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Far-Field Optical Nanoscopy

Far-Field Optical Nanoscopy
Author: Philip Tinnefeld
Publisher: Springer
Total Pages: 340
Release: 2015-02-07
Genre: Science
ISBN: 3662455471

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This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.


Near Field Optics and Nanoscopy

Near Field Optics and Nanoscopy
Author: J. P. Fillard
Publisher: World Scientific
Total Pages: 470
Release: 1996
Genre: Science
ISBN: 9789810223496

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This book contains the most recent information on optical nanoscopy. Far-Field and Near-Field properties on e.m. waves are presented which illustrate how optical images can be obtained from sub-micron objects. Scanning Probe techniques and computer processing are covered here. An explanation is given on how propagating photons or evanescent waves can behave over distances shorter than the wavelength, taking into account the presence of small objects. Quantum tunneling of photons is explained comparatively with the electron mechanism. Technical details are given on photon tunneling microscopes. Typical results already obtained with these techniques are also described.


Single Molecule Spectroscopy in Chemistry, Physics and Biology

Single Molecule Spectroscopy in Chemistry, Physics and Biology
Author: Astrid Graslund
Publisher: Springer Science & Business Media
Total Pages: 570
Release: 2010-04-17
Genre: Molecular spectroscopy
ISBN: 3642025986

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Written by the leading experts in the field, this book describes the development and current state of the art in single molecule spectroscopy. The application of this technique, which started 1989, in physics, chemistry and biosciences is displayed.


Nanoscale Photonic Imaging

Nanoscale Photonic Imaging
Author: Tim Salditt
Publisher: Springer Nature
Total Pages: 634
Release: 2020-06-09
Genre: Science
ISBN: 3030344134

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This open access book, edited and authored by a team of world-leading researchers, provides a broad overview of advanced photonic methods for nanoscale visualization, as well as describing a range of fascinating in-depth studies. Introductory chapters cover the most relevant physics and basic methods that young researchers need to master in order to work effectively in the field of nanoscale photonic imaging, from physical first principles, to instrumentation, to mathematical foundations of imaging and data analysis. Subsequent chapters demonstrate how these cutting edge methods are applied to a variety of systems, including complex fluids and biomolecular systems, for visualizing their structure and dynamics, in space and on timescales extending over many orders of magnitude down to the femtosecond range. Progress in nanoscale photonic imaging in Göttingen has been the sum total of more than a decade of work by a wide range of scientists and mathematicians across disciplines, working together in a vibrant collaboration of a kind rarely matched. This volume presents the highlights of their research achievements and serves as a record of the unique and remarkable constellation of contributors, as well as looking ahead at the future prospects in this field. It will serve not only as a useful reference for experienced researchers but also as a valuable point of entry for newcomers.


High Resolution Imaging in Microscopy and Ophthalmology

High Resolution Imaging in Microscopy and Ophthalmology
Author: Josef F. Bille
Publisher: Springer
Total Pages: 407
Release: 2019-08-13
Genre: Medical
ISBN: 3030166384

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This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.


Coordinate-targeted Optical Nanoscopy: Molecular Photobleaching and Imaging of Heterostructured Nanowires

Coordinate-targeted Optical Nanoscopy: Molecular Photobleaching and Imaging of Heterostructured Nanowires
Author: Joanna Oracz
Publisher:
Total Pages: 0
Release: 2018
Genre:
ISBN:

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Super-resolution far-field fluorescence microscopy (optical nanoscopy) is a mature set of methods which enable visualization of the nanometer-scale distribution of objects such as organic molecules, photoswitchable proteins, point-like defects in the diamond lattice, upconversion nanoparticles, semiconductor quantum dots, etc. Utilization of internal emitter states in the imaging scheme has made it possible to create contrast at the nanoscale in conventional lens-based optical imaging systems. Spatial resolutions down to the single nanometer scale have been reported in some cases. Nonethele...


Nanoscopy and Nanospectroscopy

Nanoscopy and Nanospectroscopy
Author: Sandip Dhara
Publisher: CRC Press
Total Pages: 436
Release: 2023-03-31
Genre: Science
ISBN: 1000845818

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This book builds a narrative on the near-field optical and spectroscopic studies with an emphasis on plasmonic- and photonic-assisted nano-optics as a tool for superlensing. Deliberations on near-field studies using confined light in various applications are included along with their commercial implications. Single-molecule detection utilizing efficient surface-enhanced Raman scattering phenomenon in the far-field and plasmonic tip-enhanced Raman scattering studies in the near-field measurements for fast analysis up to trace level is discussed. Features: Covers the broad area of nano-optical spectroscopy from the perspective of putting the concepts and innovations in the field to use. Discusses entire spectra of near-field optics and spectroscopy using light. Explores gas/chemical sensing using surface plasmon resonance (SPR) in the Kretschmann configuration. Includes dielectric nano-photonics and optical confinement. Studies phonon behaviour using confined light for the analysis of chemical, biological, and other materials. This book is aimed at graduate students and researchers in material science, analytical chemistry, nanotechnology, and electrical engineering.


Nano-optics and Near-field Optical Microscopy

Nano-optics and Near-field Optical Microscopy
Author: Anatoly V. Zayats
Publisher: Artech House
Total Pages: 379
Release: 2009
Genre: Science
ISBN: 1596932848

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"This groundbreaking book focuses on near-field microscopy which has opened up optical processes at the nanoscale for direct inspection. Further, it explores the emerging area of nano-optics which promises to make possible optical microscopy with true nanometer resolution. This frontline resource helps you achieve high resolution optical imaging of biological species and functional materials. You also find guidance in the imaging of optical device operation and new nanophotonics functionalities"--EBL.


Optical Nanoscopy and Novel Microscopy Techniques

Optical Nanoscopy and Novel Microscopy Techniques
Author: Peng Xi
Publisher: CRC Press
Total Pages: 268
Release: 2014-09-19
Genre: Science
ISBN: 1466586303

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Microscopy is at the forefront of multidisciplinary research. It was developed by physicists, made specific by chemists, and applied by biologists and doctors to better understand how the human body works. For this very reason, the field has been revolutionized in past decades.The objective of Optical Nanoscopy and Novel Microscopy Techniques is to


Label-Free Super-Resolution Microscopy

Label-Free Super-Resolution Microscopy
Author: Vasily Astratov
Publisher: Springer Nature
Total Pages: 487
Release: 2019-08-31
Genre: Science
ISBN: 3030217221

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This book presents the advances in super-resolution microscopy in physics and biomedical optics for nanoscale imaging. In the last decade, super-resolved fluorescence imaging has opened new horizons in improving the resolution of optical microscopes far beyond the classical diffraction limit, leading to the Nobel Prize in Chemistry in 2014. This book represents the first comprehensive review of a different type of super-resolved microscopy, which does not rely on using fluorescent markers. Such label-free super-resolution microscopy enables potentially even broader applications in life sciences and nanoscale imaging, but is much more challenging and it is based on different physical concepts and approaches. A unique feature of this book is that it combines insights into mechanisms of label-free super-resolution with a vast range of applications from fast imaging of living cells to inorganic nanostructures. This book can be used by researchers in biological and medical physics. Due to its logically organizational structure, it can be also used as a teaching tool in graduate and upper-division undergraduate-level courses devoted to super-resolved microscopy, nanoscale imaging, microscopy instrumentation, and biomedical imaging.