Pathology and Co-expression Analyses, Second Edition, continues to teach both the molecular basis for the improvements and the actual protocols. This is the unique feature that separates it from the pack of other "cook-book" type approaches. The fields of in situ hybridization and immunohistochemistry have expanded rapidly where computer-based analyses systems have greatly expanded the power of these methods. Further, knowledge of the marked improvements in the reagents themselves since the first edition can make the difference of excellent versus misleading data. The automated platforms require that researchers and diagnostic biomedical investigators have a good understanding of the basics of in situ based tests, protocols, and biochemistry for troubleshooting in order to maximize the use of these platforms. This second edition focuses attention on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses. Practicing molecular pathologists, diagnostic pathologists, laboratory directors, and toxicologists, as well as clinicians and researchers in training, will benefit from this clear presentation of protocols and theoretical framework. Data derived from in situ hybridization and immunohistochemistry. Explains the theory and foundation of immunohistochemistry and in situ hybridization and presents easy-to-follow experimental protocols with tricks of the trade Includes two new chapters: Recent improvements in immunohistochemistry and in situ hybridization, Quality control for immunohistochemistry and in situ hybridization: How to know if the color change is signal or background The second edition also includes a detailed test to help one learn the basics of histologic interpretation of tissues and a separate detailed test in how to differentiate signal from background Includes chapter-ending summaries of Key Points to Remember, bringing beginners up to speed with any seasoned veteran in the field Thoughtfully tackles the molecular basis if IHC and ISH, along with application of that knowledge to improving the techniques is significant
| Author | : M.M. Bui |
| Publisher | : Karger Medical and Scientific Publishers |
| Total Pages | : 120 |
| Release | : 2020-01-13 |
| Genre | : Medical |
| ISBN | : 3318065765 |
In the era of precision medicine, physicians are increasingly in need of more definitive diagnostic, prognostic, and predictive information derived from small biopsy specimens such as cytology samples in order to guide effective patient care. Cytopathology is well poised to meet this challenge. Whilst the traditional cytomorphologic component of cytology practice is still valid, enormous advances have been made in the field of cytopathology thanks to transformative technology and innovative individuals that have augmented the cytologists' ability to meet the demands of modern medicine. The purpose of this book is to describe, illustrate, and review many of the most recent developments regarding modern techniques employed in cytopathology. This latest monograph is intended for all cytologists including cytopathologists, cytotechnologists, cytology lab assistants, trainees, research scientists, and anyone who is interested in the field of cytopathology. We have invited pioneering experts in their respective fields to author these chapters. This book is not only the culmination of their groundbreaking work and effort but also presents a critical review of the current literature. We have attempted to provide readers with an informative and comprehensive aid so that they may better appreciate how emerging technology has been applied to cytology. Each chapter in this book presents a stand-alone contemporary review of emerging topics in cytopathology. We hope that you will find this monograph thought-provoking and a valuable reference for your practice.
| Author | : Andrew J Connolly |
| Publisher | : Elsevier Health Sciences |
| Total Pages | : 403 |
| Release | : 2015-09-23 |
| Genre | : Medical |
| ISBN | : 0323287808 |
A mainstay for pathology residents, Autopsy Pathology is designed with a uniquely combined manual and atlas format that presents today's most complete coverage of performing, interpreting, and reporting post-mortem examinations. This lasting and useful medical reference book offers a practical, step-by-step approach to discussing not only the basics of the specialty, but the performance of specialized autopsy procedures as well. Material is divided into two sections for ease of use: a manual covering specific autopsy procedures, biosafety, generation of autopsy reports, preparation of death certificates, and other essential subjects; and an atlas, organized by organ system, which captures the appearance of the complete spectrum of autopsy findings. Offers expanded coverage of microscopic anatomy. Includes a chapter on performing special dissection procedures that may not be covered during a typical residency. Examines important techniques, such as autopsy photography and radiology, microscopic examination, supplemental laboratory studies, and other investigative approaches. Addresses the latest legal, social, and ethical issues relating to autopsies, as well as quality improvement and assurance. Presents more than 600 full-color photographs depicting common gross and microscopic autopsy findings for every part of the body. Correlates pathologic findings with their clinical causes to enhance diagnostic accuracy. Improved images in the Atlas section provide greater visual understanding. Additional online features include dissection videos demonstrating autopsy techniques; downloadable, commonly used forms for autopsy reports; and calculators for weights and measures. Expert Consult eBook version included with purchase. This enhanced eBook experience offers access to all of the text, figures, images, videos, forms, calculators, and references from the book on a variety of devices.
