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Enzymes and DNA Recombinant Technology

Enzymes and DNA Recombinant Technology
Author: Shafat Ahmad Latoo
Publisher:
Total Pages: 0
Release: 2024
Genre: Enzymes
ISBN:

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"Enzymes represent one of the most fascinating and meaningful entities in the biological world. In the fields of genetic engineering and recombinant DNA technology, enzymes serve as vital tools. Enzymes acting as biological catalysts have been known to all of us since our primary education. Nonetheless, learning about enzymes at the graduate level can be difficult by a lack of comprehension of the principles and a misunderstanding of the terminology employed in enzymology. This book has been written to augment the understanding of enzymology among graduate and postgraduate students. The author of this book has extensive experience teaching enzymology and has proven and admired competence in the field. Balanced text, well-illustrated concepts, and an organized stepwise ascent of the topics are key features of this book. Besides fundamentals, this book also covers some of the emerging areas of enzymology, such as enzyme immobilization, reversible covalent modification, and proteolytic activation. This book not only provides information for enzymologists but also does so in a manner that will also aid non-enzymologists in making proper use of these biocatalysts in their research. This book consists of a detailed discussion of key concepts of enzymology, enzyme kinetics, modes of enzyme regulation, isozymes, enzyme immobilization, DNA cloning, and genome editing. Difficult concepts have been simplified with analogies and examples. Furthermore, this book's material aligns with the majority of university curriculum and exams, both domestically and internationally. Key Features: - Written in a simple and understandable language. - Covers fundamental concepts. - Presents information in an easily accessible format to serve as a quick reference source. - A concise write-up for addressing dullness while going through it. - Provides essential basics as well as up-to-date information on enzymes and their use in recombinant DNA technology, and genome editing"--


Enzymology Primer for Recombinant DNA Technology

Enzymology Primer for Recombinant DNA Technology
Author: Hyone-Myong Eun
Publisher: Elsevier
Total Pages: 727
Release: 1996-06-03
Genre: Science
ISBN: 008053113X

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Enzymes are indispensable tools in recombinant DNA technology and genetic engineering. This book not only provides information for enzymologists, but does so in a manner that will also aid nonenymologists in making proper use of these biocatalysts in their research. The Enzymology Primer for Recombinant DNA Technology includes information not usually found in the brief descriptions given in most books on recombinant DNA methodology and gene cloning. Provides essential basics as well as up-to-date information on enzymes most commonly used in recombinant DNA technology Presents information in an easily accessible format to serve as a quick reference source Leads to a better understanding of the role of biocatalysts in recombinant DNA techniques


Recombinant DNA Technology

Recombinant DNA Technology
Author: Siddra Ijaz
Publisher: Cambridge Scholars Publishing
Total Pages: 156
Release: 2019-08-13
Genre: Science
ISBN: 1527538443

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Genetic engineering is a rapidly growing field in the area of biological sciences. The driving forces behind this are the challenges encountered by health sectors, agriculture, the environment, and industry. As such, accurate and comprehensive knowledge about the philosophy, principles and application of genetic engineering is indispensable for students and researchers to harness maximum opportunities from this field of science. This volume gathers together comprehensive information regarding genetic engineering from recent studies, and presents it in a coherent manner. As such, it will be of interest to undergraduate and postgraduate students and researchers working in the biological sciences.


Recombinant DNA Technology

Recombinant DNA Technology
Author: Aleš Prokop
Publisher: Allied Publishers
Total Pages: 280
Release:
Genre:
ISBN: 9788170239956

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Recombinant DNA Technology

Recombinant DNA Technology
Author: Keya Chaudhuri
Publisher: The Energy and Resources Institute (TERI)
Total Pages: 300
Release: 2013-01-01
Genre: Science
ISBN: 8179933202

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Recombinant DNA Technology is focussed on the current state of knowledge on the recombinant DNA technology and its applications. The book will provide comprehensive knowledge on the principles and concepts of recombinant DNA technology or genetic engineering, protein expression of cloned genes, PCR amplification of DNA, RFLP, AFLP and DNA fingerprinting and finally the most recent siRNA technology. It can be used by post-graduate students studying and teachers teaching in the area of Molecular Biology, Biotechnology, Genetics, Microbiology, Life Science, Pharmacy, Agriculture and Basic Medical Sciences.


