Enzymological Studies Of One Carbon Reactions In The Pathway Of Acetate Utilization By Methanogenic Bacteria PDF Download
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Total Pages | : 5 |
Release | : 1991 |
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Download Enzymological Studies of One-carbon Reactions in the Pathway of Acetate Utilization by Methanogenic Bacteria Book in PDF, ePub and Kindle
Several enzymes in the pathway of acetate conversion to methane and carbon dioxide have been purified from Methanosarcina thermophila. The mechanisms of these enzymes are under investigation utilizing biochemical, biophysical and molecular genetic approaches. Acetate kinase and phosphotransacetylase catalyzes the activation of acetate to acetyl-CoA. The primary structure of these enzymes will be determined through cloning and sequencing of the genes. Two protein components of the CO dehydrogenase complex are under investigations. The metal centers of each component have been characterized using EPR. Cloning and sequencing of the genes for the two subunits of each component is in progress. Results indicate that the Ni/Fe-S component cleaves the C-C and C-S bonds of acetyl-CoA followed by oxidation of the carbonyl group to carbon dioxide and transfer of the methyl group to the Co/Fe-S component. The enzymes and cofactors involved in transfer of the methyl group from the Co/Fe-S component to coenzyme M will be purified and characterized. Ferredoxin is an electron acceptor for the Ni/Fe-S component and also serves to reductively reactivate methylreductase which catalyzes the demethylation of methyl coenzyme M to methane. This ferredoxin is being characterized utilizing EPR and RR spectroscopic methods to determine the properties of the Fe-S centers. Genes encoding this and other ferredoxins have been cloned and sequenced to determine the primary structures. Carbonic anhydrase is being purified and characterized to determine the function of this enzyme in the pathway.
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Total Pages | : 754 |
Release | : 1993 |
Genre | : Power resources |
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Download Energy Research Abstracts Book in PDF, ePub and Kindle
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Total Pages | : 908 |
Release | : 1988 |
Genre | : Power resources |
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Includes all works deriving from DOE, other related government-sponsored information and foreign nonnuclear information.
Author | : United States. Congress. House. Committee on Appropriations. Subcommittee on Energy and Water Development |
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Total Pages | : 1300 |
Release | : 1997 |
Genre | : Energy development |
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Download Energy and Water Development Appropriations for 1998: Secretary of Energy Book in PDF, ePub and Kindle
Author | : United States. Congress. House. Committee on Appropriations. Subcommittee on Energy and Water Development |
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Total Pages | : 1918 |
Release | : 1997 |
Genre | : Energy development |
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Download Energy and Water Development Appropriations for 1998 Book in PDF, ePub and Kindle
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Total Pages | : 1180 |
Release | : 199? |
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Download Government reports annual index Book in PDF, ePub and Kindle
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Total Pages | : 1132 |
Release | : 1988 |
Genre | : Science |
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Download Government Reports Announcements & Index Book in PDF, ePub and Kindle
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Total Pages | : 1600 |
Release | : 1988 |
Genre | : Government reports announcements & index |
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Download Government Reports Annual Index: Keyword A-L Book in PDF, ePub and Kindle
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Total Pages | : 852 |
Release | : 1992-10 |
Genre | : Science |
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Author | : Edward F. DeLong |
Publisher | : Springer |
Total Pages | : 567 |
Release | : 2014-10-13 |
Genre | : Science |
ISBN | : 9783642301193 |
Download The Prokaryotes Book in PDF, ePub and Kindle
The Prokaryotes is a comprehensive, multi-authored, peer reviewed reference work on Bacteria and Achaea. This fourth edition of The Prokaryotes is organized to cover all taxonomic diversity, using the family level to delineate chapters. Different from other resources, this new Springer product includes not only taxonomy, but also prokaryotic biology and technology of taxa in a broad context. Technological aspects highlight the usefulness of prokaryotes in processes and products, including biocontrol agents and as genetics tools. The content of the expanded fourth edition is divided into two parts: Part 1 contains review chapters dealing with the most important general concepts in molecular, applied and general prokaryote biology; Part 2 describes the known properties of specific taxonomic groups. Two completely new sections have been added to Part 1: bacterial communities and human bacteriology. The bacterial communities section reflects the growing realization that studies on pure cultures of bacteria have led to an incomplete picture of the microbial world for two fundamental reasons: the vast majority of bacteria in soil, water and associated with biological tissues are currently not culturable, and that an understanding of microbial ecology requires knowledge on how different bacterial species interact with each other in their natural environment. The new section on human microbiology deals with bacteria associated with healthy humans and bacterial pathogenesis. Each of the major human diseases caused by bacteria is reviewed, from identifying the pathogens by classical clinical and non-culturing techniques to the biochemical mechanisms of the disease process. The 4th edition of The Prokaryotes is the most complete resource on the biology of prokaryotes. The following volumes are published consecutively within the 4th Edition: Prokaryotic Biology and Symbiotic Associations Prokaryotic Communities and Ecophysiology Prokaryotic Physiology and Biochemistry Applied Bacteriology and Biotechnology Human Microbiology Actinobacteria Firmicutes Alphaproteobacteria and Betaproteobacteria Gammaproteobacteria Deltaproteobacteria and Epsilonproteobacteria Other Major Lineages of Bacteria and the Archaea