Glycolytic Metabolism Plays A Functional Role In Regulating Human Pluripotent Stem Cell State PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Glycolytic Metabolism Plays A Functional Role In Regulating Human Pluripotent Stem Cell State PDF full book. Access full book title Glycolytic Metabolism Plays A Functional Role In Regulating Human Pluripotent Stem Cell State.
Author | : Wen Gu |
Publisher | : |
Total Pages | : 132 |
Release | : 2017 |
Genre | : |
ISBN | : |
Download Glycolytic Metabolism Plays a Functional Role in Regulating Human Pluripotent Stem Cell State Book in PDF, ePub and Kindle
The rate of glycolytic metabolism changes during differentiation of human embryonic stem cells (hESCs) and reprogramming of somatic cells to pluripotent stem cells. However, the functional contribution of glycolytic metabolism to pluripotency is unclear. Here we show that the degree of pluripotency is associated with glycolytic rate, whereby naive hESCs exhibit increased glycolytic flux, MYC transcriptional activity, and nuclear localization of N-MYC relative to primed hESCs. This is consistent with the inner cell mass of human blastocysts which exhibit increased MYC transcriptional activity relative primed hESCs and elevated nuclear N-MYC levels. Reduction of glycolysis decreases self-renewal of naive hESCs and feeder-free cultured primed hESCs, but not primed hESCs grown in feeder-supported conditions. Reduction of glycolysis in feeder-free primed hESCs also enhances neural specification. These findings reveal associations between glycolytic metabolism and the state of pluripotency, differences in the metabolism of feeder- versus feeder-free cultured hESCs, and identify methods for regulating self-renewal and initial cell fate specification of hESCs.
Author | : Carlos Simón |
Publisher | : Cambridge University Press |
Total Pages | : 199 |
Release | : 2013-07-04 |
Genre | : Medical |
ISBN | : 1107034477 |
Download Stem Cells in Reproductive Medicine Book in PDF, ePub and Kindle
Stem cell science has the potential to impact human reproductive medicine significantly - cutting edge technologies allow the production and regeneration of viable gametes from human stem cells offering potential to preciously infertile patients. Written by leading experts in the field Stem Cells in Reproductive Medicine brings together chapters on the genetics and epigenetics of both the male and female gametes as well as advice on the production and regeneration of gene cells in men and women, trophoblasts and endometrium from human embryonic and adult stem cells. Although focussing mainly on the practical elements of the use of stem cells in reproductive medicine, the book also contains a section on new developments in stem cell research. The book is essential reading for reproductive medicine clinicians, gynecologists and embryologists who want to keep abreast of practical developments in this rapidly developing field.
Author | : Carlos M. Palmeira |
Publisher | : Humana |
Total Pages | : 318 |
Release | : 2021-06-08 |
Genre | : Science |
ISBN | : 9781071614327 |
Download Mitochondrial Regulation Book in PDF, ePub and Kindle
This fully updated edition explores the different pathways that converge into the regulation of mitochondrial function. The book integrates mitochondria with other cellular components, discussing the dynamic properties of mitochondria with an emphasis on how these processes respond to signaling events and how they affect cellular metabolism. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Mitochondrial Regulation: Methods and Protocols, Second Edition is an ideal guide for advanced undergraduates, graduates, postgraduates, and beginning researchers in the areas of molecular and cellular biology, biochemistry, and bioenergetics.
