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Fluorescence Applications in Biotechnology and Life Sciences

Fluorescence Applications in Biotechnology and Life Sciences
Author: Ewa M. Goldys
Publisher: John Wiley & Sons
Total Pages: 398
Release: 2009-08-24
Genre: Science
ISBN: 0470083700

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A self-contained treatment of the latest fluorescence applications in biotechnology and the life sciences This book focuses specifically on the present applications of fluorescence in molecular and cellular dynamics, biological/medical imaging, proteomics, genomics, and flow cytometry. It raises awareness of the latest scientific approaches and technologies that may help resolve problems relevant for the industry and the community in areas such as public health, food safety, and environmental monitoring. Following an introductory chapter on the basics of fluorescence, the book covers: labeling of cells with fluorescent dyes; genetically encoded fluorescent proteins; nanoparticle fluorescence probes; quantitative analysis of fluorescent images; spectral imaging and unmixing; correlation of light with electron microscopy; fluorescence resonance energy transfer and applications; monitoring molecular dynamics in live cells using fluorescence photo-bleaching; time-resolved fluorescence in microscopy; fluorescence correlation spectroscopy; flow cytometry; fluorescence in diagnostic imaging; fluorescence in clinical diagnoses; immunochemical detection of analytes by using fluorescence; membrane organization; and probing the kinetics of ion pumps via voltage-sensitive fluorescent dyes. With its multidisciplinary approach and excellent balance of research and diagnostic topics, this book is an essential resource for postgraduate students and a broad range of scientists and researchers in biology, physics, chemistry, biotechnology, bioengineering, and medicine.


Fluorescence Spectroscopy in Biology

Fluorescence Spectroscopy in Biology
Author: Martin Hof
Publisher: Springer Science & Business Media
Total Pages: 315
Release: 2006-01-27
Genre: Science
ISBN: 3540270043

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Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.


Fluorescence Microscopy in Life Sciences

Fluorescence Microscopy in Life Sciences
Author: Juan Carlos Stockert
Publisher: Bentham Science Publishers
Total Pages: 751
Release: 2017-12-15
Genre: Science
ISBN: 1681085186

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Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained.


New Trends in Fluorescence Spectroscopy

New Trends in Fluorescence Spectroscopy
Author: Bernard Valeur
Publisher: Springer Science & Business Media
Total Pages: 496
Release: 2012-12-06
Genre: Science
ISBN: 364256853X

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This first volume in the new Springer Series on Fluorescence brings together fundamental and applied research from this highly interdisciplinary and field, ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of articles is an invaluable tool for practitioners and novices.


The Future of Dynamic Structural Science

The Future of Dynamic Structural Science
Author: Judith A.K. Howard
Publisher: Springer
Total Pages: 292
Release: 2014-07-08
Genre: Science
ISBN: 9401785503

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This work focuses on complementary crystallographic and spectroscopic areas of dynamic structural science, from papers presented at the 46th NATO sponsored course in Erice, Sicily 2013. These papers cover a range of material from background concepts to more advanced material and represent a fully inter-disciplinary collection of the latest ideas and results within the field. They will appeal to practising or novice crystallographers, both chemical and biological, who wish to learn more about modern spectroscopic methods and convergent advances and hence vice versa for experimental and computational spectroscopists. The chapters refer to the latest techniques, software and results and each chapter is fully referenced. The volume provides an excellent starting point for new comers in the emerging, multi-disciplinary area of time resolved science.


Forensic Microscopy

Forensic Microscopy
Author: Ritesh K Shukla
Publisher: CRC Press
Total Pages: 367
Release: 2022-07-12
Genre: Science
ISBN: 1000600149

