Genetic Approaches To Investigation Of The Rpreduced Pigment Gene In Carrotdaucus Carota L PDF Download

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Gene Expression and Genetic Analysis of Carotenoid Pigment Accumulation in Carrot (Daucus Carota L.)

Gene Expression and Genetic Analysis of Carotenoid Pigment Accumulation in Carrot (Daucus Carota L.)
Author:
Publisher:
Total Pages: 510
Release: 2012
Genre:
ISBN:

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Carrot (Daucus carota) is an important vegetable crop, providing over 30% of the pro-vitamin A carotenoids in the human diet in the United States. As a major source of these nutriceutical compounds, research efforts have been directed to the regulation of this complex metabolic pathway. This project focused on evaluating gene expression, sequence changes and genetic mapping as it relates to the accumulation of the carotenoid pigments alpha-carotene, beta-carotene, xanthophylls, and lutein. Real-time quantitative PCR (RT-qPCR) was used to determine the level of expression of genes in the carotenoid biosynthesis pathway in different carrot culitvars. Two genes, phytoene synthase 1 (PSY1) and phytoene synthase 2 (PSY2) were found to be expressed at significantly higher levels in orange and dark orange carrot cultivars, whereas the pale orange rp "reduced pigment" mutant exhibited reduced phytoene desaturase (PDS) expression. DNA sequence analysis of putative promoter and coding regions of both PSY1 and PSY2 has identified sequence polymorphisms and large deletions were identified in the putative promoter of PSY2 in the dark orange cultivar B2327. Simple sequence repeat markers (SSRs) were used to create a genetic map from an F2 population segregating for the rp mutation. HPLC (high performance liquid chromatography) analyses of the carotenoids of carrot leaf tissue demonstrated xanthophylls and beta-carotene as the carotenoids present in all carrots, but significantly higher levels of alpha-carotene was identified in carrot germplasm with orange and dark orange storage roots. Chlorophyll fluorescence analysis identified genotype specific variation in photosystem II efficiency, significantly impacted by the time of harvest. To identify new candidate genes involved in carotenoid accumulation in carrot storage root, microarray analysis of sibling white and orange inbred lines from a cross of orange X white carrots was completed. Expression of three genes involved in photosynthesis was significantly higher in the orange rooted carrot germplasm. To examine the role that chromoplast biosynthesis may have in the accumulation of carotenoid pigments, the ratio of nuclear to plastid genome was analyzed in carrot germplasm with differing levels of pigmentation.


The Science of Flavonoids

The Science of Flavonoids
Author: Erich Grotewold
Publisher: Springer Science & Business Media
Total Pages: 300
Release: 2008
Genre: Science
ISBN: 9780387745503

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This is the only book of its kind to provide an overview of the science of flavonoids in plants.


Anthocyanins

Anthocyanins
Author: Kevin Gould
Publisher: Springer Science & Business Media
Total Pages: 345
Release: 2008-12-19
Genre: Science
ISBN: 0387773355

