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Extracellular Polymeric Substances - the Construction Material of Biofilms

Extracellular Polymeric Substances - the Construction Material of Biofilms
Author: Hans-Curt Flemming
Publisher: IWA Publishing (International Water Assoc)
Total Pages: 220
Release: 2001-03-31
Genre: Science
ISBN:

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Microbial extracellular polymeric substances (EPS) provide the structural matrix for all microbial aggregates such as biofilms, flocs and sludge, and are of great technical and ecological importance. EPS provide and form the space between biofilm cells, mediating both cohesion and adhesion. They serve as a multipurpose functional matrix for microbial communities, which allows the establishment of stable microconsortia. The EPS matrix governs diffusional and convectional mass transport because it occurs in various densities, including void spaces such as pores and channels. This creates different types of physico-chemical gradients, providing habitats in which aerobic and anaerobic organisms can coexist in close proximity and largely independent of the conditions in the water phase. Major aspects of EPS include mechanical stability, as in the case of biofouling when unwanted biofilms have to be removed, and the stability of biofilms in bioreactors; interaction with substrata in bioleaching, biocorrosion, and biodeterioration; sorption capacity for dissolved and particulate substances (biosorption), representing both a sink and source of sorbed material; enhanced resistance of biofilm organisms to biocides. The 25 peer-reviewed articles selected for these proceedings provde both a starting point for newcomers to the field and a state-of-the-art survey for the more experienced. With a diversity of approaches, ranging from sophisticated microanalysis to industrial applications, these papers provide a broad understanding of EPS from the viewpoints of microbiologists, polymer scientists, chemists and engineers, and showing how EPS may be seen as the key to understanding and controlling all forms of microbial aggregates.


Microbial Extracellular Polymeric Substances

Microbial Extracellular Polymeric Substances
Author: Jost Wingender
Publisher: Springer Science & Business Media
Total Pages: 266
Release: 2012-12-06
Genre: Science
ISBN: 3642601472

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Microbial extracellular polymeric substances (EPS) are the key components for the aggregation of microorganisms in biofilms, flocs and sludge. They are composed of polysaccharides, proteins, nucleic acids, lipids and other biological macromolecules. EPS provide a highly hydrated gel matrix in which microbial cells can establish stable synergistic consortia. Cohesion and adhesion as well as morphology, structure, biological function and other properties such as mechanical stability, diffusion, sorption and optical properties of microbial aggregates are determined by the EPS matrix. Also, the protection of biofilm organisms against biocides is attributed to the EPS. Their matrix allows phase separation in biofiltration and is also important for the degradation of particulate material which is of great importance for the self purification processes in surface waters and for waste water treatment.


The Perfect Slime

The Perfect Slime
Author: Hans-Curt Flemming
Publisher: IWA Publishing
Total Pages: 336
Release: 2016-09-15
Genre: Science
ISBN: 1780407416

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The Perfect Slime presents the latest state of knowledge and all aspects of the Extracellular Polymeric Substances, (EPS) matrix – from the ecological and health to the antifouling perspectives. The book brings together all the current material in order to expand our understanding of the functions, properties and characteristics of the matrix as well as the possibilities to strengthen or weaken it. The EPS matrix represents the immediate environment in which biofilm organisms live. From their point of view, this matrix has paramount advantages. It allows them to stay together for extended periods and form synergistic microconsortia, it retains extracellular enzymes and turns the matrix into an external digestion system and it is a universal recycling yard, it protects them against desiccation, it allows for intense communication and represents a huge genetic archive. They can remodel their matrix, break free and eventually, they can use it as a nutrient source. The EPS matrix can be considered as one of the emergent properties of biofilms and are a major reason for the success of this form of life. Nevertheless, they have been termed the “black matter of biofilms” for good reasons. First of all: the isolation methods define the results. In most cases, only water soluble EPS components are investigated; insoluble ones such as cellulose or amyloids are much less included. In particular in environmental biofilms with many species, it is difficult to impossible isolate, separate the various EPS molecules they are encased in and to define which species produced which EPS. The regulation and the factors which trigger or inhibit EPS production are still very poorly understood. Furthermore: bacteria are not the only microorganisms to produce EPS. Archaea, Fungi and algae can also form EPS. This book investigates the questions, What is their composition, function, dynamics and regulation? What do they all have in common?


