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Fluorescence Spectroscopy for Cell Membrane Biophysics at the Nanometer and Nanosecond Scales

Fluorescence Spectroscopy for Cell Membrane Biophysics at the Nanometer and Nanosecond Scales
Author: Christopher Randolph Rhodes
Publisher:
Total Pages: 106
Release: 2012
Genre:
ISBN:
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Cell membranes organize and regulate a vital share of biological signaling. Fluorescence has become the main analytical tool with which to study such systems, and novel fluorescence techniques drive biological discovery at ever-smaller size and time scales. By exploiting the unique photophysics of fluorescent probes, I have advanced two parallel approaches to studying membrane systems: nanoengineered biointerfaces and time-resolved spectroscopy. Metallic nanostructures were engineered to precisely localize fluorescence emission, enhance label-free detection of lipid-protein interactions, and manipulate the diffusion landscapes of supported lipid bilayers. A platform for fluorescence spectroscopy at sub-nanosecond resolution combines fluorescence cross-correlation spectroscopy (FCCS) and time-resolved fluorescence anisotropy (TRFA) to characterize protein diffusion. These techniques have provided insight into the organization and structural flexibility of intercellular adhesion molecule-1 (ICAM-1), oligomerization of the small GTPase H-Ras, and the association of diverse lipid anchor domains in vitro and in vivo. Finally, I demonstrate progress toward single-molecule characterization of lipid-protein systems, and elaborate on its relevance to environmental biosensors for public health and biosecurity applications.

Time-Resolved Fluorescence Spectroscopy in Biochemistry and Biology

Time-Resolved Fluorescence Spectroscopy in Biochemistry and Biology
Author: R. Cundall
Publisher: Springer Science & Business Media
Total Pages: 767
Release: 2013-11-11
Genre: Science
ISBN: 1475716346
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At the time that the editors conceived the idea of trying to organize the meeting on which the contents of this volume are based and which became, in March 1980, a NATO Advanced Study Institute, the techniques of time-resolved fluorescence spectroscopy, in both the nanosecond and sub-nanosecond time-domains, might reasonably have been said to be coming of age, both in their execution and in the analysis and interpretation of the results obtained. These techniques, then as now, comprised mainly a number of pulse methods using laser, flash-lamp or, most recently, synchrotron radiation. In addition, significant developments in the more classical phase approach had also rendered that method popular, utilizing either modulation of an otherwise continuous source or, again recently, the ultra-rapid pulse rate attainable with a synchrotron source. In general terms, time-resolved fluorescence studies are capable, under appropriate conditions, of supplying direct kinetic information on both photophysics and various aspects of molecular, macromolecular and supramolecular structure and dynamics. The nanosecond and sub-nanosecond time-scales directly probed render these techniques particularly appropriate in studying relaxation and fluctuation processes in macromolecules, particularly biopolymers (e. g. proteins, nucleic acids), in supramolecular assemblies such as cell membranes, and in a variety of relatively simpler model systems.

Fluorescence Studies on Biological Membranes

Fluorescence Studies on Biological Membranes
Author: Herwig J. Hilderson
Publisher: Springer Science & Business Media
Total Pages: 480
Release: 2012-12-06
Genre: Science
ISBN: 1461393590
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As stated by its first editor, Dr. D. B. Roodyn, the primary goal of the series Subcellular Biochemistry is to achieve an integrated view of the cell by bringing together results from a wide range of different techniques and disciplines. This volume deals with the applications of fluorescence spectroscopy to membrane research. It seeks to present complementary biochemical and bio physical data on both the structure and the dynamics of biological membranes. Biophysics and biochemistry are improving more and more in their ability to study biomembranes, overlapping somewhat in this area and explaining the functioning of the whole cell in terms of the properties of its individual com ponents. Therefore, we have brought together an international group of experts in order to report on and review advances in fluorescence studies on biological membranes, thereby highlighting subcellular aspects. The first chapters present a critical evaluation of the current applications of dynamic and steady-state fluorescence techniques. Subsequent chapters dis cuss more specific applications in cells, biological membranes, and their con stituents (lipids, proteins).

