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| Author | : Tanja Angela Schüttrigkeit |
| Publisher | : |
| Total Pages | : 156 |
| Release | : 2004 |
| Genre | : |
| ISBN | : |
| Author | : |
| Publisher | : |
| Total Pages | : |
| Release | : 2006 |
| Genre | : |
| ISBN | : |
Deactivation channels of excited states in green and red fluorescing proteins (GFP, RFP) and mutants as well as flavoproteins have been investigated by steady-state and femtosecond/picosecond time-resolved fluorescence/absorption spectroscopy. Besides fast internal conversion, energy transfer between chromophores in different maturation states in oligomers of DsRed as well as from the antenna molecule to the flavin cofactor in (6-4) photolyase and cryptochrom2 has been established as photophysical primary process. In cryptochrom1, electron transfer from nearby tyrosyl and tryptophane residues to FAD is the initial step in signal transduction, as is intersystem crossing in the LOV2 domain in phototropin.
| Author | : Paul Abbyad |
| Publisher | : |
| Total Pages | : 344 |
| Release | : 2007 |
| Genre | : |
| ISBN | : |
| Author | : Gregor Jung |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 275 |
| Release | : 2011-10-25 |
| Genre | : Science |
| ISBN | : 3642233724 |
Fluorescent proteins are intimately connected to research in the life sciences. Tagging of gene products with fluorescent proteins has revolutionized all areas of biosciences, ranging from fundamental biochemistry to clinical oncology, to environmental research. The discovery of the Green Fluorescent Protein, its first, seminal application and the ingenious development of a broad palette of fluorescence proteins of other colours, was consequently recognised with the Nobel Prize for Chemistry in 2008. Fluorescent Proteins I is devoted to the basic photophysical and photochemical aspects of fluorescent protein technology. Experienced experts highlight colour tuning, the exploration of switching phenomena and respective methods for their investigation. The book provides a thorough understanding of primary molecular processes allowing the design of fluorescent proteins for specific applications.
| Author | : Xinghua Shi |
| Publisher | : |
| Total Pages | : 456 |
| Release | : 2007 |
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| Author | : American Society for Cell Biology |
| Publisher | : Gulf Professional Publishing |
| Total Pages | : 430 |
| Release | : 1999 |
| Genre | : Medical |
| ISBN | : 9780126760750 |
This volume is an authoritative and comprehensive treatment of the approaches and techniques used for Green Fluorescent Proteins (GFP). The primary focus of this work is on research using biological systems. The volume covers all aspects of GFP, from its expression in different organisms to specific microscopic and data analysis methods. Key Features * Only volume on Green Fluorescent Protein research * Covers all aspects of GFP * Provides specific microscopic and data analysis methods * Discusses the design and construction of GFP fusion proteins * Covers GFP expression in animals, insects, plants, and microbes * Details procedures for time lapse imaging of living cells * Explains how to implement single molecule fluorescence detection with GFP * Discusses dual label GFP strategies for multicolor fluorescence * Presents fluorescence resonance energy transfer methods with GFPs * Details quantitative fluorescence imaging techniques * Extensively illustrated with color photographs
| Author | : Richard N. Day |
| Publisher | : CRC Press |
| Total Pages | : 340 |
| Release | : 2014-04-28 |
| Genre | : Science |
| ISBN | : 143987509X |
Advances in fluorescent proteins, live-cell imaging, and superresolution instrumentation have ushered in a new era of investigations in cell biology, medicine, and physiology. From the identification of the green fluorescent protein in the jellyfish Aequorea victoria to the engineering of novel fluorescent proteins, The Fluorescent Protein Revoluti
| Author | : Gregor Jung |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 287 |
| Release | : 2012-01-05 |
| Genre | : Science |
| ISBN | : 3642233767 |
Fluorescent proteins are intimately connected to research in the life sciences. Tagging of gene products with fluorescent proteins has revolutionized all areas of biosciences, ranging from fundamental biochemistry to clinical oncology, to environmental research. The discovery of the Green Fluorescent Protein, its first, seminal application and the ingenious development of a broad palette of fluorescence proteins of other colours, was consequently recognised with the Nobel Prize for Chemistry in 2008. Fluorescent Proteins II highlights the physicochemical and biophysical aspects of fluorescent protein technology beyond imaging. It is tailored to meet the needs of physicists, chemists and biologists who are interested in the fundamental properties of fluorescent proteins, while also focussing on specific applications. The implementations described are cutting-edge studies and exemplify how the physical and chemical properties of fluorescent proteins can stimulate novel findings in life sciences.
| Author | : Martin Chalfie |
| Publisher | : John Wiley & Sons |
| Total Pages | : 486 |
| Release | : 2005-11-18 |
| Genre | : Science |
| ISBN | : 0471739480 |
Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications. This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.
| Author | : Brett Andrew King |
| Publisher | : |
| Total Pages | : 514 |
| Release | : 2000 |
| Genre | : |
| ISBN | : |
