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| Author | : Christopher W.J. Smith |
| Publisher | : Oxford University Press, UK |
| Total Pages | : 370 |
| Release | : 1998-07-09 |
| Genre | : |
| ISBN | : 0191591629 |
Download RNA-Protein Interactions : A Practical Approach Book in PDF, ePub and Kindle
RNA-protein interactions play a fundamental role in gene expression and protein synthesis. Recent research into the role of RNA in cells has elucidated many more vital interactions with proteins. This book provides an up-to-date and comprehensive guide to a wide range of laboratory procedures to investigate the interactions between RNA and proteins. - ;RNA-protein interactions play a vital role in gene transcription and protein expression. Interactions such as the synthesis of mRNA by RNA polymerases, to the essential modification of RNA by the proteins of the spliceosome complex, and the highly catalytic action of the ribosome in protein synthesis, are established as being fundamental to the function of RNA. Recent research into, for example, the role of RNA as a catalyst, has elucidated many more interactions with proteins that are vital to cell function. RNA - Protein Interactions: A Practical Approach provides a clear and comprehensive guide to the experimental procedures used in studying RNA - protein interactions. The approaches covered range from those initially used to detect a novel RNA-protein interaction, various biochemical and genetic approaches to purifying and cloning RNA binding proteins, through to methods for an in depth analysis of the structural basis of the interaction. The volume includes a number of procedures that have not previously been covered in this type of manual. These include the production of site-specifically modified RNAs by enzymatic and chemical methods and in vivo screening for novel RNA - protein interactions in yeast and E. coli . This is the first volume to gather in one place this wide array of approaches for studying RNA - protein interactions. As is customary for the Practical Approach series, the writing is characterized by a clear explanatory style with many detailed protocols. This informative book will be a valuable aid to laboratory workers in biochemistry and molecular biology - graduate students, postdoctoral and senior scientists - whose research encompasses this field. -
| Author | : André Gerber |
| Publisher | : Sudwestdeutscher Verlag Fur Hochschulschriften AG |
| Total Pages | : 64 |
| Release | : 2011-12 |
| Genre | : |
| ISBN | : 9783838113388 |
Download Global Analysis of Protein-RNA Interactions Book in PDF, ePub and Kindle
It is becoming increasingly evident that post-transcriptional regulation has profound impact on when, where, and to what level genes are expressed. Hundreds of RNA-binding proteins and non-coding RNAs mediate post-transcriptional gene regulation - but how they are coordinated and what their RNA targets are remains largely elusive. The development of genome-wide analysis tools enables us now to systematically study the extent and logic of post-transcriptional gene regulation on global scale. In this book, the author Andre Gerber explains some of these new techniques, which led to the discovery of novel principles of gene expression control such as RNA regulons. He also provides an overview of current knowledge about the implications of RNA-binding proteins and non-coding RNAs in human disease, and the chances and pitfalls towards the development of novel therapeutics. This book is written for everyone with basic knowledge in molecular biology and who wishes to get an introduction into this fascinating field of RNA research."
| Author | : |
| Publisher | : |
| Total Pages | : |
| Release | : 2010 |
| Genre | : |
| ISBN | : |
Download Global Analysis of Protein-RNA Interactions Book in PDF, ePub and Kindle
| Author | : Mathias Munschauer |
| Publisher | : Springer |
| Total Pages | : 140 |
| Release | : 2015-03-14 |
| Genre | : Technology & Engineering |
| ISBN | : 3319162535 |
Download High-Resolution Profiling of Protein-RNA Interactions Book in PDF, ePub and Kindle
The work reported in this book represents an excellent example of how creative experimentation and technology development, complemented by computational data analysis, can yield important insights that further our understanding of biological entities from a systems perspective. The book describes how the study of a single RNA-binding protein and its interaction sites led to the development of the novel ‘protein occupancy profiling’ technology that for the first time captured the mRNA sequence space contacted by the ensemble of expressed RNA binders. Application of protein occupancy profiling to eukaryotic cells revealed that extensive sequence stretches in 3’ UTRs can be contacted by RBPs and that evolutionary conservation as well as negative selection act on protein-RNA contact sites, suggesting functional importance. Comparative analysis of the RBP-bound sequence space has the potential to unravel putative cis-acting RNA elements without a priori knowledge of the bound regulators. Here, Dr. Munschauer provides a comprehensive introduction to the field of post-transcriptional gene regulation, examines state-of-the-art technologies, and combines the conclusions from several journal articles into a coherent and logical story from the frontiers of systems-biology inspired life science. This thesis, submitted to the Department of Biology, Chemistry and Pharmacy at Freie Universität Berlin, was selected as outstanding work by the Berlin Institute for Medical Systems Biology at the Max-Delbrueck Center for Molecular Medicine, Germany.
