Effects Of Solution Parameters On The Adsorption Of Proteins At Interfaces PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Effects Of Solution Parameters On The Adsorption Of Proteins At Interfaces PDF full book. Access full book title Effects Of Solution Parameters On The Adsorption Of Proteins At Interfaces.
Author | : Christina Regina Vessely |
Publisher | : |
Total Pages | : 346 |
Release | : 2006 |
Genre | : |
ISBN | : |
Download Effects of Solution Parameters on the Adsorption of Proteins at Interfaces Book in PDF, ePub and Kindle
Author | : |
Publisher | : |
Total Pages | : 561 |
Release | : 1995 |
Genre | : |
ISBN | : |
Download Proteins at Interfaces II Book in PDF, ePub and Kindle
Author | : Eduardo Guzmán |
Publisher | : MDPI |
Total Pages | : 232 |
Release | : 2021-03-02 |
Genre | : Science |
ISBN | : 3039436333 |
Download Fluid Interfaces Book in PDF, ePub and Kindle
Fluid interfaces are promising candidates for confining different types of materials, e.g., polymers, surfactants, colloids, and even small molecules, to be used in designing new functional materials with reduced dimensionality. The development of such materials requires a deepening of the physicochemical bases underlying the formation of layers at fluid interfaces as well as on the characterization of their structures and properties. This is of particular importance because the constraints associated with the assembly of materials at the interface lead to the emergence of equilibrium and features of dynamics in the interfacial systems, which are far removed from those conventionally found in traditional materials. This Special Issue is devoted to studies on the fundamental and applied aspects of fluid interfaces, and attempts to provide a comprehensive perspective on the current status of the research field.
Author | : Zhenzhu Xu |
Publisher | : |
Total Pages | : 176 |
Release | : 2018 |
Genre | : |
ISBN | : |
Download Protein Adsorption on Metal Oxide Nanoparticle Surfaces Book in PDF, ePub and Kindle
With the development of nanoscience and nanotechnology, the biocompatibility of nanomaterials is becoming increasingly important. As one of the most prevalent nanomaterials, metal oxide nanoparticles in particular are receiving growing attention due to their potential negative impacts to the environmental and human health upon exposure. Nanoparticles, once introduced in biological fluids, can adsorb proteins forming protein corona, which can cause protein conformational change and function loss. Although many studies have been focusing on the above effects, details of protein interaction with nanoparticles and consequential structural change on nanoparticle surfaces still remain unclear as these processes can be affected by various factors. Therefore, a systematic study on the impacts of influential factors on the behavior of proteins at the nano-bio interface is strongly desired. The research in the present dissertation pursues a greater understanding of the effects of various parameters on the protein-nanoparticle surface interactions and protein structural change upon adsorption. Specifically, nanoparticle- and environmental-related factors including nanoparticle surface chemistry, pH, co-adsorption of phosphate, and temperature were explored. Spectroscopic and thermogravimetric analysis of bovine serum albumin (BSA) adsorption on TiO2 and SiO2 nanoparticles as a function of pH highlighted the importance of both pH and nanoparticle surface chemistry on protein behavior at nano-bio interfaces. The study shows that protein interaction is strongest on TiO2 surface. Especially, at acidic pH, BSA is completely denatured on TiO2 and protein surface coverage was a factor of three to ten times higher than on SiO2. Detailed secondary structural analysis of BSA adsorption with and without the presence of phosphate indicates that the co-adsorption of phosphate could prevent surface-induced denaturation at very acidic conditions. Furthermore, the effects of surface adsorption on temperature-dependent conformational change of BSA and fibrinogen (Fib) on TiO2 nanoparticles was investigated. Adsorbed BSA exhibits much less change in its secondary structure with increasing temperature than its solution phase, whereas Fib in dissolved and adsorbed states display very similar trend in their structural change as a function of temperature. Moreover, to complement our understanding of protein adsorption on nanoparticles, hydrogen/deuterium exchange mass spectrometry was carried out as a novel method to investigate the role of specific amino acid residues in protein-surface interaction. Thus, the research in this dissertation provide important insights into understanding the behavior of proteins at the nano-bio interface, specifically the effects of various influences on protein-surface interaction and protein structure.
Author | : C. H. Bamford |
Publisher | : VSP |
Total Pages | : 218 |
Release | : 1992-12-01 |
Genre | : Science |
ISBN | : 9789067641395 |
Download The Vroman Effect Book in PDF, ePub and Kindle
The development of specific antibodies as probes and detectors for adsorbed proteins by Dr. Leo Vroman and co-workers in the 1960s and 1970s confirmed his earlier observations and suspicions that blood protein adsorption involved a hierarchical series of collision, adsorption, and exchange processes. These observations and concepts were confirmed by other scientists and came to be known as 'the Vroman effect'. The core concept of the Vroman effect admits many approaches and over-reaches complex and not fully resolved questions of enzymology, transport phenomena, the statistical mechanics of protein conformation, longrange forces in liquids, and surface physics. This volume contains the presentations from the symposium which was held in honour of the 75th birhday of Dr. Leo Vroman, in Gouda, The Netherlands, and deals with various aspects of the Vroman effect.