Biochemical Applications

Biochemical Applications
Author: Joseph R. Lakowicz
Publisher: Springer Science & Business Media
Total Pages: 398
Release: 1992-02-29
Genre: Science
ISBN: 0306439549
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Fluorescence spectroscopy and its applications to the physical and life sciences have evolved rapidly during the past decade. The increased interest in fluorescence appears to be due to advances in time resolution, methods of data analysis and improved instrumentation. With these advances, it is now practical to perform time-resolved measurements with enough resolution to compare the results with the structural and dynamic features of mac- molecules, to probe the structures of proteins, membranes, and nucleic acids, and to acquire two-dimensional microscopic images of chemical or protein distributions in cell cultures. Advances in laser and detector technology have also resulted in renewed interest in fluorescence for clinical and analytical chemistry. Because of these numerous developments and the rapid appearance of new methods, it has become difficult to remain current on the science of fluorescence and its many applications. Consequently, I have asked the experts in particular areas of fluorescence to summarize their knowledge and the current state of the art. This has resulted in the initial three volumes of Topics in Fluorescence Spectroscopy, which is intended to be an ongoing series which summarizes, in one location, the vast literature on fluorescence spectroscopy. These first three volumes are designed to serve as an advanced text. These volumes describe the more recent techniques and technologies (Volume 1), the principles governing fluorescence and the experimental observables (Volume 2), and applications in biochemistry and biophysics (Volume 3).

Fluorescence Spectroscopy and Microscopy in Biology

Fluorescence Spectroscopy and Microscopy in Biology
Author: Radek Šachl
Publisher: Springer Nature
Total Pages: 532
Release: 2023-04-27
Genre: Science
ISBN: 3031303628
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This book provides the reader with an updated comprehensive view of the rapidly developing and fascinating field of fluorescence spectroscopy and microscopy. In recent years, fluorescence spectroscopy and microscopy have experienced rapid technological development, which has enabled the detection and monitoring of single molecules with high spatial and temporal resolution. Thanks to these developments, fluorescence has become an even more popular method in physical, biological and related fields. This book guides the reader through both basic and advanced fluorescence spectroscopy and microscopy approaches with a focus on their applications in membrane and protein biophysics. Each of the four parts: A - Fluorescence Spectroscopy, B - Fluorescence Microscopy, C - Applications of Fluorescence Spectroscopy and Microscopy to biological membranes and D - Applications of Fluorescence Spectroscopy to protein studies are written by experts within the field. The book is intended for both complete beginners who want to quickly orient themselves in the large number of existing fluorescent methods, as well as for advanced readers who are interested in particular methods and their proper use.

Fluorescence Spectroscopy in Biology

Fluorescence Spectroscopy in Biology
Author: Martin Hof
Publisher: Springer
Total Pages: 0
Release: 2010-10-21
Genre: Science
ISBN: 9783642061066
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Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.