| Author | : Walter E. Finkbeiner |
| Publisher | : Elsevier Health Sciences |
| Total Pages | : 377 |
| Release | : 2009-02-17 |
| Genre | : Medical |
| ISBN | : 1437719716 |
This how-to guide presents today's most complete coverage of performing, interpreting, and reporting post-mortem examinations. In addition to discussing the basics of the specialty, this lasting and useful reference features information on the performance of specialized autopsy procedures. The material is divided into two sections for ease of use: a manual covering specific autopsy procedures, biosafety, generation of autopsy reports, preparation of death certificates, and other essential subjects; and an atlas, organized by organ system, that captures the appearance of the complete spectrum of autopsy findings. The updated second edition features a new chapter on the popular topic of forensic pathology. Focuses on hospital autopsy, while also providing a brief introduction to forensic autopsy. Examines autopsy photography and radiology, microscopic examination, supplemental laboratory studies, and other investigative approaches. Includes a chapter on performing special dissection procedures that are usually not covered during a typical residency. Presents over 590 full-color photographs depicting common gross and microscopic autopsy findings for every part of the body. Correlates pathologic findings with their clinical causes to enhance diagnostic accuracy. Covers the hot topic of forensic pathology in a new chapter introducing the subspecialty. Addresses the latest legal, social, and ethical issues as well as quality improvement and quality assurance. Features improved images in the Atlas section to give an even more useful visual reference.
| Author | : Bernd W. Rautenstrauß |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 493 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 3642564046 |
Fluorescence in situ hybridization (FISH) has been developed as a powerful technology which allows direct visualisation or localisation of genomic alterations. The technique has been adopted to a range of applications in both medicine, especially in the areas of diagnostic cytogenetics, and biology. Topics described in this manual include: FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells; FISH on archival human tissues, such as formalin fixed and paraffin embedded tissue sections, cryofixed tissue; simultaneous detection of apoptosis and xpression of apoptosis-related genes; comparative genomic ybridization; and special FISH techniques.
| Author | : Simon Renshaw |
| Publisher | : John Wiley & Sons |
| Total Pages | : 258 |
| Release | : 2017-02-06 |
| Genre | : Science |
| ISBN | : 1118717775 |
Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount. It is with this fundamental aim in mind that we have compiled Immunohistochemistry: Essential Methods. We have achieved this by examining each aspect of immunochemistry in turn, with each chapter including detailed information regarding the subject matter in question. Each chapter is written by an expert in their field and includes protocols that are typically used in their own research. Subjects covered are, amongst others, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy. In addition, benefits and limitations of each approach are discussed within the chapters.
| Author | : David J. Dabbs |
| Publisher | : Churchill Livingstone |
| Total Pages | : 858 |
| Release | : 2006 |
| Genre | : Medical |
| ISBN | : |
Offers current information and guidance on immunohistochemical diagnoses in surgical pathology and cytopathology. The book is organized in a consistent format of organ systems, specifically designed to target diagnostic dilemmas in surgical pathology. Each chapter features an introduction and a systematic approach to the diagnostic entities of that organ system. Provides a reference for pathologists practicing diagnostic surgical pathology and cytopathology. Structured in the organ-system approach to tumor pathology, with each chapter capable of standing alone. Extensive references and full-color illustrations are included.
| Author | : Shan-Rong Shi |
| Publisher | : Eaton Publishing Company/Biotechniques Books |
| Total Pages | : 388 |
| Release | : 2000 |
| Genre | : Medical |
| ISBN | : |
| Author | : Mai P. Hoang |
| Publisher | : Cambridge University Press |
| Total Pages | : 315 |
| Release | : 2017-09-28 |
| Genre | : Medical |
| ISBN | : 1107150167 |
One of only a few textbooks on the market dedicated to the important role of immunohistochemistry in diagnostic dermatopathology.