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In recent years there has been an unprecedented expansion of knowledge about anthocyanins pigments. Indeed, the molecular genetic control of anthocyanins biosynthesis is now one of the best understood of all secondary metabolic pathways. There have also been substantial improvements in analytical technology that have led to the discovery of novel anthocyanin compounds. Armed with this knowledge and the tools for genetic engineering, plant breeders are now introducing vibrant new colors into horticultural crops. The food industry has also benefited from the resurgence of interest in anthocyanins. A greater understanding of the chemistry of these pigments has led to improved methods for stabilizing the color of anthocyanins extracts, so that they are more useful as food colorings. Methods for the bulk production of anthocyanins from cell cultures have been optimized for this purpose. Possible benefits to human health from the ingestion of anthocyanin-rich foods have also been a major feature of the recent scientific literature. Anthocyanins are remarkably potent antioxidants, and their ingestion has been postulated to stave off the effects of oxidative stress. These pigments, especially in conjunction with other flavonoids, have been associated with reductions in the incidence and severity of many other non-infectious diseases, including diabetes, cardiovascular disease and certain cancers. An industry is developing around anthocyanins as nutritional supplements. Finally, there has been significant progress in our understanding of the benefits of anthocyanins to plants themselves. Originally considered an extravagance without a purpose, anthocyanins are now implicated in multifarious vital functions. These include the attraction of pollinators and frugivores, aposematic defense from herbivores, and protection from environmental stressors such as strong light, UVB, drought, and free radical attacks. Anthocyanins are evidently highly versatile, and enormously useful to plants. This book covers all aspects of the biosynthesis and function of anthocyanins (and related compounds such as proanthocyanidins) in plants, and their applications in agriculture, food products, and human health. Featured areas include their relevance to: * Plant stress * Flower and fruit color * Human health * Wine quality and health attributes * Food colorants and ingredients * Cell culture production systems * The pastoral sector


Vitamins and Minerals Biofortification of Edible Plants

Vitamins and Minerals Biofortification of Edible Plants
Author: Noureddine Benkeblia
Publisher: John Wiley & Sons
Total Pages: 255
Release: 2020-05-26
Genre: Technology & Engineering
ISBN: 1119511119

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A Detailed Reference on How Modern Biotechnology is using the Biofortification of Crops to Improve the Vitamin and Mineral Content of Edible Plants In this reference, Vitamins and Minerals Bio-Fortification of Edible Plants, authors cover new territory on phytonutrients, focusing on the enhancement and modification of edible crops. This book presents techniques and research findings from modern biotechnology to educate readers on the newest tools and research in the field. Readers will learn how groundbreaking scientific advances have contributed to the nutritional content of edible plants and crops for animals and humans. Inside, readers will find comprehensive information on new concepts of biofortification, including but not limited to: ● Modern biotechnology and its uses for improving the vitamin and mineral content of edible plants ● Potential minerals and vitamins that can be targeted and implemented in agriculture ● Ways of enhancing the nutritional contents of edible plants to address nutritional deficiencies and improve livestock ● Methods of identifying plants that can be used to heal or prevent disease and illness While many books cover the phytonutrients of crops, this reference book reports on methodologies, techniques, and environmental changes used to enhance and improve agricultural products. It is one of the first to provide information on using modern biotechnologies to modify crops with the goal of creating health benefits.


Genome Editing in Plants

Genome Editing in Plants
Author: Om Prakash Gupta
Publisher: CRC Press
Total Pages: 382
Release: 2021-07-25
Genre: Science
ISBN: 1000410196

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Genome Editing in Plants: Principles and Applications addresses the information of genome editing starting from principles and historical aspects to the latest advancements in the field. As genome-editing technology has emerged as promising and cutting edge, researchers around the world have started producing original research outputs, which have significantly improved our current understanding and potential of this technology. The initial chapters of this book describe different genome-editing tools as well as their principles and applications. Other chapters are dedicated to the present status and future applications of genome-editing techniques in various crop improvement programmes. Some of the advanced applications of CRISPR/Cas tools, such as base editing and RNA detection, along with regulatory aspects of genome-edited crops are described in detail. This book serves as a valuable resource to researchers in the field of crop improvement; graduate and postgraduate students engaged in plant molecular biology and biotechnology; academicians; and policy makers. Key Features: Addresses topics associated with historical development and principles of genome-editing technology Addresses basic mechanisms operating under each genome-editing technology Addresses its application in plants to design crops as per the current and future demands Addresses the regulatory mechanisms of genome-edited crops


Carotenoids in Nature

Carotenoids in Nature
Author: Claudia Stange
Publisher: Springer
Total Pages: 454
Release: 2016-08-02
Genre: Science
ISBN: 3319391267

