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Fundamentals of Molecular Diagnostics in Clinical Microbiology

Fundamentals of Molecular Diagnostics in Clinical Microbiology
Author: Dr. Rajdeep Paul
Publisher: DENTOMED PUBLICATION HOUSE
Total Pages: 151
Release: 2024-06-17
Genre: Medical
ISBN: 8119371801

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In the ever-evolving landscape of molecular diagnostics, we find ourselves at a unique intersection of science, technology, and human health. This book embarks on an in-depth exploration of the transformative power of molecular diagnostic technologies, which have revolutionized our understanding of microbial pathogens and their impact on global health. From the dawn of molecular biology to the sophisticated diagnostics of today, the journey has been nothing short of extraordinary. Advances in genomic technologies, such as next-generation sequencing and CRISPR-based diagnostics, have not only enhanced our ability to detect and characterize pathogens but have also paved the way for personalized medicine and precision healthcare. These innovations have provided clinicians with unprecedented tools to diagnose, treat, and manage a myriad of infectious diseases with greater accuracy and efficiency. This comprehensive volume is designed to serve as both a foundational text and forward-looking guide for researchers, clinicians, and policymakers involved in the field of molecular diagnostic microbiology. It into the intricate of pathogen detection, the clinical applications of these technologies, and the ethical, legal, and social implications that accompany their use. The chapters ahead will take you through the principles of nucleic acid extraction, the nuances of bioinformatics in diagnostics, and the critical aspects of quality assurance in laboratory settings. You will also discover the emerging trends and future directions in molecular diagnostics, offering a glimpse into the next frontier of microbial exploration. This book is a testament to the collaborative efforts of scientists, healthcare professionals, and regulatory bodies worldwide, who strive to harness the full potential of molecular diagnostics for the betterment of human health. It is our hope that the insights and knowledge contained within these pages will inspire continued innovation and foster a deeper understanding of the vital role that molecular diagnostics play in modern medicine. We invite you to join us on this journey through the fascinating world of molecular diagnostic microbiology, where each discovery brings us closer to a future where the mysteries of infectious diseases are unraveled, and the promise of personalized medicine is fully realized.


Molecular Diagnostics

Molecular Diagnostics
Author: Lela Buckingham
Publisher:
Total Pages: 0
Release: 2019
Genre: Medical
ISBN: 9780803668294

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Coverage of the application of molecular diagnostics to the clinical laboratory encompass human identification, microbiology, oncology, and tissue typing. More than 300 line drawings clearly illustrate molecular biology concepts and diagnostic applications. "Advanced Concepts" appear in boxes throughout the text to challenge those students interested in learning more. "Historical Highlights" provide a historical perspective to current laboratory practices. "Case Studies" in the clinical chapters illustrate the application of molecular biology concepts to diagnostic testing and patient care. Study Questions at the end of each chapter reinforce the content. Answers, included in the appendix, allow for self assessment outside of the classroom.


Fundamentals of Molecular Diagnostics

Fundamentals of Molecular Diagnostics
Author: David E. Bruns
Publisher: Elsevier Health Sciences
Total Pages: 298
Release: 2007-05-25
Genre: Medical
ISBN: 1416037373

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This book offers an introduction to the newest, fastest-growing field in laboratory science. Explaining and clarifying the molecular techniques used in diagnostic testing, this text provides both entry-level and advanced information. It covers the principles of molecular biology along with genomes and nucleic acid alterations, techniques and instrumentation, and applications of molecular diagnostics. Written by leading experts, including Patrick Bossuyt, Angela Caliendo, Rossa W.K. Chiu, Kojo S.J. Elenitoba-Johnson, Andrea Ferreira-Gonzalez, Amy Groszbach, Sultan Habeebu, Doris Haverstick, Malek Kamoun, Anthony Killeen, Noriko Kusukawa, Y.M. Dennis Lo, Elaine Lyon, Gwendolyn McMillin, Christopher Price, James Versalovic, Cindy Vnencak-Jones, Victor Weedn, Peter Wilding, Thomas Williams, and Carl Wittwer, this book includes illustrations, tables, and a colorful design to make information easy to find and easy to use. A full-color, 4-page insert shows realistic images of the output for many molecular tests. Learning Objectives open each chapter with an overview of what you should achieve. Key Words are listed and defined at the beginning of each chapter, and are bolded in the text. Review Questions at the end of every chapter let you measure your comprehension. Advanced Concepts are included, but set apart from the rest of the text, for students who want a higher level of learning. Ethics boxes address ethical issues, allowing you to apply your knowledge to real-life scenarios. A glossary of all key words may be easily accessed in the back of the book.