Biophysical and Biochemical Aspects of Fluorescence Spectroscopy

Biophysical and Biochemical Aspects of Fluorescence Spectroscopy
Author: T.G. Dewey
Publisher: Springer
Total Pages: 294
Release: 1991-04-30
Genre: Science
ISBN: 0306436272
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Fluorescence spectroscopy has traditionally found wide application in bio chemistry and cell biology. Since there are relatively few naturally occurring fluorescent biomolecules, fluorescence spectroscopy offers a combination of great specificity and sensitivity. Historically, these features have been ex ploited with great success utilizing both intrinsic and extrinsic probes. Re cent applications have built upon these traditional strengths and have re sulted in the development of new instrumental techniques, novel and convenient fluorescent probes, and a deeper, theoretical understanding of fundamental processes. Frequently, fluorescence techniques are tailored to attack a specific biological problem. These new methods in turn produce new physical situations and phenomena which are often of interest to the physical chemist. Thus, progress in one area stimulates renewed interest in other areas. The goal of this book is to provide detailed monographs on the use of fluorescence to investigate problems at the forefront of biochemistry and cell biology. This book is not meant to be a comprehensive survey but rather to highlight areas of recent developments. It is designed to be readable to the novice and yet provide sufficient detail for the expert to keep abreast of recent developments. The book is organized so that it proceeds from simple biochemical sys tems to more complex cell biological ones. Chapter I on fluorescence quenching of biological structures is a good introductory chapter. It intro duces a number of elementary concepts and discusses applications to pro teins and biomembranes.

Biophysics

Biophysics
Author: Mark C. Leake
Publisher: CRC Press
Total Pages: 457
Release: 2023-12-12
Genre: Medical
ISBN: 100380652X
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Biophysics: Tools and Techniques for the Physics of Life covers the experimental, theoretical, and computational tools and techniques of biophysics. It addresses the purpose, science, and application of all physical science instrumentation, theoretical analysis, and biophysical computational methods used in current research labs. The book first presents the historical background, concepts, and motivation for using a physical science toolbox to understand biology. It then familiarizes undergraduate students from the physical sciences with essential biological knowledge. The text subsequently focuses on experimental biophysical techniques that primarily detect biological components or measure/control biological forces. The author describes the science and application of key tools used in imaging, detection, general quantitation, and biomolecular interaction studies, which span multiple length and time scales of biological processes both in the test tube and in the living organism. Moving on to theoretical and computational biophysics tools, the book presents analytical mathematical methods and numerical simulation approaches for tackling challenging biological questions including exam-style questions at the end of each chapter as well as step-by-step solved exercises. It concludes with a discussion of the future of this exciting field. Future innovators will need to be trained in multidisciplinary science to be successful in industry, academia, and government support agencies. Addressing this challenge, this textbook educates future leaders on the development and application of novel physical science approaches to solve complex problems linked to biological questions. Features: Provides the full, modern physical science toolbox of experimental, theoretical, and computational techniques, such as bulk ensemble methods, single-molecule tools, live-cell and test tube methods, pencil-on-paper theory approaches, and simulations. Incorporates worked examples for the most popular physical science tools by providing full diagrams and a summary of the science involved in the application of the tool. Reinforces the understanding of key concepts and biological questions. A solutions manual is available upon qualifying course adoption.

The Biophysics of Cell Membranes

The Biophysics of Cell Membranes
Author: Richard M. Epand
Publisher: Springer
Total Pages: 224
Release: 2017-09-25
Genre: Science
ISBN: 9811062447
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This volume focuses on the modulation of biological membranes by specific biophysical properties. The readers are introduced to emerging biophysical approaches that mimick specific states (like membrane lipid asymmetry, membrane curvature, lipid flip-flop, lipid phase separation) that are relevant to the functioning of biological membranes. The first chapter describes innovative methods to mimic the prevailing asymmetry in biological membranes by forming asymmetrical membranes made of monolayers with different compositions. One of the chapters illustrates how physical parameters, like curvature and elasticity, can affect and modulate the interactions between lipids and proteins. This volume also describes the sensitivity of certain ion channels to mechanical forces and it presents an analysis of how cell shape is determined by both the cytoskeleton and the lipid domains in the membrane. The last chapter provides evidence that liposomes can be used as a minimal cellular model to reconstitute processes related to the origin of life. Each topic covered in this volume is presented by leading experts in the field who are able to present clear, authoritative and up-to-date reviews. The novelty of the methods proposed and their potential for a deeper molecular description of membrane functioning are particularly relevant experts in the areas of biochemistry, biophysics and cell biology, while also presenting clear and thorough introductions, making the material suitable for students in these fields as well.