Genetic And Phenotypic Analysis Of Clk 1 Growth Suppressors In Caenorhabditis Elegans PDF Download
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Author | : Thi Phuong Anh Nguyen |
Publisher | : |
Total Pages | : 204 |
Release | : 2005 |
Genre | : Caenorhabditis elegans |
ISBN | : |
Download Genetic and Phenotypic Analysis of Clk-1 Growth Suppressors in Caenorhabditis Elegans Book in PDF, ePub and Kindle
"Ubiquinone (UQ) is a lipid found in all cellular membranes. It is involved in multiple cellular processes, either directly or through its effect on the redox status of the cell. clk-1 encodes a highly conserved hydroxylase required for UQ biosynthesis. In C. elegans, mutations in clk-1 result in the accumulation of an UQ precursor, DMQ, and a pleiotropic phenotype in the mutants characterized by the slowing down of development, behaviors and aging. Additionally, in the absence of dietary UQ, clk-1 mutants also show a transient growth arrest and are sterile. Mutants that can suppress both sets of phenotypes in the point mutant clk-1(e2519) have been isolated. Their suppression patterns indicate that various aspects of the clk-1 phenotype can be uncoupled from each other. Furthermore, the analysis of their quinone content suggests that the phenotypes on UQ-producing bacteria are caused by the inability of dietary UQ to completely substitute for endogenous UQ. These suppressors carry mutations in tRNA genes, and thus to our knowledge, they are the first tRNA missense suppressors found in any metazoan." --
Author | : Jason Burgess |
Publisher | : |
Total Pages | : 190 |
Release | : 2002 |
Genre | : Caenorhabditis elegans |
ISBN | : |
Download Phenotypic and Molecular Analysis of the Maternal Effect Associated with Mutations in the Clk-1 Gene of Caenorhabditis Elegans Book in PDF, ePub and Kindle
"Mutations in the Caenorhabditis elegans maternal-effect gene clk-1 result in a highly pleiotropic phenotype, characterized by a general slow down in embryonic and larval development, as well as a slowing down of adult behaviors including defecation, pharyngeal pumping and swimming. First generation homozygous clk-1 mutants descended from a heterozygous mother are fully rescued for these mutant phenotypes. It has been shown that CLK-1 protein is a hydroxylase that acts in the conversion of demethoxyubiquinone (DMQ) to 5-hydroxyubiquinone, in the ubiquinone (Q) biosynthesis pathway. Consequently, clk-1 mutants accumulate the Q9 precursor, DMQ9 (the subscript refers to the length of the isoprenoid side chain). Here, I show that the profound maternal rescue observed in clk-1 maternally rescued animals is due to presence of the CLK-1 protein throughout larval development, in sufficient amounts to catalyze the production of Q9. clk-1 mutants have been shown to have a dietary requirement for Q8 due to their inability to synthesize Q9. I demonstrate that clk-1 maternally rescued animals have sufficient amounts of Q 9 to complete larval development and produce an almost full brood when raised on a Q8 deficient E. coli strain. I also show that prolonged arrest at the first larval stage, which is likely to result in degradation of any maternally contributed mRNA or protein, brings about a Clk mutant phenotype in maternally rescued animals. Finally, I reveal that the Clk mutant phenotype can be rescued at any larval stage by ectopic expression of CLK-1, suggesting that there is no developmental window for the rescue of clk-1 mutants by CLK-1. These results identify perdurance of maternally contributed product throughout development as the mechanism that accounts for the maternal effect observed in clk-1 mutants." --
Author | : Darya Kryzskaya |
Publisher | : |
Total Pages | : |
Release | : 2011 |
Genre | : |
ISBN | : |
Download Genetic Interactions Between Suppressors of the Slow Defecation of Phenotype of Clk-1 Mutants Book in PDF, ePub and Kindle
Many genes that are involved in nutrient and energy utilization and storage are conserved between humans and C. elegans. One of the genes involved in worm mitochondrial respiration is clk-1. This gene encodes an enzyme required for ubiquinone biosynthesis. One of the phenotypes of clk-1 mutants is altered defecation cycle: the slowing down of the defecation cycle and the inability to defecate faster at higher temperatures. these phenotypes are independent of one another. Several lines of evidence suggest that the slowed defecation of clk-1 mutants is due to alterations in lipoprotein/cholesterol metabolism because the mutant phenotype can be suppressed by the reduction of dietary cholesterol, administration of drugs affecting lipoprotein metabolism, and mutating genes involved in lipoprotein metabolism. These mutations can be separated into two classes: those which suppress the clk-1 slow defecation at 20 as well as after the shift to 25°C (class I) and those ...
