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Handbook of Biological Confocal Microscopy

Handbook of Biological Confocal Microscopy
Author: James Pawley
Publisher: Springer Science & Business Media
Total Pages: 639
Release: 2013-04-17
Genre: Science
ISBN: 1475753489

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This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.


Handbook of Biological Confocal Microscopy

Handbook of Biological Confocal Microscopy
Author: James Pawley
Publisher: Springer Science & Business Media
Total Pages: 234
Release: 2012-12-06
Genre: Medical
ISBN: 1461571332

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In 1987 the Electron Microscopy Society of America (EMSA) going to drive important scientific discoveries across wide areas under the leadership of J. P. Revel (Cal Tech) initiated a major of physiology, cellular biology and neurobiology. They had been program to present a discussion of recent advances in light looking for a forum in which they could advance the state of microscopy as part of the annual meeting. The result was three the art of confocal microscopy, alert manufacturers to the lim special LM sessions at the Milwaukee meeting in August 1988: itations of current instruments, and catalyze progress toward The LM Forum, organized by me, and Symposia on Confocal new directions in confocal instrument development. LM, organized by G. Schatten (Madison), and on Integrated These goals were so close to those of the EMSA project that Acoustic/LM/EM organized by C. Rieder (Albany). In addition, the two groups decided to join forces with EMSA to provide there was an optical micro-analysis session emphasizing Raman the organization and the venue for a Confocal Workshop and techniques, organized by the Microbeam Analysis Society, for NSF to provide the financial support for the speakers expenses a total of 40 invited and 30 contributed papers on optical tech and for the publication of extended abstracts.


Handbook of Biological Confocal Microscopy

Handbook of Biological Confocal Microscopy
Author: James B. Pawley
Publisher: Springer Science & Business Media
Total Pages: 672
Release: 1995
Genre: Medical
ISBN: 9780306448263

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This newly updated second edition details the latest instrumentation and applications of the confocal microscope. This edition features 21 new chapters and includes information on preparing living specimens for the confocal microscope.


Handbook of Biological Confocal Microscopy

Handbook of Biological Confocal Microscopy
Author: James Pawley
Publisher: Springer Science & Business Media
Total Pages: 1014
Release: 2010-08-04
Genre: Science
ISBN: 0387455248

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Once the second edition was safely off to the printer, the 110 larger world of micro-CT and micro-MRI and the smaller world authors breathed a sigh of relief and relaxed, secure in the belief revealed by the scanning and transmission electron microscopes. that they would “never have to do that again. ” That lasted for 10 To round out the story we even have a chapter on what PowerPoint years. When we ?nally awoke, it seemed that a lot had happened. does to the results, and the annotated bibliography has been In particular, people were trying to use the Handbook as a text- updated and extended. book even though it lacked the practical chapters needed. There As with the previous editions, the editor enjoyed a tremendous had been tremendous progress in lasers and ?ber-optics and in our amount of good will and cooperation from the 124 authors understanding of the mechanisms underlying photobleaching and involved. Both I, and the light microscopy community in general, phototoxicity. It was time for a new book. I contacted “the usual owe them all a great debt of gratitude. On a more personal note, I suspects” and almost all agreed as long as the deadline was still a would like to thank Kathy Lyons and her associates at Springer for year away.


Data Processing Handbook for Complex Biological Data Sources

Data Processing Handbook for Complex Biological Data Sources
Author: Gauri Misra
Publisher: Academic Press
Total Pages: 188
Release: 2019-03-23
Genre: Medical
ISBN: 0128172800

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Data Processing Handbook for Complex Biological Data provides relevant and to the point content for those who need to understand the different types of biological data and the techniques to process and interpret them. The book includes feedback the editor received from students studying at both undergraduate and graduate levels, and from her peers. In order to succeed in data processing for biological data sources, it is necessary to master the type of data and general methods and tools for modern data processing. For instance, many labs follow the path of interdisciplinary studies and get their data validated by several methods. Researchers at those labs may not perform all the techniques themselves, but either in collaboration or through outsourcing, they make use of a range of them, because, in the absence of cross validation using different techniques, the chances for acceptance of an article for publication in high profile journals is weakened. Explains how to interpret enormous amounts of data generated using several experimental approaches in simple terms, thus relating biology and physics at the atomic level Presents sample data files and explains the usage of equations and web servers cited in research articles to extract useful information from their own biological data Discusses, in detail, raw data files, data processing strategies, and the web based sources relevant for data processing


Handbook of Biomedical Nonlinear Optical Microscopy

Handbook of Biomedical Nonlinear Optical Microscopy
Author: Barry R. Masters
Publisher: Oxford University Press
Total Pages: 895
Release: 2008-05-19
Genre: Science
ISBN: 0198036825

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The Handbook of Biomedical Nonlinear Optical Microscopy provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy for cell biologists, life scientists, biomedical engineers, and clinicians. The chapters are separated into basic and advanced sections, and provide both textual and graphical illustrations of all key concepts. The more basic sections are aimed at life scientists without advanced training in physics and mathematics, and tutorials are provided for the more challenging sections. The first part of the Handbook introduces the historical context of nonlinear microscopy. The second part presents the nonlinear optical theory of two- and multiphoton excited fluorescence (TPE, MPE) spectroscopy, second and third harmonic generation (SHG, THG) spectroscopy, and coherent anti-Stokes Raman spectroscopy (CARS). The third part introduces modern microscopic and spectroscopic instrumentation and techniques that are based on nonlinear optics. The fourth part provides key applications of nonlinear microscopy to the biomedical area: neurobiology, immunology, tumor biology, developmental biology, dermatology, and cellular metabolism. There are also chapters on nonlinear molecular probes, cellular damage, and nanoprocessing.


Cell Biological Applications of Confocal Microscopy

Cell Biological Applications of Confocal Microscopy
Author:
Publisher: Academic Press
Total Pages: 380
Release: 1993-11-17
Genre: Science
ISBN: 0080859364

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Cell Biological Applications of Confocal Microscopy instructs researchers on methods of preparing biological material for confocal microscopy. Individual chapters describe the technical problems of imaging structures in thick specimens and useful techniques such as multi-color dye visualization. The book provides specific examples of applications of confocal microscopy to cell biological problems. This volume is an essential guide for students and scientists incell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. Describes proper specimen preparation for obtaining high-quality data and images Covers pitfalls in imaging different specimens and in employing different dyes Contains detailed protocols accessible to new users Chapters are self-contained units, but describe strategies that can be combined to develop new protocols Techniques are illustrated with full-color plates


Cell Biological Applications of Confocal Microscopy

Cell Biological Applications of Confocal Microscopy
Author:
Publisher: Academic Press
Total Pages: 521
Release: 2003-01-03
Genre: Science
ISBN: 0080925359

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This volume of the acclaimed Methods in Cell Biology series provides specific examples of applications of confocal microscopy to cell biological problems. It is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. An integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM). Includes detailed protocols accessible to new users Details how to set up and run a "Confocal Microscope Core Facility" Contains over 170 figures