Principles and Applications of Molecular Diagnostics

Principles and Applications of Molecular Diagnostics
Author: Nader Rifai
Publisher: Elsevier
Total Pages: 424
Release: 2018-06-13
Genre: Science
ISBN: 0128160624

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Principles and Applications of Molecular Diagnostics serves as a comprehensive guide for clinical laboratory professionals applying molecular technology to clinical diagnosis. The first half of the book covers principles and analytical concepts in molecular diagnostics such as genomes and variants, nucleic acids isolation and amplification methods, and measurement techniques, circulating tumor cells, and plasma DNA; the second half presents clinical applications of molecular diagnostics in genetic disease, infectious disease, hematopoietic malignancies, solid tumors, prenatal diagnosis, pharmacogenetics, and identity testing. A thorough yet succinct guide to using molecular testing technology, Principles and Applications of Molecular Diagnostics is an essential resource for laboratory professionals, biologists, chemists, pharmaceutical and biotech researchers, and manufacturers of molecular diagnostics kits and instruments. Explains the principles and tools of molecular biology Describes standard and state-of-the-art molecular techniques for obtaining qualitative and quantitative results Provides a detailed description of current molecular applications used to solve diagnostics tasks


Molecular Diagnostics

Molecular Diagnostics
Author: Lela Buckingham
Publisher: F. A. Davis Company
Total Pages: 0
Release: 2012
Genre: Medical
ISBN: 9780803626775

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The first text on molecular diagnostics specifically designed for clinical laboratory science programs is back! This exceptional resource introduces the fundamentals of nucleic acid, as well as more advanced concepts. With a focus on the application of molecular concepts in the clinical laboratory to diagnosis diseases, the 2nd Edition includes important updates and improvements to keep up with the rapidly developing field. Inside youll find in-depth explanations of the principles of molecular-based assays as well as reference material, trouble-shooting tips for the laboratory, and discussions that emphasize the continuing emergence of new diagnostic technologies.


Molecular Microbiology

Molecular Microbiology
Author: David H. Persing
Publisher: John Wiley & Sons
Total Pages: 835
Release: 2020-07-24
Genre: Science
ISBN: 1555819079

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Presenting the latest molecular diagnostic techniques in one comprehensive volume The molecular diagnostics landscape has changed dramatically since the last edition of Molecular Microbiology: Diagnostic Principles and Practice in 2011. With the spread of molecular testing and the development of new technologies and their opportunities, laboratory professionals and physicians more than ever need a resource to help them navigate this rapidly evolving field. Editors David Persing and Fred Tenover have brought together a team of experienced researchers and diagnosticians to update this third edition comprehensively, to present the latest developments in molecular diagnostics in the support of clinical care and of basic and clinical research, including next-generation sequencing and whole-genome analysis. These updates are provided in an easy-to-read format and supported by a broad range of practical advice, such as determining the appropriate type and quantity of a specimen, releasing and concentrating the targets, and eliminating inhibitors. Molecular Microbiology: Diagnostic Principles and Practice Presents the latest basic scientific theory underlying molecular diagnostics Offers tested and proven applications of molecular diagnostics for the diagnosis of infectious diseases, including point-of-care testing Illustrates and summarizes key concepts and techniques with detailed figures and tables Discusses emerging technologies, including the use of molecular typing methods for real-time tracking of infectious outbreaks and antibiotic resistance Advises on the latest quality control and quality assurance measures Explores the increasing opportunities and capabilities of information technology Molecular Microbiology: Diagnostic Principles and Practice is a textbook for molecular diagnostics courses that can also be used by anyone involved with diagnostic test selection and interpretation. It is also a useful reference for laboratories and as a continuing education resource for physicians.


