Fret Forster Resonance Energy Transfer PDF Download
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Author | : Igor L. Medintz |
Publisher | : John Wiley & Sons |
Total Pages | : 824 |
Release | : 2013-10-17 |
Genre | : Science |
ISBN | : 3527656049 |
Download FRET - Förster Resonance Energy Transfer Book in PDF, ePub and Kindle
FRET – Förster Resonance Energy Transfer Meeting the need for an up-to-date and detailed primer on all aspects of the topic, this ready reference reflects the incredible expansion in the application of FRET and its derivative techniques over the past decade, especially in the biological sciences. This wide diversity is equally mirrored in the range of expert contributors. The book itself is clearly subdivided into four major sections. The first provides some background, theory, and key concepts, while the second section focuses on some common FRET techniques and applications, such as in vitro sensing and diagnostics, the determination of protein, peptide and other biological structures, as well as cellular biosensing with genetically encoded fluorescent indicators. The third section looks at recent developments, beginning with the use of fluorescent proteins, followed by a review of FRET usage with semiconductor quantum dots, along with an overview of multistep FRET. The text concludes with a detailed and greatly updated series of supporting tables on FRET pairs and Förster distances, together with some outlook and perspectives on FRET. Written for both the FRET novice and for the seasoned user, this is a must-have resource for office and laboratory shelves.
Author | : Theodorus W. J. Gadella |
Publisher | : Elsevier |
Total Pages | : 603 |
Release | : 2011-07-29 |
Genre | : Science |
ISBN | : 0080915124 |
Download FRET and FLIM Techniques Book in PDF, ePub and Kindle
This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.
Author | : Ulrich Kubitscheck |
Publisher | : John Wiley & Sons |
Total Pages | : 508 |
Release | : 2017-03-27 |
Genre | : Medical |
ISBN | : 3527687726 |
Download Fluorescence Microscopy Book in PDF, ePub and Kindle
Zu dem Thema gibt es viele Publikationen, die von Experten für Experten geschrieben wurden. Dieses Buch wendet sich insbesondere an Studenten höherer Semester und Forscher, denen das Hintergrundwissen der Physik fehlt, um neuartige Verfahren der Fluoreszenzmikroskopie zu verstehen. Die zweite Auflage wartet mit neuen Kapiteln und einer erweiterten Einführung auf. Der Schwerpunkt liegt auf der hochauflösenden und Einzelmolekül-Mikroskopie. Jedes Kapitel wurde von einem anerkannten Experten des Fachgebiets geschrieben und sorgfältig überarbeitet, um so die Entwicklungen der letzten Jahre wiederzugeben.
Author | : Joseph R. Lakowicz |
Publisher | : Springer Science & Business Media |
Total Pages | : 487 |
Release | : 2013-11-11 |
Genre | : Science |
ISBN | : 146157658X |
Download Principles of Fluorescence Spectroscopy Book in PDF, ePub and Kindle
Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of the phenomenon of fluorescence. 8 Fluorescence emission occurs about 10- sec (10 nsec) after light absorp tion. During this period of time a wide range of molecular processes can occur, and these can effect the spectral characteristics of the fluorescent compound. This combination of sensitivity and a favorable time scale allows fluorescence methods to be generally useful for studies of proteins and membranes and their interactions with other macromolecules. This book describes the fundamental aspects of fluorescence. and the biochemical applications of this methodology. Each chapter starts with the -theoreticalbasis of each phenomenon of fluorescence, followed by examples which illustrate the use of the phenomenon in the study of biochemical problems. The book contains numerous figures. It is felt that such graphical presentations contribute to pleasurable reading and increased understand ing. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included which illustrate the concepts described in each chapter. Furthermore, a separate chapter is devoted to the instrumentation used in fluorescence spectroscopy. This chapter will be especially valuable for those perform ing or contemplating fluorescence measurements. Such measurements are easily compromised by failure to consider a number of simple principles.
