Dynamics Of Nuclear Architecture Investigated By Live Cell Microscopy And Quantitative 4 D Reconstruction PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Dynamics Of Nuclear Architecture Investigated By Live Cell Microscopy And Quantitative 4 D Reconstruction PDF full book. Access full book title Dynamics Of Nuclear Architecture Investigated By Live Cell Microscopy And Quantitative 4 D Reconstruction.
Author | : Daniel Gerlich |
Publisher | : |
Total Pages | : 68 |
Release | : 2002 |
Genre | : |
ISBN | : |
Download Dynamics of Nuclear Architecture Investigated by Live Cell Microscopy and Quantitative 4-D Reconstruction Book in PDF, ePub and Kindle
Author | : Christophe Lavelle |
Publisher | : Academic Press |
Total Pages | : 620 |
Release | : 2017-10-27 |
Genre | : Science |
ISBN | : 012803503X |
Download Nuclear Architecture and Dynamics Book in PDF, ePub and Kindle
Nuclear Architecture and Dynamics provides a definitive resource for (bio)physicists and molecular and cellular biologists whose research involves an understanding of the organization of the genome and the mechanisms of its proper reading, maintenance, and replication by the cell. This book brings together the biochemical and physical characteristics of genome organization, providing a relevant framework in which to interpret the control of gene expression and cell differentiation. It includes work from a group of international experts, including biologists, physicists, mathematicians, and bioinformaticians who have come together for a comprehensive presentation of the current developments in the nuclear dynamics and architecture field. The book provides the uninitiated with an entry point to a highly dynamic, but complex issue, and the expert with an opportunity to have a fresh look at the viewpoints advocated by researchers from different disciplines. Highlights the link between the (bio)chemistry and the (bio)physics of chromatin Deciphers the complex interplay between numerous biochemical factors at task in the nucleus and the physical state of chromatin Provides a collective view of the field by a large, diverse group of authors with both physics and biology backgrounds
Author | : |
Publisher | : |
Total Pages | : |
Release | : 2003 |
Genre | : |
ISBN | : |
Download Modelling Nuclear Body Dynamics in Living Cells by 4-D Microscopy, Image Analysis and Simulation Book in PDF, ePub and Kindle
The work presented here demonstrates rules of and validates models for nuclear body (NB) dynamics. Simulation tools developed in the course of this work can be used in future work to generate hypotheses about related aspects of nuclear architecture. Initially I examined the mobility of vimentin nuclear bodies bodies (VNB) in interphase by single particle tracking and analysis of fluorescence images from 4-D confocal laser scanning microscopy (CLSM). These synthetic nuclear bodies are observed in cells transfected with labelled nuclear-targeted Xenopus laevis vimentin. Analysis shows that VNBs undergo anomalous diffusion in the nuclei, independent of metabolic energy. Individual bodies display either one of the three modes of diffusion -- directed, restricted or simple. The consistency of modes and magnitudes of diffusion constants between VNBs and bona fide nuclear bodies points to a generic mechanism that mediates and regulates the mobility of nuclear bodies. Since the results of diffusion analysis of VNBs did not agree with a simple diffusion model, I tested the alternative interchromosomal domain (ICD) compartment model. The ICD model predicts that in interphase cell nuclei, individual decompacted chromosomes do not intermingle, but are separated by a significant interchromatin space forming a network of channels. These networks could affect the mobility of nuclear bodies. Monte Carlo simulations that predict the effects of channels and other obstructions on NB diffusion were tested, but they could not explain deviation from ideal behaviour. Fitting an empirical model of `critical diffusion' produced similar results. Therefore the ICD model as a purely obstructing network of channels needs modification, to possibly include binding. To examine the role of chromatin density in intra-nuclear diffusion, I employed multidimensional fluorescence recovery after photobleaching (FRAP) in living cells. The influence of chromatin density on diffusive mobility of the nuclear.
Author | : Hilmar Strickfaden |
Publisher | : |
Total Pages | : 0 |
Release | : 2010 |
Genre | : |
ISBN | : |
Download Nuclear architecture explored by live-cell fluorescence microscopy using laser and ion microbeam irradiation Book in PDF, ePub and Kindle
Author | : Chaitanya A. Athale |
Publisher | : |
Total Pages | : 0 |
Release | : 2003 |
Genre | : |
ISBN | : |
Download Modelling Nuclear Body Dynamics in Living Cells by 4-D Microscopy, Image Analysis and Simulation Book in PDF, ePub and Kindle
Author | : Célia Baroux |
Publisher | : |
Total Pages | : 659 |
Release | : 2018 |
Genre | : Botany |
ISBN | : 9781493973187 |
Download Plant Chromatin Dynamics Book in PDF, ePub and Kindle
Author | : Yan Shi |
Publisher | : Frontiers Media SA |
Total Pages | : 116 |
Release | : 2021-11-15 |
Genre | : Medical |
ISBN | : 2889716341 |
Download The Role of Biomembranes and Biophysics in Immune Cell Signaling Book in PDF, ePub and Kindle
Author | : |
Publisher | : |
Total Pages | : 700 |
Release | : 2002 |
Genre | : Cells |
ISBN | : |
Download Molecular Biology of the Cell Book in PDF, ePub and Kindle
MBC online publishes papers that describe and interpret results of original research conserning the molecular aspects of cell structure and function.
Author | : Xiaobing Chen |
Publisher | : Frontiers Media SA |
Total Pages | : 195 |
Release | : 2022-09-27 |
Genre | : Science |
ISBN | : 2832500846 |
Download Quantifying and Controlling the Nano-Architecture of Neuronal Synapses Book in PDF, ePub and Kindle
Author | : Mark C. Leake |
Publisher | : Springer Nature |
Total Pages | : 332 |
Release | : 2022-05-30 |
Genre | : Science |
ISBN | : 1071622218 |
Download Chromosome Architecture Book in PDF, ePub and Kindle
This detailed new edition collects cutting-edge laboratory protocols, techniques, and applications in use by some of the leading international experts in the broad field of chromosome architecture. The book emphasizes the increasing physiological relevance of chromosome architecture investigation, manifest both through application of more complex bottom-up assays in vitro as well as through maintaining the native physiological context through the investigation of living, functional cells. In addition, the chapters reflect the dramatic improvement in the length scale of precision by utilizing single-molecule approaches, both for imaging the DNA content of chromosome and proteins that bind to DNA as well as using methods that can controllably manipulate single DNA molecules, and the use of advanced computational methods and mathematical analysis is also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Chromosome Architecture: Methods and Protocols, Second Edition is an ideal guide for researchers working in this dynamic area of study.