Author | : Amy A. Ferreccio |
Publisher | : |
Total Pages | : 47 |
Release | : 2017 |
Genre | : |
ISBN | : |
Download Regulation of Stemness in Humans Book in PDF, ePub and Kindle
Author | : |
Publisher | : Academic Press |
Total Pages | : 523 |
Release | : 2011-11-23 |
Genre | : Science |
ISBN | : 0123860164 |
Download Stem Cell Regulators Book in PDF, ePub and Kindle
First published in 1943, Vitamins and Hormones is the longest-running serial published by Academic Press. The Editorial Board now reflects expertise in the field of hormone action, vitamin action, X-ray crystal structure, physiology and enzyme mechanisms. Under the capable and qualified editorial leadership of Dr. Gerald Litwack, Vitamins and Hormones continues to publish cutting-edge reviews of interest to endocrinologists, biochemists, nutritionists, pharmacologists, cell biologists and molecular biologists. Others interested in the structure and function of biologically active molecules like hormones and vitamins will, as always, turn to this series for comprehensive reviews by leading contributors to this and related disciplines. This volume focuses on stem cell regulators. Longest running series published by Academic Press Contributions by leading international authorities
Author | : Barry D. Bavister |
Publisher | : Springer Science & Business Media |
Total Pages | : 359 |
Release | : 2012-12-06 |
Genre | : Science |
ISBN | : 1461393175 |
Download Preimplantation Embryo Development Book in PDF, ePub and Kindle
This volume contains the Proceedings of the Serono Symposium on Pre implantation Embryo Development, held in Newton, Massachusetts, in 1991. The idea for the symposium grew out of the 1989 Serono Symposium on Fertilization in Mammals* at which preimplantation development was the predominant suggestion for a follow-up topic. This was indeed a timely subject in view of the recent resurgence of interest in this funda mental phase of embryogenesis and its relevance to basic research and applied fertility studies in humans, food-producing animals, and endangered species. The symposium brought together speakers from a broad range of disciplines in order to focus on key regulatory mechanisms in embryo development, using a wide variety of animal models, and on representative topics in human preimplantation embryogenesis. The culmination of preimplantation development is a blastocyst con taining the first differentiated embryonic tissues and capable of initiating and sustaining pregnancy. The central objective of the symposium was to throw light on the regulation of cellular and molecular events underlying blastocyst formation. It was particularly appropriate that the date of the symposium marked the 20th anniversary of the publication of the classic volume Biology of the Blastocyst, the proceedings of an international workshop held in 1970. This book, which summarized most of the information then available on this topic in mammals, was edited by the pioneer in blastocyst research, Dr. Richard B1andau, who was the guest speaker at the symposium.
Author | : Anthony Scimè |
Publisher | : Frontiers Media SA |
Total Pages | : 161 |
Release | : 2022-01-21 |
Genre | : Science |
ISBN | : 2889741346 |
Download The Role of Metabolism in Stem Cell Fates Book in PDF, ePub and Kindle
Author | : Kursad Turksen |
Publisher | : Springer Science & Business Media |
Total Pages | : 504 |
Release | : 2008-02-04 |
Genre | : Science |
ISBN | : 1597450375 |
Download Embryonic Stem Cell Protocols Book in PDF, ePub and Kindle
Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume one, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. The second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.
Author | : Matthew H. Kaufman |
Publisher | : CUP Archive |
Total Pages | : 296 |
Release | : 1983-10-06 |
Genre | : Nature |
ISBN | : 9780521254496 |
Download Early Mammalian Development Book in PDF, ePub and Kindle
Author | : Jon Odorico |
Publisher | : Garland Science |
Total Pages | : 391 |
Release | : 2004-02-01 |
Genre | : Science |
ISBN | : 0203487346 |
Download Human Embryonic Stem Cells Book in PDF, ePub and Kindle
Since the first successful isolation and cultivation of human embryonic stem cells at the University of Wisconsin, Madison in 1998, there has been high levels of both interest and controversy in this area of research. This book provides a concise overview of an exciting field, covering the characteristics of both human embryonic stem cells and pluripotent stem cells from other human cell lineages. The following chapters describe state-of-the-art differentiation and characterization of specific ectoderm, mesoderm and endoderm-derived lineages from human embryonic stem cells, emphasizing how these can be used to study human developmental mechanisms. A further chapter discusses genetic manipulation of human ES cells. The concluding section covers therapeutic applications of human ES cells, as well as addressing the ethical and legal issues that this research have raised.