PCR for Clinical Microbiology

PCR for Clinical Microbiology
Author: Ian W.J. Carter
Publisher: Springer Science & Business Media
Total Pages: 420
Release: 2010-07-03
Genre: Science
ISBN: 9048190398

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Not another textbook, but a valuable tool for doctors and microbiologists wanting to know how to set up a PCR diagnostic microbiology laboratory according to current regulatory standards and perform assays supplied with patient clinical diagnostic criteria and easy to follow protocols. Whether laboratories are using commercial kits or in-house methods developed in their own laboratories or adopted from published methods, all clinical microbiology laboratories need to be able to understand, critically evaluate, perform and interpret these tests according to rigorous and clinically appropriate standards and international guidelines. The cost and effort of development and evaluation of in-house tests is considerable and many laboratories do not have the resources to do so. This compendium is a vehicle to improve and maintain the clinical relevance and high quality of diagnostic PCR. It is a unique collection of; guidelines for PCR laboratory set up and quality control, test selection criteria, methods and detailed step by step protocols for a diagnostic assays in the field of molecular microbiology. The structure of the book provides the PCR fundamentals and describes the clinical aspects and diagnosis of infectious disease. This is followed by protocols divided into; bacteria, virus, fungi and parasites, and susceptibility screens. The inclusion of medical criteria and interpretation adds value to the compendium and benefits clinicians, scientists, researchers and students of clinical diagnostic microbiology


Molecular Diagnostics

Molecular Diagnostics
Author: Lela Buckingham
Publisher:
Total Pages: 462
Release: 2007
Genre: Molecular diagnosis
ISBN: 9789746520645

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Molecular Diagnostics

Molecular Diagnostics
Author: E. van Pelt-Verkuil
Publisher: Springer
Total Pages: 457
Release: 2019-06-03
Genre: Medical
ISBN: 981131604X

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The books Molecular Diagnostics Part 1 and 2 provide a comprehensive and practical overview of the state-of-the-art molecular biological diagnostic strategies that are being used in a wide variety of disciplines. The editors and experts in their respective fields have combined their knowledge to write these two books. Many years of experience in the development, application and quality control of molecular diagnostic methods is reflected herewith. Molecular Diagnostics Part 1 is dedicated to the theoretical backgrounds of the technologies often applied in molecular diagnostics, in which nucleic acid amplification methods (such as real-time PCR), sequencing and bioinformatics are the basic tools. The assay design and -development, combined with items of trouble-shooting are described in detail. As a foundation of reliable molecular diagnostic assays, the quality control required for validation, implementation and performance of molecular diagnostic assays is thoroughly discussed. This book also provides extensive information for those working with molecular techniques in a wide variety of research applications using conventional and real-time PCR technology, Sanger and high throughput sequencing techniques, and bioinformatics. Molecular Diagnostics Part 2 highlights the applications of the molecular diagnostic methods in the various diagnostic laboratories, comprising: - Clinical microbiology - Clinical chemistry - Clinical genetics - Clinical pathology - Molecular hematopathology - Veterinary health - Plant health - Food safety Both full-colour and well-illustrated books are particularly valuable for students, clinicians, scientists and other professionals who are interested in (designing) molecular diagnostic methods and for those who wish to broaden their knowledge on the current molecular biological revolution. The information in the books highlights the trend of the integration of multiple (clinical) disciplines into one universal molecular laboratory.


Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics 8 E; South Asia Edition;e-Book

Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics 8 E; South Asia Edition;e-Book
Author: Nader Rifai
Publisher: Elsevier India
Total Pages: 1092
Release: 2019-07-16
Genre: Medical
ISBN: 8131258246

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Get the foundational knowledge you need to successfully work in a real-world, clinical lab with Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics, 8th Edition. From highly respected clinical chemistry expert Nader Rifai, this condensed, easier-to-understand version of the acclaimed Tietz Textbook of Clinical Chemistry and Molecular Diagnostics uses a laboratory perspective to guide you through selecting and performing diagnostic lab tests and accurately evaluating the results. Coverage includes laboratory principles, analytical techniques, instrumentation, analytes, pathophysiology, and more. This eighth edition features new clinical cases from The Coakley Collection, new questions from The Deacon's Challenge of Biochemical Calculations Collection, plus new content throughout the text to ensure you stay ahead of all the latest techniques, instrumentation, and technologies. Condensed version of the clinical chemistry "bible" offers the same authoritative and well-presented content in a much more focused and streamlined manner. Coverage of analytical techniques and instrumentation includes optical techniques, electrochemistry, electrophoresis, chromatography, mass spectrometry, enzymology, immunochemical techniques, microchips, automation, and point of care testing. Updated chapters on molecular diagnostics cover the principles of molecular biology, nucleic acid techniques and applications, and genomes and nucleic acid alterations, reflecting the changes in this rapidly evolving field. Learning objectives, key words, and review questions are included in each chapter to support learning. More than 500 illustrations plus easy-to-read tables help readers better understand and remember key concepts