Author | : Juan M. Ruso |
Publisher | : John Wiley & Sons |
Total Pages | : 823 |
Release | : 2013-01-31 |
Genre | : Science |
ISBN | : 1118523172 |
Download Proteins in Solution and at Interfaces Book in PDF, ePub and Kindle
Explores new applications emerging from our latest understanding of proteins in solution and at interfaces Proteins in solution and at interfaces increasingly serve as the starting point for exciting new applications, from biomimetic materials to nanoparticle patterning. This book surveys the state of the science in the field, offering investigators a current understanding of the characteristics of proteins in solution and at interfaces as well as the techniques used to study these characteristics. Moreover, the authors explore many of the new and emerging applications that have resulted from the most recent studies. Topics include protein and protein aggregate structure; computational and experimental techniques to study protein structure, aggregation, and adsorption; proteins in non-standard conditions; and applications in biotechnology. Proteins in Solution and at Interfaces is divided into two parts: Part One introduces concepts as well as theoretical and experimental techniques that are used to study protein systems, including X-ray crystallography, nuclear magnetic resonance, small angle scattering, and spectroscopic methods Part Two examines current and emerging applications, including nanomaterials, natural fibrous proteins, and biomolecular thermodynamics The book's twenty-three chapters have been contributed by leading experts in the field. These contributions are based on a thorough review of the latest peer-reviewed findings as well as the authors' own research experience. Chapters begin with a discussion of core concepts and then gradually build in complexity, concluding with a forecast of future developments. Readers will not only gain a current understanding of proteins in solution and at interfaces, but also will discover how theoretical and technical developments in the field can be translated into new applications in material design, genetic engineering, personalized medicine, drug delivery, biosensors, and biotechnology.
Author | : Horst W. Doelle |
Publisher | : EOLSS Publications |
Total Pages | : 372 |
Release | : 2009-11-16 |
Genre | : Biochemical engineering |
ISBN | : 1848262582 |
Download BIOTECHNOLOGY - Volume IV Book in PDF, ePub and Kindle
This Encyclopedia of Biotechnology is a component of the global Encyclopedia of Life Support Systems (EOLSS), which is an integrated compendium of twenty one Encyclopedias. Biotechnology draws on the pure biological sciences (genetics, animal cell culture, molecular biology, microbiology, biochemistry, embryology, cell biology) and in many instances is also dependent on knowledge and methods from outside the sphere of biology (chemical engineering, bioprocess engineering, information technology, biorobotics). This 15-volume set contains several chapters, each of size 5000-30000 words, with perspectives, applications and extensive illustrations. It carries state-of-the-art knowledge in the field and is aimed, by virtue of the several applications, at the following five major target audiences: University and College Students, Educators, Professional Practitioners, Research Personnel and Policy Analysts, Managers, and Decision Makers and NGOs.
Author | : Philippe Déjardin |
Publisher | : Springer Science & Business Media |
Total Pages | : 341 |
Release | : 2006-09-28 |
Genre | : Science |
ISBN | : 3540326588 |
Download Proteins at Solid-Liquid Interfaces Book in PDF, ePub and Kindle
This book opens with a description of fundamental aspects of protein adsorption to surfaces, a phenomenon that plays a key role in biotechnological applications, especially at solid-liquid interfaces. Presented here are methods for studying adsorption kinetics and conformational changes such as optical waveguide lightmode spectroscopy (OWLS). Also described are sensitive bench techniques for measuring the orientation and structure of proteins at solid-liquid interfaces, including total internal reflection ellipsometry (TIRE), dual polarisation interferometry (DPI) and time of flight - secondary ion mass spectrometry (TOF–SIMS). A model study of fibronectin at polymer surfaces is included, as are studies using microporous membranes and textiles with immobilized enzymes for large-scale applications. Biocompatibility, anti-fouling properties and surface modification to modulate the adsorption and activity of biomolecules are among the other topics addressed in this invaluable book.
Author | : D. Möbius |
Publisher | : Elsevier |
Total Pages | : 509 |
Release | : 1998-04-24 |
Genre | : Science |
ISBN | : 0080540007 |
Download Proteins at Liquid Interfaces Book in PDF, ePub and Kindle
The interfacial behaviour of surfactants and proteins, and their mixtures, is of importance in a wide range of areas such as food technology, detergency, cosmetics, coating processes, biomedicine, pharmacy and biotechnology. Methods such as surface and interfacial tension measurements and interfacial dilation and shear rheology characterise the relationships between these interfacial properties and the complex behaviour of foams and emulsions is established. Recently-developed experimental techniques, such as FRAP which enable the measurement of molecular mobility in adsorption layers, are covered in this volume. The development of theories to describe the thermodynamic surface state or the exchange of matter for proteins and protein/surfactant mixtures is also described. Features of this book: • Reflects the state-of-the-art research and application of protein interfacial layers rather than a snapshot of only some recent developments. • Emphasis is placed on experimental details as well as recent theoretical developments. • New experimental techniques applied to protein interfacial layers are described, such as FRAP or ADSA, or rheological methods to determine the mechanical behaviour of protein-modified interfaces. • A large number of practical applications, ranging from emulsions relevant in food technology for medical problems such as lung surfactants, to the characterisation of foams intrinsic to beer and champagne production. The book will be of interest to research and university institutes dedicated to interfacial studies in chemistry, biology, pharmacy, medicine and food engineering. Industrial departments for research and technology in food industry, pharmacy, medicine and brewery research will also find this volume of value.
Author | : Kyung Bin Song |
Publisher | : |
Total Pages | : 596 |
Release | : 1989 |
Genre | : |
ISBN | : |
Download Protein Adsorption at Fluid Interfaces Book in PDF, ePub and Kindle