Author | : Hilmi Volkan Demir |
Publisher | : Cambridge University Press |
Total Pages | : 453 |
Release | : 2018-11-22 |
Genre | : Science |
ISBN | : 1107145503 |
Download Applied Nanophotonics Book in PDF, ePub and Kindle
An accessible yet rigorous introduction to nanophotonics, covering basic principles, technology, and applications in lighting, lasers, and photovoltaics. Providing a wealth of information on materials and devices, and over 150 color figures, it is the 'go-to' guide for students in electrical engineering taking courses in nanophotonics.
Author | : Martin Hof |
Publisher | : Springer Science & Business Media |
Total Pages | : 330 |
Release | : 2004-12-08 |
Genre | : Science |
ISBN | : 9783540223382 |
Download Fluorescence Spectroscopy in Biology Book in PDF, ePub and Kindle
Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.
Author | : Igor L. Medintz |
Publisher | : Wiley-VCH |
Total Pages | : 0 |
Release | : 2013-10-17 |
Genre | : Science |
ISBN | : 9783527656035 |
Download FRET - F¿rster Resonance Energy Transfer Book in PDF, ePub and Kindle
FRET – Förster Resonance Energy Transfer Meeting the need for an up-to-date and detailed primer on all aspects of the topic, this ready reference reflects the incredible expansion in the application of FRET and its derivative techniques over the past decade, especially in the biological sciences. This wide diversity is equally mirrored in the range of expert contributors. The book itself is clearly subdivided into four major sections. The first provides some background, theory, and key concepts, while the second section focuses on some common FRET techniques and applications, such as in vitro sensing and diagnostics, the determination of protein, peptide and other biological structures, as well as cellular biosensing with genetically encoded fluorescent indicators. The third section looks at recent developments, beginning with the use of fluorescent proteins, followed by a review of FRET usage with semiconductor quantum dots, along with an overview of multistep FRET. The text concludes with a detailed and greatly updated series of supporting tables on FRET pairs and Förster distances, together with some outlook and perspectives on FRET. Written for both the FRET novice and for the seasoned user, this is a must-have resource for office and laboratory shelves.
Author | : Jeremy Sanderson |
Publisher | : John Wiley & Sons |
Total Pages | : 848 |
Release | : 2019-03-28 |
Genre | : Science |
ISBN | : 1118696816 |
Download Understanding Light Microscopy Book in PDF, ePub and Kindle
Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.
Author | : Yoshiaki Toyama |
Publisher | : Springer Nature |
Total Pages | : 292 |
Release | : 2019-10-02 |
Genre | : Medical |
ISBN | : 9811379084 |
Download Make Life Visible Book in PDF, ePub and Kindle
This open access book describes marked advances in imaging technology that have enabled the visualization of phenomena in ways formerly believed to be completelyimpossible. These technologies have made major contributions to the elucidation of the pathology of diseases as well as to their diagnosis and therapy. The volume presents various studies from molecular imaging to clinical imaging. It also focuses on innovative, creative, advanced research that gives full play to imaging technology inthe broad sense, while exploring cross-disciplinary areas in which individual research fields interact and pursuing the development of new techniques where they fuse together. The book is separated into three parts, the first of which addresses the topic of visualizing and controlling molecules for life. Th e second part is devoted to imaging of disease mechanisms, while the final part comprises studies on the application of imaging technologies to diagnosis and therapy. Th e book contains the proceedings of the 12th Uehara International Symposium 2017, “Make Life Visible” sponsored by the Uehara Memorial Foundation and held from June 12 to 14, 2017. It is written by leading scientists in the field and is an open access publication under a CC BY 4.0 license.
Author | : Hilmi Volkan Demir |
Publisher | : Springer |
Total Pages | : 46 |
Release | : 2016-08-08 |
Genre | : Technology & Engineering |
ISBN | : 9811018766 |
Download Understanding and Modeling Förster-type Resonance Energy Transfer (FRET) Book in PDF, ePub and Kindle
This Brief will focus on the functional uses and applications of FRET, starting with the derivation of FRET in the assemblies of nanostructures and subsequently giving application cases for biologists, physicists, chemists, material scientists, engineers, and those in many other fields whoever would like to FRET as a tool. The goal of this part is therefore to show both specialist and non-specialist how to use and analyze FRET in a wide range of applications.