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Forensic Microscopy: Truth Under the Lenses provides an overview and understanding of the various types of microscopes and their techniques employed in forensic science. The book emphasizes both the theoretical and practical aspects of microscopy to enrich the reader’s understanding of the various tools, techniques, and utility—including strengths and weaknesses—of types of microscopes in analyzing certain forms of evidence. The book begins with the history of microscopes, the basic optics for microscopy, then moves to advanced microscopies such as electron microscopes and atomic force microscopes. In addition to the various types of microscopes and how to use and best utilize them, the book looks at the analysis of specific types of evidence, including hair, fiber, fingerprint, body fluids, tool marks, ink, pollen grains, spores, diatoms, bullets, cartridges, among other evidence types. Since forensic science is an applied, hands-on discipline, the book includes both a theoretical and a practical approach to the topic. Key Features: Addresses simple to advanced microscopy techniques for the effective analyses of trace evidence Pairs chapters on a particular type of microscopy, explaining it thoroughly, before delving into specific usage for forensic applications Presents theories and as well as real-world application of concepts Provides abundant micro-photographs, including graphical representations and flow charts, to illustrate concepts clearly Forensic Microscopy serves as a helpful reference for undergraduate and postgraduate students in forensic science, forensic biology, forensic chemistry and related programs. It is also recommended for research students, academicians, technicians, industry and laboratory professionals working on trace evidence analysis.


Fluorescent Proteins I

Fluorescent Proteins I
Author: Gregor Jung
Publisher: Springer Science & Business Media
Total Pages: 275
Release: 2011-10-25
Genre: Science
ISBN: 3642233724

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Fluorescent proteins are intimately connected to research in the life sciences. Tagging of gene products with fluorescent proteins has revolutionized all areas of biosciences, ranging from fundamental biochemistry to clinical oncology, to environmental research. The discovery of the Green Fluorescent Protein, its first, seminal application and the ingenious development of a broad palette of fluorescence proteins of other colours, was consequently recognised with the Nobel Prize for Chemistry in 2008. Fluorescent Proteins I is devoted to the basic photophysical and photochemical aspects of fluorescent protein technology. Experienced experts highlight colour tuning, the exploration of switching phenomena and respective methods for their investigation. The book provides a thorough understanding of primary molecular processes allowing the design of fluorescent proteins for specific applications.


Molecular Fluorescence

Molecular Fluorescence
Author: Bernard Valeur
Publisher: John Wiley & Sons
Total Pages: 595
Release: 2013-03-25
Genre: Science
ISBN: 3527328467

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This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most significant developments. The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.


Advanced Time-Correlated Single Photon Counting Applications

Advanced Time-Correlated Single Photon Counting Applications
Author: Wolfgang Becker
Publisher: Springer
Total Pages: 624
Release: 2015-04-13
Genre: Science
ISBN: 3319149296

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This book is an attempt to bridge the gap between the instrumental principles of multi-dimensional time-correlated single photon counting (TCSPC) and typical applications of the technique. Written by an originator of the technique and by sucessful users, it covers the basic principles of the technique, its interaction with optical imaging methods and its application to a wide range of experimental tasks in life sciences and clinical research. The book is recommended for all users of time-resolved detection techniques in biology, bio-chemistry, spectroscopy of live systems, live cell microscopy, clinical imaging, spectroscopy of single molecules, and other applications that require the detection of low-level light signals at single-photon sensitivity and picosecond time resolution.


Fluorescent Proteins II

Fluorescent Proteins II
Author: Gregor Jung
Publisher: Springer Science & Business Media
Total Pages: 287
Release: 2012-01-05
Genre: Science
ISBN: 3642233767

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Fluorescent proteins are intimately connected to research in the life sciences. Tagging of gene products with fluorescent proteins has revolutionized all areas of biosciences, ranging from fundamental biochemistry to clinical oncology, to environmental research. The discovery of the Green Fluorescent Protein, its first, seminal application and the ingenious development of a broad palette of fluorescence proteins of other colours, was consequently recognised with the Nobel Prize for Chemistry in 2008. Fluorescent Proteins II highlights the physicochemical and biophysical aspects of fluorescent protein technology beyond imaging. It is tailored to meet the needs of physicists, chemists and biologists who are interested in the fundamental properties of fluorescent proteins, while also focussing on specific applications. The implementations described are cutting-edge studies and exemplify how the physical and chemical properties of fluorescent proteins can stimulate novel findings in life sciences.