Genetic Library Construction And Screening PDF Download
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Author | : R.C. Bird |
Publisher | : Springer Science & Business Media |
Total Pages | : 244 |
Release | : 2012-12-06 |
Genre | : Science |
ISBN | : 3642564089 |
Download Genetic Library Construction and Screening Book in PDF, ePub and Kindle
Designed as an introductory text the authors cover all core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies. The handbook contains detailed step-by-step protocols and extensive hands-on advice.
Author | : Wei Xiao |
Publisher | : Humana |
Total Pages | : 0 |
Release | : 2016-09-17 |
Genre | : Science |
ISBN | : 9781493942770 |
Download Yeast Protocols Book in PDF, ePub and Kindle
Yeast Protocols, Third Edition presents up-to-date advances in research using yeasts as models. Chapters cover topics such as basic protocols in yeast culture and genomic manipulation, protocols that study certain organelles such as mitochondria and peroxisomes and their functions in autophagy and assays commonly used in yeast-based studies that can be adapted to other organisms. As the first sequenced living organism, budding yeast S. cerevisiae and other model yeasts have helped greatly in life science research. The easy switch between the haploid and diploid state makes yeast a paradigm of genetic manipulation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Yeast Protocols, Third Edition seeks to serve both professionals and novices with newly-developed protocols to study this essential model organism.
Author | : A. Curtis |
Publisher | : |
Total Pages | : |
Release | : 1983 |
Genre | : |
ISBN | : |
Download Construction and Screening of a Chicken Genomic DNA Library for Myosin Heavy Chain Gene Sequences Book in PDF, ePub and Kindle
Author | : Ian G. Cowell |
Publisher | : Springer Science & Business Media |
Total Pages | : 324 |
Release | : 2008-02-02 |
Genre | : Science |
ISBN | : 1592595553 |
Download cDNA Library Protocols Book in PDF, ePub and Kindle
The first libraries of complementary DNA (cDNA) clones were con structed in the mid-to-late 1970s using RNA-dependent DNA polymerase (reverse transcriptase) to convert poly A* mRNA into double-stranded cDNA suitable for insertion into prokaryotic vectors. Since then cDNA technology has become a fundamental tool for the molecular biologist and at the same time some very significant advances have occurred in the methods for con structing and screening cDNA libraries. It is not the aim of cDNA Library Protocols to give a comprehensive review of all cDNA library-based methodologies; instead we present a series of up-to-date protocols that together should give a good grounding of proce dures associated with the construction and use of cDNA libraries. In deciding what to include, we endeavored to combine up-to-date versions of some of the most widely used protocols with some very usefiil newer techniques. cDNA Library Protocols should therefore be especially useful to the investigator who is new to the use of cDNA libraries, but should also be of value to the more experienced worker. Chapters 1—5 concentrate on cDNA library construction and manipula tion, Chapters 6 and 7 describe means of cloning difficult-to-obtain ends of cDNAs, Chapters 8-18 give various approaches to the screening of cDNA libraries, and the remaining chapters present methods of analysis of cDNA clones including details of how to analyze cDNA sequence data and how to make use of the wealth of cDNA data emerging from the human genome project.
Author | : Sukanta Mondal |
Publisher | : Academic Press |
Total Pages | : 340 |
Release | : 2020-11-25 |
Genre | : Science |
ISBN | : 0128206128 |
Download Advances in Animal Genomics Book in PDF, ePub and Kindle
Advances in Animal Genomics provides an outstanding collection of integrated strategies involving traditional and modern - omics (structural, functional, comparative and epigenomics) approaches and genomics-assisted breeding methods which animal biotechnologists can utilize to dissect and decode the molecular and gene regulatory networks involved in the complex quantitative yield and stress tolerance traits in livestock. Written by international experts on animal genomics, this book explores the recent advances in high-throughput, next-generation whole genome and transcriptome sequencing, array-based genotyping, and modern bioinformatics approaches which have enabled to produce huge genomic and transcriptomic resources globally on a genome-wide scale. This book is an important resource for researchers, students, educators and professionals in agriculture, veterinary and biotechnology sciences that enables them to solve problems regarding sustainable development with the help of current innovative biotechnologies. Integrates basic and advanced concepts of animal biotechnology and presents future developments Describes current high-throughput next-generation whole genome and transcriptome sequencing, array-based genotyping, and modern bioinformatics approaches for sustainable livestock production Illustrates integrated strategies to dissect and decode the molecular and gene regulatory networks involved in complex quantitative yield and stress tolerance traits in livestock Ensures readers will gain a strong grasp of biotechnology for sustainable livestock production with its well-illustrated discussion
Author | : B.N. Pandey |
Publisher | : APH Publishing |
Total Pages | : 426 |
Release | : 2008 |
Genre | : Biotechnology |
ISBN | : 9788131303061 |
Download Biotechnology Book in PDF, ePub and Kindle
Author | : L. L. De Verno |
Publisher | : Chalk River, Ont. : Forestry Canada, Petawawa National Forestry Institute ; Ottawa : Ministry of Supply and Services Canada |
Total Pages | : 36 |
Release | : 1989 |
Genre | : Conifers |
ISBN | : |
Download Constructing Conifer Genomic Libraries Book in PDF, ePub and Kindle
Procedure for constructing genomic DNA bacteriophage libraries from conifer tissue. Although black spruce B.S.P. tissue was used, the protocols can be applied to any plant species. The manual includes techniques for high molecular weight genomic DNA isolation from mature needles, seedlings, and cell suspension cultures; purification of size-fractionated genomic DNA fragments; vector DNA isolation; preparation of vector arms; production of recombinant clones; in vitro packaging; amplification; evaluation of library quality; methods for purification of sized fragments; media and buffer formulas; and the number of recombinants required to represent genomes of various species.
Author | : Shaying Zhao |
Publisher | : |
Total Pages | : 0 |
Release | : 2004 |
Genre | : Bacterial artificial chromosomes |
ISBN | : |
Download Bacterial Artificial Chromosomes Book in PDF, ePub and Kindle
Author | : Bruce Alberts |
Publisher | : |
Total Pages | : 0 |
Release | : 2002 |
Genre | : Cytology |
ISBN | : 9780815332183 |
Download Molecular Biology of The Cell Book in PDF, ePub and Kindle
Author | : Alister G. Craig |
Publisher | : Elsevier |
Total Pages | : 296 |
Release | : 1999-02 |
Genre | : Science |
ISBN | : 9780121948603 |
Download Automation: Genomic and Functional Analyses Book in PDF, ePub and Kindle
Evaluation and functional analysis, to provide insight into this "new age" of research based on genomic and chemical screening. Key Features * Describes automated procedures used in microbiology and molecular biology * Includes developments in robotics and vision systems * Features automation in library picking, presentation and analysis * Discusses paralogous duplications in microbial genomes * Covers deciphering genomes through automated large-scale sequencing * Describes and stresses the need for functional analyses * Internationally acclaimed contributors, including Professor Leroy Hood.