Dna Cloning A Hands On Approach PDF Download
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Author | : Seok-Yong Choi |
Publisher | : Springer |
Total Pages | : 131 |
Release | : 2019-04-17 |
Genre | : Medical |
ISBN | : 9402416625 |
Download DNA Cloning: A Hands-on Approach Book in PDF, ePub and Kindle
This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.
Author | : Seok-Yong Choi |
Publisher | : |
Total Pages | : 139 |
Release | : 2019 |
Genre | : Clone cells |
ISBN | : 9789402416619 |
Download DNA Cloning Book in PDF, ePub and Kindle
This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.
Author | : David M. Glover |
Publisher | : |
Total Pages | : |
Release | : 1985 |
Genre | : Molecular cloning |
ISBN | : |
Download DNA Cloning Book in PDF, ePub and Kindle
Author | : David M. Glover |
Publisher | : |
Total Pages | : 269 |
Release | : 1995 |
Genre | : |
ISBN | : |
Download DNA Cloning Book in PDF, ePub and Kindle
Author | : David M. Glover |
Publisher | : |
Total Pages | : 269 |
Release | : 1995 |
Genre | : |
ISBN | : |
Download DNA Cloning Book in PDF, ePub and Kindle
Author | : David M. Glover |
Publisher | : |
Total Pages | : |
Release | : 1986 |
Genre | : |
ISBN | : |
Download DNA cloning Book in PDF, ePub and Kindle
Author | : D. M. Glover |
Publisher | : |
Total Pages | : 245 |
Release | : 1985 |
Genre | : |
ISBN | : 9780947946197 |
Download DNA Cloning Book in PDF, ePub and Kindle
Author | : Joseph Sambrook |
Publisher | : |
Total Pages | : 0 |
Release | : 2003 |
Genre | : Eukaryotic cells |
ISBN | : 9780879695774 |
Download Molecular Cloning Book in PDF, ePub and Kindle
Author | : Heather Miller |
Publisher | : Academic Press |
Total Pages | : 232 |
Release | : 2011-10-18 |
Genre | : Science |
ISBN | : 0123855454 |
Download Molecular Biology Techniques Book in PDF, ePub and Kindle
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Author | : National Research Council |
Publisher | : National Academies Press |
Total Pages | : 128 |
Release | : 1988-01-01 |
Genre | : Science |
ISBN | : 0309038405 |
Download Mapping and Sequencing the Human Genome Book in PDF, ePub and Kindle
There is growing enthusiasm in the scientific community about the prospect of mapping and sequencing the human genome, a monumental project that will have far-reaching consequences for medicine, biology, technology, and other fields. But how will such an effort be organized and funded? How will we develop the new technologies that are needed? What new legal, social, and ethical questions will be raised? Mapping and Sequencing the Human Genome is a blueprint for this proposed project. The authors offer a highly readable explanation of the technical aspects of genetic mapping and sequencing, and they recommend specific interim and long-range research goals, organizational strategies, and funding levels. They also outline some of the legal and social questions that might arise and urge their early consideration by policymakers.