Expression And Mechanistic Studies On Orotidine 5 Monophosphate Decarboxylase PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Expression And Mechanistic Studies On Orotidine 5 Monophosphate Decarboxylase PDF full book. Access full book title Expression And Mechanistic Studies On Orotidine 5 Monophosphate Decarboxylase.
Author | : Kathryn J. Kitzmiller |
Publisher | : |
Total Pages | : 172 |
Release | : 2004 |
Genre | : Decarboxylases |
ISBN | : |
Download Expression and Mechanistic Studies on Orotidine-5'-monophosphate Decarboxylase Book in PDF, ePub and Kindle
Author | : Jeehiun K. Lee |
Publisher | : Springer |
Total Pages | : 0 |
Release | : 2010-12-01 |
Genre | : Science |
ISBN | : 9783642058196 |
Download Orotidine Monophosphate Decarboxylase Book in PDF, ePub and Kindle
Author | : Ning Wu |
Publisher | : National Library of Canada = Bibliothèque nationale du Canada |
Total Pages | : 360 |
Release | : 2002 |
Genre | : |
ISBN | : 9780612745773 |
Download Structural and Mechanistic Studies of Orotidine 5'-monophosphate Decarboxylase [microform] Book in PDF, ePub and Kindle
Author | : Freeman M. Wong |
Publisher | : |
Total Pages | : 120 |
Release | : 2003 |
Genre | : Decarboxylases |
ISBN | : |
Download Mechanistic Study of Orotidine 5'-monophosphate Decarboxylase Using Uridine 5'-monophosphate Analogues Book in PDF, ePub and Kindle
Author | : Vanessa A. Iiams |
Publisher | : |
Total Pages | : |
Release | : 2011 |
Genre | : |
ISBN | : |
Download Investigations Into the Mechanism of Orotidine 5'-monophosphate Decarboxylase: Sources of Substrate Destabilization and Transition State Stabilization Book in PDF, ePub and Kindle
Orotidine 50́ø-monophosphate decarboxylase (OMPDC) achieves a rarely paralleled rate acceleration, yet the catalytic basis prompting this enhancement have yet to be fully elucidated. To accomplish decarboxylation, OMPDC must overcome the high energy barrier due to the localized anionic charge of the intermediate. Mechanistic studies employing enzyme mutagenesis and product or intermediate analogues were used to investigate possible transition state stabilization by a carbene resonance structure. Viability of the carbene structure depends upon a key hydrogen bond between O4 of the substrate and the amide backbone of a conserved serine or threonine. Substitution of the conserved residue with Pro resulted in a kcat/KM of 1 M-1s-1; deletion of the FUMP O4 resulted in a product analogue that does not undergo H6 exchange or inhibit decarboxylation. Hence, indirect evidence reveals the O4-backbone interaction plays an important role for binding and catalysis. OMPDC likely has honed multiple mechanisms to attain its remarkable catalysis. The successful crystallizations of OMPDC a decade ago sparked hypotheses that structure and sequence conserved residues induced productive strain on the substrate-enzyme complex. Here, we demonstrate a new source of stress: a hydrophobic pocket adjacent to the OMP carboxylate that exhibits kinetic parameters characteristic of substrate destabilization. Substitution of these residues with hydrophilic side-chains, by providing hydrogen-bonding partners, decreased kcat by 10 to 10^40́3fold. The same substitutions display very little change in the rate of product H6 exchange, supporting that this hydrophobic pocket affects the substrate-enzyme complex before the transition state. We also provide evidence that hydrophilic residues can insert water molecules into the pocket with detrimental effects to catalysis.
Author | : |
Publisher | : Academic Press |
Total Pages | : 375 |
Release | : 2001-01-02 |
Genre | : Science |
ISBN | : 0080544533 |
Download Cofactor Biosynthesis: A Mechanistic Perspective Book in PDF, ePub and Kindle
The longest running serial published by Academic Press continues its well-respected run with Volume 61, a special volume in which a guest editor has come on board and has assembled some well-known contributors who are international authorities in the field. Together they tackle some of the latest topics in the field such as riboflavin and folate biosynthesis, biotin and lipoic acid biosynthesis, nicotinamide adenine dinucleotide biosynthesis, biosynthesis of vitamin B6 and structurally related derivatives, pantothenic acid and coenzyme A biosynthesis, mechanistic biosynthesis of protein-derived redox cofactors, ascorbic acid biosynthesis, biosynthesis of menaquinone and ubiquinone - Vitamin B12 biosynthesis, biosynthesis of the methanogenic cofactors, and thiamin biosynthesis.
Author | : Alton Meister |
Publisher | : Wiley-Interscience |
Total Pages | : 0 |
Release | : 1995-12-15 |
Genre | : Science |
ISBN | : 9780471127017 |
Download Advances in Enzymology and Related Areas of Molecular Biology, Volume 71 Book in PDF, ePub and Kindle
Enzymes a revised frequently in modifying proteins for specialized uses. These books cover the latest advances in this field and its applications in the field of molecular biology.
Author | : |
Publisher | : |
Total Pages | : 1202 |
Release | : 1990 |
Genre | : Biology |
ISBN | : |
Download The FASEB Journal Book in PDF, ePub and Kindle
Author | : |
Publisher | : |
Total Pages | : 1674 |
Release | : 1995 |
Genre | : Medical genetics |
ISBN | : |
Download The Metabolic and Molecular Bases of Inherited Disease Book in PDF, ePub and Kindle
Presents clinical, biochemical, and genetic information concerning those metabolic anomalies grouped under inborn errors of metabolism.
Author | : |
Publisher | : |
Total Pages | : 122 |
Release | : 2003 |
Genre | : Coenzymes |
ISBN | : |
Download Coenzymes, Cofactors and Catalysis [program and Abstracts] Book in PDF, ePub and Kindle