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This comprehensive, edited book explores carotenoids and their important functional roles in yeast, bacteria and plants and a profound exposition on the structures of carotenoid molecules, focusing in the first of three parts on the biosynthesis of carotenoids. The regulation of carotenoid biosynthesis in photosynthesis as well as in plant, fruits, storage roots and algae is central to the second part, and discoveries about the function of carotenoids in human health feature in the third and final part. Many helpful illustrations, explanations, overviews and examples help to bring readers up to date on relevant themes including carotenogenic genes, carotenoids in fruits and metabolic engineering. The book explores where carotenoids are synthesized in nature, including in carrots and algae. Contributing expert authors examine enzyme functions and plant models, and analyze the structure of carotenoid molecules. The function of carotenoids in photosynthesis and in photosynthetic organs as well as during fruit ripening are then explored. A whole chapter is dedicated to the latest research on apocarotenoids and further chapters cover interesting and novel themes on plastid development and the epigenetic regulation that affects carotenoid synthesis in plants. The metabolic engineering of carotenoids that has been done in fruits, plants, and seeds is another area that readers can explore, along with evidences on the function of carotenoids in human nutrition, as antioxidants, as in the control of lipid metabolism and in the absorption of carotenoids. This is a highly informative and wide-ranging work which will update researchers in the field, as well as supporting students of plant physiology and biotechnology, as supplementary reading.


Handbook of Vegetable Preservation and Processing

Handbook of Vegetable Preservation and Processing
Author: Y. H. Hui
Publisher: CRC Press
Total Pages: 766
Release: 2003-09-12
Genre: Technology & Engineering
ISBN: 9780203912911

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Representing the vanguard in the field with research from more than 35 international experts spanning governmental, industrial, and academic sectors, the Handbook of Vegetable Preservation and Processing compiles the latest science and technology in the processing and preservation of vegetables and vegetable products. This reference serves as the only guide to compile key tools used in the United States to safeguard and protect the quality of fresh and processed vegetables. A vast and contemporary source, it considers recent issues in vegetable processing safety such as modified atmosphere packaging, macroanalytical methods, and new technologies in microbial inactivation.


Pigments in Vegetables

Pigments in Vegetables
Author: Jeana Gross
Publisher: Springer Science & Business Media
Total Pages: 361
Release: 2012-12-06
Genre: Science
ISBN: 1461520339

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Advances in Plant Breeding Strategies: Vegetable Crops

Advances in Plant Breeding Strategies: Vegetable Crops
Author: Jameel M. Al-Khayri
Publisher: Springer Nature
Total Pages: 559
Release: 2021-08-24
Genre: Technology & Engineering
ISBN: 3030669653

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This book examines the development of innovative modern methodologies towards augmenting conventional plant breeding, in individual crops, for the production of new crop varieties under the increasingly limiting environmental and cultivation factors to achieve sustainable agricultural production, enhanced food security, in addition to providing raw materials for innovative industrial products and pharmaceuticals. This Volume 8, subtitled Vegetable Crops: Bulbs, Roots and Tubers, consists of 12 chapters focusing on advances in breeding strategies using both traditional and modern approaches for the improvement of individual vegetable crops. Chapters are arranged in 3 parts according to the edible vegetable parts. Part I: Bulbs - Garlic (Allium sativum L.), Leek (Allium ampeloprasum L.) and Shallot (Allium cepa L. Aggregatum group); Part II: Roots - Beetroot (Beta vulgaris ssp. vulgaris var. conditiva Alefeld), Carrot (Daucus carota L.), Parsnip (Pastinaca sativa L.), Radish (Raphanus sativus L.), Sugar beet (Beta vulgaris ssp. vulgaris L.) and Turnip (Brassica rapa var. rapa L.), Part III: Tubers - Potato (Solanum tuberosum L.) and Sweet potato (Ipomea batatas L.). The chapters were contributed by 38 internationally reputable scientists from 13 countries. Each chapter comprehensively reviews the modern literature on the subject and reflects the authors own experience.