Calculations for Molecular Biology and Biotechnology

Calculations for Molecular Biology and Biotechnology
Author: Frank H. Stephenson
Publisher: Academic Press
Total Pages: 520
Release: 2010-07-30
Genre: Science
ISBN: 012375691X

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Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition, provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing. Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation Recent applications of the procedures and computations in clinical, academic, industrial and basic research laboratories are cited throughout the text New to this Edition: Updated and increased coverage of real time PCR and the mathematics used to measure gene expression More sample problems in every chapter for readers to practice concepts


Recombinant DNA Methodology

Recombinant DNA Methodology
Author:
Publisher: Academic Press
Total Pages: 793
Release: 2014-05-19
Genre: Science
ISBN: 1483295974

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Recombinant DNA methods are powerful, revolutionary techniques that allow the isolation of single genes in large amounts from a pool of thousands or millions of genes and the modification of these isolated genes or their regulatory regions for reintroduction into cells for expression at the RNA or protein levels. These attributes lead to the solution of complex biological problems and the production of new and better products in the areas of medicine, agriculture, and industry. Recombinant DNA Methodology, a volume in the Selected Methods in Enzymology series produced in benchtop format, contains a selection of key articles from Volumes 68, 100, 101, 153, 154, and 155 of Methods in Enzymology. The essential and widely used procedures provided at an affordable price will be an invaluable aid to the graduate student and the researcher. Enzymes in DNA research DNA isolation, hybridization, and cloning DNA sequence analysis cDNA cloning Gene products Identification of cloned genes and mapping of genes Monitoring cloned gene expression Cloning and transferring of genes into yeast cells Cloning and transferring of genes into plant cells Cloning and transferring of genes into animal cells Site-directed mutagenesis Protein engineering Expression vectors


Introduction to Basics of Pharmacology and Toxicology

Introduction to Basics of Pharmacology and Toxicology
Author: Gerard Marshall Raj
Publisher: Springer Nature
Total Pages: 410
Release: 2019-11-16
Genre: Medical
ISBN: 9813297794

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This book illustrates, in a comprehensive manner, the most crucial principles involved in pharmacology and allied sciences. The title begins by discussing the historical aspects of drug discovery, with up to date knowledge on Nobel Laureates in pharmacology and their significant discoveries. It then examines the general pharmacological principles - pharmacokinetics and pharmacodynamics, with in-depth information on drug transporters and interactions. In the remaining chapters, the book covers a definitive collection of topics containing essential information on the basic principles of pharmacology and how they are employed for the treatment of diseases. Readers will learn about special topics in pharmacology that are hard to find elsewhere, including issues related to environmental toxicology and the latest information on drug poisoning and treatment, analytical toxicology, toxicovigilance, and the use of molecular biology techniques in pharmacology. The book offers a valuable resource for researchers in the fields of pharmacology and toxicology, as well as students pursuing a degree in or with an interest in pharmacology.


DNA Technology in Forensic Science

DNA Technology in Forensic Science
Author: National Research Council
Publisher: National Academies Press
Total Pages: 199
Release: 1992-02-01
Genre: Science
ISBN: 0309045878

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Matching DNA samples from crime scenes and suspects is rapidly becoming a key source of evidence for use in our justice system. DNA Technology in Forensic Science offers recommendations for resolving crucial questions that are emerging as DNA typing becomes more widespread. The volume addresses key issues: Quality and reliability in DNA typing, including the introduction of new technologies, problems of standardization, and approaches to certification. DNA typing in the courtroom, including issues of population genetics, levels of understanding among judges and juries, and admissibility. Societal issues, such as privacy of DNA data, storage of samples and data, and the rights of defendants to quality testing technology. Combining this original volume with the new update-The Evaluation of Forensic DNA Evidence-provides the complete, up-to-date picture of this highly important and visible topic. This volume offers important guidance to anyone working with this emerging law enforcement tool: policymakers, specialists in criminal law, forensic scientists, geneticists, researchers, faculty, and students.


Recombinant DNA and Biotechnology

Recombinant DNA and Biotechnology
Author: Helen Kreuzer
Publisher:
Total Pages: 572
Release: 1996
Genre: Medical
ISBN:

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Laying the foundation; An averview of biotechnology; Genes, genetics, and geneticists; An overview of molecular of molecular biology: recombinant DNA technology; Classroom activities; DNA structure and function; Constructing a paper helix; DNA replication; From genes to proteins;Sizes of the Escherichia coli and human genomes; Extraction of bacterial DNA; Manipulation and analysis of DNA; DNA scissors: introduction to restriction enzymes; DNA goes to the races; Gel electrophoresis of precut lambda DNA; Recombinant paper plasmids; Restriction analysis challenge worksheets; Detection of specific DNA sequences; DNA sequencing; The polymerase chain reaction: paper PCR; Transfer of genetic information; Trasformation of Escherichia coli; Conjugative transfer of antibiotic resistance in Escherichia coli; Transduction of an antibiotic resistance gene; Agrobacterium tumefaciens: nature's plant genetic engineer; Analysing genetic variation; Generating genetic variation: the meiosis game; Analysing genetic variation: DNA typing; A mix-up at the hospital; A paternity case; The case of the bloody knife; The molecularbasis of genetic diseases; Societal issues; Science, Technology, and society; Weighing technology's risks and benefits; Debating the risks of biotechnology; A decision-making model for bioethical issues; BBioethics case study: gene therapy; Bioethics case study: genetic screening; Careers in biotechnology; Appendixes; Laboratory biosafety; Basis microbiological methods; Aseptic technique; Sterilization of equipment and media; Recipes; Biotechnology laboratory equipment; Using the equipment; Recommended reading; Teaching resources; National science education standards and the content of this book; Templates; Overhead masters.