Author | : Robyn Branicky |
Publisher | : |
Total Pages | : 428 |
Release | : 2006 |
Genre | : Caenorhabditis elegans |
ISBN | : |
Download Suppressor Analysis of the Clk-1 Mutants of Caenorhabditis Elegans Book in PDF, ePub and Kindle
"clk-1 encodes a hydroxylase that is necessary for ubiquinone (UQ) biosynthesis. clk-1 mutants do not synthesize UQ, but instead accumulate the precursor demethoxyubiquinone (DMQ). When fed on bacteria that synthesize UQ the mutants are viable but display slow development, behaviours and aging. However, they arrest development when fed on UQ synthesis-deficient bacteria. I have taken a genetic suppressor approach to investigate the causes of the various phenotypes as well as of the dietary requirements of the clk-1 mutants." --
Author | : Malini Chawla Gupta |
Publisher | : |
Total Pages | : 342 |
Release | : 1996 |
Genre | : |
ISBN | : |
Download Analysis of the Alpha-1(IV) Collagen Gene Emb-9 and Its Suppressors in C. Elegans Book in PDF, ePub and Kindle
Author | : Paula Ruth Martin |
Publisher | : |
Total Pages | : 114 |
Release | : 1991 |
Genre | : |
ISBN | : |
Download Genetic and Phenotypic Analysis of Granchildless Loci in Caenorhabditis Elegans Book in PDF, ePub and Kindle
Author | : Stephanie Felkai |
Publisher | : |
Total Pages | : 68 |
Release | : 1998 |
Genre | : Caenorhabditis elegans |
ISBN | : |
Download Phenotypic Consequences of Altering Expression of the Caenorhabditis Elegans Timing Gene Clk-1 Book in PDF, ePub and Kindle
"clk-1 mutants of the nematode C. elegans have been phenotypically characterized in previous work and found to have decreased rates of development and of periodic adult behaviours, such as defecation, pumping and swimming (Wong et al., 1995). In this study, the defecation periods in older wild type and clk-1 mutant worms were found to be similar despite their differences in young adults. Transgenic strains which express high levels of clk-1 were characterized phenotypically. Over-expression of the clk-1 gene has no observed effect on physiological rates as measured conventionally in young adults but rather has an effect on defecation rates in an age-dependent manner. A proportion of ageing transgenic animals over-expressing clk-1 did not have decreased defecation period, as seen in ageing wild type and clk-1 mutants. This suggests that the action of CLK-1, which functions in controlling physiological rates, is reduced in older wild type worms. As previously characterized, clk-1 mutants have lengthened life spans compared to wild type (Wong et al., 1995). Consistent with this effect, we found transgenic strains with high clk-1 expression to have a shortened average life span. Furthermore, no effect on average life span was found in transgenic control strains in which clk-1 expression was not altered, confirming that the presence of the transgene and the phenotype marking its presence do not influence the observed effects in strains with high clk-1 expression. Together, the findings from both the studies on physiological rates and life span suggest that clk-1 plays a role in determining the rate at which worms age." --
Author | : Barth Demian Grant |
Publisher | : |
Total Pages | : 332 |
Release | : 1996 |
Genre | : |
ISBN | : |
Download Molecular and Genetic Analysis of the Sel-1 Gene of Caenorhabditis Elegans Book in PDF, ePub and Kindle
Author | : Donald L. Riddle |
Publisher | : Firefly Books |
Total Pages | : 1252 |
Release | : 1997 |
Genre | : Medical |
ISBN | : 9780879695323 |
Download C. Elegans II Book in PDF, ePub and Kindle
Defines the current status of research in the genetics, anatomy, and development of the nematode C. elegans, providing a detailed molecular explanation of how development is regulated and how the nervous system specifies varied aspects of behavior. Contains sections on the genome, development, neural networks and behavior, and life history and evolution. Appendices offer genetic nomenclature, a list of laboratory strain and allele designations, skeleton genetic maps, a list of characterized genes, a table of neurotransmitter assignments for specific neurons, and information on codon usage. Includes bandw photos. For researchers in worm studies, as well as the wider community of researchers in cell and molecular biology. Annotation copyrighted by Book News, Inc., Portland, OR
Author | : Kimberly Marie Van Auken |
Publisher | : |
Total Pages | : 346 |
Release | : 1998 |
Genre | : Caenorhabditis elegans |
ISBN | : |
Download Genetic and Molecular Analysis of Nob-1, a Gene Required for Posterior Development in the Caenorhabditis Elegans Embryo Book in PDF, ePub and Kindle