| Author | : Susan R. Haynes |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 485 |
| Release | : 2008-02-03 |
| Genre | : Science |
| ISBN | : 1592596762 |
Download RNA'Protein Interaction Protocols Book in PDF, ePub and Kindle
The molecular characterization of RNA and its interactions with proteins is an important and exciting area of current research. Organisms utilize a variety of RNA–protein interactions to regulate the expression of their genes. This is particularly true for eukaryotes, since newly synthesized messenger RNA must be extensively modified and transported to the cytoplasm before it can be used for protein synthesis. The realization that posttranscriptional processes are critical components of gene regulation has sparked an explosion of interest in both stable ribonucleoprotein (RNP) complexes and transient RNA–protein interactions. RNA is conformationally flexible and can adopt complex structures that provide diverse surfaces for interactions with proteins. The fact that short RNA molecules (aptamers; see Chapter 16) can be selected to bind many different types of molecules is evidence of the structural variability of RNA. RNA molecules are rarely entirely single- or double-stranded, but usually contain multiple short duplexes interrupted by single-stranded loops and bulges; in some RNAs, such as tRNAs, the short duplexes stack on each other. Further variability is generated by the presence of non-Watson-Crick base pairs, modified nucleotides, and more complex structures, such as pseudoknots and triple-strand interactions.
| Author | : Roberto Giambruno |
| Publisher | : Frontiers Media SA |
| Total Pages | : 123 |
| Release | : 2022-02-11 |
| Genre | : Science |
| ISBN | : 2889743691 |
Download The Role of Protein Post-Translational Modifications in Protein-RNA Interactions and RNP Assemblies Book in PDF, ePub and Kindle
Dr. Nicolas Lux Fawzi is a member of the Scientific Advisory Board of Dewpoint Therapeutics LLC. All other Topic Editors declare no competing interests with regards to the Research Topic.
| Author | : Ren-Jang Lin |
| Publisher | : Springer Nature |
| Total Pages | : 349 |
| Release | : 2023-05-11 |
| Genre | : Science |
| ISBN | : 1071631918 |
Download RNA-Protein Complexes and Interactions Book in PDF, ePub and Kindle
This second edition updates, complements, and expands upon the first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. Chapters explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA-Protein Complexes and Interactions: Methods and Protocols, Second Edition aims to be comprehensive guide for researchers in the field.
| Author | : Kiyoshi Nagai |
| Publisher | : Oxford University Press, USA |
| Total Pages | : 302 |
| Release | : 1994 |
| Genre | : Medical |
| ISBN | : |
Download RNA-protein Interactions Book in PDF, ePub and Kindle
The study of RNA-protein interactions is crucial to understanding the mechanisms and control of gene expression and protein synthesis. The realization that RNAs are often far more biologically active than was previously appreciated has stimulated a great deal of new research in this field. Uniquely, in this book, the world's leading researchers have collaborated to produce a comprehensive and current review of RNA-protein interactions for all scientists working in this area. Timely, comprehensive, and authoritative, this new Frontiers title will be invaluable for all researchers in molecular biology, biochemistry and structural biology.
| Author | : |
| Publisher | : |
| Total Pages | : 303 |
| Release | : 1966 |
| Genre | : |
| ISBN | : |
Download I gruppi dirigenti in Europa occidentale tra dimensione nazionali e dimensioni sopranazionali Book in PDF, ePub and Kindle
| Author | : Alexandros Armaos |
| Publisher | : |
| Total Pages | : 110 |
| Release | : 2020 |
| Genre | : |
| ISBN | : |
Download Computational Characterization of Protein-RNA Interactions and Implications for Phase Separation Book in PDF, ePub and Kindle
Despite what was previously considered, the role of RNA is not only to carry the geneticinformation from DNA to proteins. Indeed, RNA has proven to be implicated in morecomplex cellular processes. Recent evidence suggests that transcripts have a regulatoryrole on gene expression and contribute to the spatial and temporal organization of theintracellular environment. They do so by interacting with RNA-binding proteins (RBPs)to form complex ribonucleoprotein (RNP) networks, however the key determinants thatgovern the formation of these complexes are still not well understood. In this work, I willdescribe algorithms that I developed to estimate the ability of RNAs to interact withproteins. Additionally, I will illustrate applications of computational methods to proposean alternative model for the function of Xist lncRNA and its protein network.Finally, I will show how computational predictions can be integrated with highthroughput approaches to elucidate the relationship between the structure of the RNA andits ability to interact with proteins. I conclude by discussing open questions and futureopportunities for computational analysis of cell's regulatory network.Overall, the underlying goal of my work is to provide biologists with new insights intothe functional association between RNAs and proteins as well as with sophisticated toolsthat will facilitate their investigation on the formation of RNP complexes.
