Gene Cloning and Expression Technologies
Author | : Michael P. Weiner |
Publisher | : Biotechniques Press |
Total Pages | : 652 |
Release | : 2002 |
Genre | : Medical |
ISBN | : |
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Author | : Michael P. Weiner |
Publisher | : Biotechniques Press |
Total Pages | : 652 |
Release | : 2002 |
Genre | : Medical |
ISBN | : |
Author | : Sue Carson |
Publisher | : Elsevier |
Total Pages | : 172 |
Release | : 2005-12-15 |
Genre | : Science |
ISBN | : 0080456545 |
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein—students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs*Student-tested labs proven successful in a real classroom laboratories*Exercises simulate a cloning project that would be performed in a real research lab*"Project" approach to experiments gives students an overview of the entire process*Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Author | : David V. Goeddel |
Publisher | : |
Total Pages | : 728 |
Release | : 1990 |
Genre | : Gene Expression |
ISBN | : |
The methods presented in this volume will enable the reader to design effective strategies for the expression of cloned genes and cDNAs and will prove useful in solving the majority of expression problems one is likely to encounter.
Author | : Kakoli Bose |
Publisher | : Springer Nature |
Total Pages | : 315 |
Release | : 2022-01-25 |
Genre | : Medical |
ISBN | : 9811649871 |
This book is immensely useful for graduate students as well as researchers to understand the basics of molecular biology and Recombinant DNA Technology. It provides a comprehensive overview of different approaches for the synthesis of recombinant proteins from E. coli including their cloning, expression and purification. Recent advances in genomics, proteomics, and bioinformatics have facilitated the use of Recombinant DNA Technology for evaluating the biophysical and biochemical properties of various proteins. The book starts with an introductory chapter on gene cloning, protein expression and purification and its implication in current research and commercial applications. Each chapter provides a lucid set of principles, tools and techniques for both students and instructors. The protocols described have been aptly exemplified, and troubleshooting techniques have been included to aid better understanding. Moreover, the set of questions at the end of each chapter have been particularly formulated to help effective learning.
Author | : Siddra Ijaz |
Publisher | : Cambridge Scholars Publishing |
Total Pages | : 156 |
Release | : 2019-08-13 |
Genre | : Science |
ISBN | : 1527538443 |
Genetic engineering is a rapidly growing field in the area of biological sciences. The driving forces behind this are the challenges encountered by health sectors, agriculture, the environment, and industry. As such, accurate and comprehensive knowledge about the philosophy, principles and application of genetic engineering is indispensable for students and researchers to harness maximum opportunities from this field of science. This volume gathers together comprehensive information regarding genetic engineering from recent studies, and presents it in a coherent manner. As such, it will be of interest to undergraduate and postgraduate students and researchers working in the biological sciences.
Author | : Karl Drlica |
Publisher | : John Wiley & Sons |
Total Pages | : 388 |
Release | : 2004 |
Genre | : Science |
ISBN | : |
Publisher's description: With DNA and gene cloning all over the news, readers need to understand the ongoing genetic revolution. In this highly acclaimed guide, Karl Drlica fully explains the basic science and technology readers need to understand the issues and make crucial decisions. Each step of the way he explains complex topics using easy-to-understand analogies.
Author | : Paulina Balbas |
Publisher | : Springer Science & Business Media |
Total Pages | : 505 |
Release | : 2008-02-04 |
Genre | : Science |
ISBN | : 1592597742 |
Since newly created beings are often perceived as either wholly good or bad, the genetic alteration of living cells impacts directly on a symbolic meaning deeply imbedded in every culture. During the earlier years of gene expression research, te- nological applications were confined mainly to academic and industrial laboratories, and were perceived as highly beneficial since molecules that were previously unable to be separated or synthesized became accessible as therapeutic agents. Such were the success stories of hormones, antibodies, and vaccines produced in the bacterium Escherichia coli. Originally this bacterium gained fame among humans for being an unwanted host in the intestine, or worse yet, for being occasionally dangerous and pathogenic. H- ever, it was easily identified in contaminated waters during the 19th century, thus becoming a clear indicator of water pollution by human feces. Tamed, cultivated, and easily maintained in laboratories, its fast growth rate and metabolic capacity to adjust to changing environments fascinated the minds of scientists who studied and modeled such complex phenomena as growth, evolution, genetic exchange, infection, survival, adaptation, and further on—gene expression. Although at the lower end of the complexity scale, this microbe became a very successful model system and a key player in the fantastic revolution kindled by the birth of recombinant DNA technology.
Author | : François Baneyx |
Publisher | : Garland Science |
Total Pages | : 546 |
Release | : 2004 |
Genre | : Medical |
ISBN | : 9780954523251 |
Advances in protein expression technologies have mushroomed in recent years. In this book current and emerging expression technologies are reviewed. Reviews of the molecular genetics of expression systems in various organisms are presented. Topics covered include: Expression of extremophilic proteins; expression in E. coli, Bacillus spp., Saccharomyces cerevisiae, and methylotrophic yeasts; insect cell expression and the baculovirus system; and Chinese Hamster Ovary (CHO) cell lines for large-scale protein production. Also covered are two emerging expression systems, Methylobacterium extorquens AM1 and Caulobacter crescentus.
Author | : Keya Chaudhuri |
Publisher | : The Energy and Resources Institute (TERI) |
Total Pages | : 300 |
Release | : 2013-01-01 |
Genre | : Science |
ISBN | : 8179933202 |
Recombinant DNA Technology is focussed on the current state of knowledge on the recombinant DNA technology and its applications. The book will provide comprehensive knowledge on the principles and concepts of recombinant DNA technology or genetic engineering, protein expression of cloned genes, PCR amplification of DNA, RFLP, AFLP and DNA fingerprinting and finally the most recent siRNA technology. It can be used by post-graduate students studying and teachers teaching in the area of Molecular Biology, Biotechnology, Genetics, Microbiology, Life Science, Pharmacy, Agriculture and Basic Medical Sciences.
Author | : Dr. RAVISHANKAR B.V. |
Publisher | : Lulu.com |
Total Pages | : 214 |
Release | : 2017-09-04 |
Genre | : Education |
ISBN | : 138715592X |
Deoxyribonucleic acid (DNA) is the genetic material of cells. It carries information in a coded form from cell to cell and from parent to offspring. A gene is a linear array of nucleotides located in a particular position on a particular chromosome that encodes a specific functional product (a protein or RNA molecule). When a gene is active, its information is copied first into another nucleic acid, ribonucleic acid (RNA), which in turn directs the synthesis of the gene products, the specific proteins. This lecture introduces some basic concepts of DNA, proteins, genes and genomes.