Community Structure and Co-operation in Biofilms

Community Structure and Co-operation in Biofilms
Author: Society for General Microbiology. Symposium
Publisher: Cambridge University Press
Total Pages: 366
Release: 2000-10-23
Genre: Medical
ISBN: 9780521793025

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The study of biofilm considers the close association of micro-organisms with each other at interfaces and is relevant to a variety of disciplines, including medicine, dentistry, bioremediation, biofouling, water technology, engineering and food science. Although the habitats studied differ widely, some common elements exist such as method of attachment, coadhesion and regulation of biofilm phenotype and architecture. This book aims to distil the common principles of biofilm physiology and growth for all interested disciplines.


Biofilms

Biofilms
Author: L V Evans
Publisher: CRC Press
Total Pages: 507
Release: 2003-09-02
Genre: Science
ISBN: 0203304721

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Biofilms affect the lives of all of us, growing as they do for example on our teeth (as plaque), on catheters and medical implants in our bodies, on our boats and ships, in food processing environments, and in drinking and industrial water treatment systems. They are highly complex biological communities whose detailed structure and functioning is only gradually being unravelled, with the development of increasingly sophisticated technology for their study. Biofilms almost always have a negative impact on human affairs (flocs in sewage treatment plants are a major exception) and a lot of research is being carried out to gain a better understanding of them, so that we will be in a better position to control them. This volume, with contributions by international experts from widely diverse areas of this field, presents a state-of-the-art picture of where we are at present in terms of our knowledge of biofilms, the techniques being used to study them, and possible strategies for controlling their growth more successfully. It should provide a valuable reference source for information on biofilms and their control for many years to come.


Microbial Extracellular Polymeric Substances

Microbial Extracellular Polymeric Substances
Author: Jost Wingender
Publisher:
Total Pages: 276
Release: 1999-10-20
Genre:
ISBN: 9783642601484

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Microbial extracellular polymeric substances (EPS) are the key components for the aggregation of microorganisms in biofilms, flocs and sludge. They are composed of polysaccharides, proteins, nucleic acids, lipids and other biological macromolecules. EPS provide a highly hydrated gel matrix in which microbial cells can establish stable synergistic consortia. Cohesion and adhesion as well as morphology, structure, biological function and other properties such as mechanical stability, diffusion, sorption and optical properties of microbial aggregates are determined by the EPS matrix. Also, the protection of biofilm organisms against biocides is attributed to the EPS. Their matrix allows phase separation in biofiltration and is also important for the degradation of particulate material which is of great importance for the self purification processes in surface waters and for waste water treatment. In this volume, analysis, characterization, composition, regulation, function and interactions of microbial EPS are covered.


Oral Biofilms

Oral Biofilms
Author: S. Eick
Publisher: Karger Medical and Scientific Publishers
Total Pages: 247
Release: 2020-12-21
Genre: Medical
ISBN: 3318068527

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Biofilms are highly organized polymicrobial communities that are embedded in an extracellular matrix and formed on natural and artificial surfaces. In the oral cavity, biofilms are formed not only on natural teeth, but also on restorative materials, prosthetic constructions, and dental implants. Oral diseases like caries, gingivitis, periodontitis, and also pulp inflammation are associated with biofilms. This publication is an up-to-date overview on oral biofilms from different clinically relevant perspectives. Experts comprising basic researchers and clinicians report on recent research relating to biofilms - from general summaries to recommendations for daily clinical work. This book covers all aspects of oral biofilms, including models used in the laboratory, biofilms in dental water unit lines, periodontal and peri-implant biofilms, caries-related biofilms, halitosis, endodontic biofilms, and Candida infections, as well as biofilms on dental materials and on orthodontic appliances. Several chapters deal with anti-biofilm therapy, from the efficacy of mechanical methods and the use of antimicrobials, to alternative concepts. This publication is particularly recommended to dental medicine students, practitioners, other oral healthcare professionals, and scientists with an interest in translational research on biofilms.


Sediment Dynamics and Pollutant Mobility in Rivers

Sediment Dynamics and Pollutant Mobility in Rivers
Author: Bernd Westrich
Publisher: Springer Science & Business Media
Total Pages: 463
Release: 2007-10-11
Genre: Science
ISBN: 3540347852

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This is the first interdisciplinary book on the mobilization of nutrients and pollutants in the water phase due to hydrodynamic processes. Coverage includes the formation of aggregates in turbulent water; flocks and biofilms from organic reactions; and the formation of new surfaces for re-adsorption of dissolved pollutants. The book gathers papers resulting from an International Symposium on Sediment Dynamics and Pollutant Mobility in River Basins in Hamburg, Germany, March, 2006.


The MBR Book

The MBR Book
Author: Simon Judd
Publisher: Elsevier
Total Pages: 537
Release: 2010-11-26
Genre: Technology & Engineering
ISBN: 0080967671

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The MBR Book covers all essential aspects of membrane bioreactors in water and wastewater treatment, including the working principles of MBR technologies. The book aims to separate science from engineering, in an attempt to avoid confusion and to help readers understand the ideas of MBR. The text is divided into five chapters; the membrane and biological aspects are discussed in chapter 2 along with scientific studies. The third chapter covers the design, operation, and maintenance of MBR, including cost modeling and cost benefit analysis. Chapters 4 and 5 cover the commercial MBR products and their applications for water and wastewater treatment, respectively. The text features industrial case studies, along with useful appendices of commercial and international membrane organizations. The book serves as a a primary reference for chemical, environmental, and process engineers, as well as environmental researchers, natural resources researchers, filtration specialists, water company managers, and consultants. Membrane Bioreactors are a major growth area in the water and waste water treatment industries Internationally-known author, one of the leading senior experts in MBR research Principles and practice, backed by industrial case studies


Advances in Biofilm Science and Engineering

Advances in Biofilm Science and Engineering
Author: Ryan Jordan
Publisher: Cytergy
Total Pages: 282
Release: 2004
Genre: Biofilms
ISBN: 0974180211

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Microbial biofilms provide challenges in many fields of science, technology, and engineering. Advances in Biofilm Science and Engineering offers a state of the art review of new science about microbial biofilms in the areas of biotechnology, healthcare, and medicine.The purpose of this text is to strengthen [the biofilm] information nucleus by offering six core discussions of established concepts in biofilm science. The audience for this text is not just academic; it is trade. This book is for physicians, industrial microbiologists, product managers, process engineers, policy makers and public health officials. [The] authors represent some of the brightest researchers in the field of biofilm science and engineering and comprise a small subset of the research community with a passion for bringing biofilm information to industrial and medical trade professionals.Table of ContentsForward, by Ryan JordanChapter 1. The History and Use of Heterotrophic Plate Counts in Water Systems, by Anne Camper (Center for Biofilm Engineering, Montana State University)Chapter 2. Methods for Biofilm Study and Assays for Biofilm Susceptibility and Target Recognition: Approaches to Deal with the Biofilm Mode of Life, by Howard Ceri, Merle E. Olson, D. Storey, and D. Morck (Biofilm Research Group and Biological Sciences, University of Calgary)Chapter 3. Extracellular Polymeric Substances, by H.C. Flemming and J. Wingender (Gerhard-Mercator-University Duisburg Insitute of Interfacial Biotechnology Department of Aquatic Microbiology, Duisburg, Germany)Chapter 4. Biofilms and Antimicrobial Resistance: Beyond Physiological and Chemical Elements, by Peter Gilbert and Andrew McBain (School of Pharmacy and Pharmaceutical Sciences, University of Manchester)Chapter 5. Diverse Mechanisms of Biofilm Formation, by Lisa Friedman and Roberto Kolter (Department of Microbiology and Molecular Genetics, Harvard Medical School)Chapter 6. Biofilm Formation and Control in Food Processing Environments, by Amy C. Lee Wong (Department of Food Microbiology and Toxicology, Food Research Institute, University of Wisconsin-Madison)