Development Of Dna Markers And The Genetic Analysis Of Seven Populations Of The Freshwater Mussel Lampsilis Radiata Siliquoidea Bivalvia Unionidae In North Central Minnesota PDF Download

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Development of DNA Markers and the Genetic Analysis of Seven Populations of the Freshwater Mussel Lampsilis Radiata Siliquoidea (Bivalvia: Unionidae) in North Central Minnesota

Development of DNA Markers and the Genetic Analysis of Seven Populations of the Freshwater Mussel Lampsilis Radiata Siliquoidea (Bivalvia: Unionidae) in North Central Minnesota
Author: Kathryn Ann Tuxbury
Publisher:
Total Pages: 152
Release: 1997
Genre:
ISBN:

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We estimated the population sizes using three different indices: actual lake area, area of suitable mussel habitat, and a population size index based on density and habitable area estimates. There was no statistically significant correlation between indices of population size and heterozygosity for either the RAPD or repeat flanking locus. The two measures of heterozygosity were significantly correlated (r = 0.715, 0.02


Conservation Genetics of a Near Threatened Freshwater Mussel Species (Lampsilis Cardium) and Improved Prospects for Recovery

Conservation Genetics of a Near Threatened Freshwater Mussel Species (Lampsilis Cardium) and Improved Prospects for Recovery
Author: Chad D. Ferguson
Publisher:
Total Pages: 228
Release: 2009
Genre: Biodiversity
ISBN:

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Freshwater mussels (Bivalvia: Unionidae) are among the most imperiled organisms in North America. While there is an urgent need for effective conservation planning and management of these organisms, important basic biological information is lacking. This research characterizes levels of genetic diversity and population structure in the Near Threatened (IUCN) freshwater mussel Lampsilis cardium in populations from Twin Creek (southwest Ohio), Little Darby and Big Darby Creeks (central Ohio), and Little Muskingum River (eastern Ohio) and assesses the extent to which regional geological events explain population structuring. Data from the congener Lampsilis ovata from Clinch River (Tennessee) are included for comparison. To characterize these patterns, sequencing of the mtDNA gene cytochrome oxidase c subunit 1 (COI) and nuclear microsatellite genotyping over 12 loci was performed. Additionally, genotype data from both adults and individual glochidia were analyzed to examine parentage and within-population levels of relatedness across common pedigree relationships. Microsatellite data reveal weak population structuring across glaciated and unglaciated drainages and 900 total river miles. However, haplotype analysis and sequence alignment recovered deeply divergent, cryptic lineages within Lampsilis cardium consistent with ancestral introgressive hybridization with Lampsilis ovata or incomplete lineage sorting. Mito-nuclear discordance argues against ongoing hybridization, although polymorphic species are also consistent with the data, and this affirms the importance of multiple molecular markers. In addition to finding multiple paternity in single broods, a number of parent-offspring, full-sibling, and half-sibling relationships for adults and glochidia are described. Numerous instances are noted in which likely full-siblings or half-siblings were located several kilometers apart, demonstrating that DNA-based evidence can describe the spatial nature of dispersal in unionid mussels. In a first report, the likely father of three glochidia from one female's brood was identified 16.2 kilometers upstream, which suggests the possibility of long-distance transport of spermatozoa in Lampsilis cardium. Given the similarity with which Lampsilines reproduce, it is predicted that other members of this genus are also capable long-distance fertilization. If fertilization in populations of freshwater mussels is indeed not limited by the density of breeding adults, the prospects for recovery in this fauna may be better than recently imagined.


The Population Genetic Structure of Quadrula Aurea (Bivalvia: Unionidae), A Threatened Freshwater Mussel in Central Texas

The Population Genetic Structure of Quadrula Aurea (Bivalvia: Unionidae), A Threatened Freshwater Mussel in Central Texas
Author: Jeffrey A. Mabe
Publisher:
Total Pages: 148
Release: 2018
Genre: Unionidae
ISBN:

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The anthropogenic alteration of riverine ecosystems has led to declines in the abundance and diversity of freshwater mussels (Bivalvia: Unionoida) worldwide. Central Texas is home to a diverse freshwater mussel fauna including three candidates for federal listing under the Endangered Species Act. Surveys conducted over the last few decades suggest many of the endemic freshwater mussel species in Texas exist in small isolated populations that may be vulnerable to the deleterious effects of genetic diversity loss. Microsatellite primers from two closely related species were used to identify a set of genetic markers that functioned in the Golden Orb (Quadrula aurea). Microsatellite markers were then applied to document the population genetic structure of Q. aurea within and among three connected river drainages in southeastern Texas. Gene flow within existing Q. aurea populations appears high indicating little potential for genetic issues stemming from isolation and inbreeding. Two weakly divergent admixed populations were identified occupying the San Antonio and Guadalupe/San Marcos rivers. Population genetic structure was related to river basin affiliation, but results for environmental factors were unresolved. Current effective population size estimates are large for the Guadalupe/San Marcos drainage and moderately large for the San Antonio drainage and there is no clear genetic evidence of contemporary population declines. Transport in the glochidial phase by a highly mobile host fish, the channel catfish (Ictalurus punctatus), may provide a mechanism for maintaining connectivity among spatially discrete mussel beds and deserves further study. Information on the occurrence and habitat associations of Q. aurea and two other threatened freshwater mussel species was documented. Quantification of the population genetic structure for Q. aurea provides important information needed for the management of this species, a baseline for understanding future changes, and insight into the factors that shape the population genetic structure of other threatened unionids in Texas.


The Genetic Structure of Remnant Fatmucket Mussel (Lampsilis Siliquoidea) Populations in the St. Clair River Delta and Surrounding Tributaries Following the Invasion of Dreissenid Mussels

The Genetic Structure of Remnant Fatmucket Mussel (Lampsilis Siliquoidea) Populations in the St. Clair River Delta and Surrounding Tributaries Following the Invasion of Dreissenid Mussels
Author: Matthew Thomas Rowe
Publisher:
Total Pages: 66
Release: 2012
Genre: Lampsilis
ISBN:

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An Evaluation of Hemolymph Extraction as a Non-lethal Sampling Method for Genetic Identification of Freshwater Mussel Species in Southeastern North Carolina

An Evaluation of Hemolymph Extraction as a Non-lethal Sampling Method for Genetic Identification of Freshwater Mussel Species in Southeastern North Carolina
Author: Michael A. McCartney
Publisher:
Total Pages: 42
Release: 2007
Genre: DNA fingerprinting of animals
ISBN:

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Identification of freshwater mussel species and subpopulations is challenging due to the plasticity of morphological characters. Molecular analyses are a powerful alternative for systematics and for biological surveys and restoration efforts. Yet most molecular methods require sacrificing animals for tissue sampling, an approach that cannot be applied to threatened and endangered species. This study demonstrated, through field trials with 3 endemic yet abundant Lake Waccamaw mussel species, that hemolymph extraction produced no mortality in animals in their native environment. The authors developed extraction methods that make hemolymph a reliable source for DNA analysis that, despite lower DNA yield, shows percent success rate in polymerase chain reaction (PCR) assays that rivals that of body tissues from sacrificed animals. The authors followed sampled and control mussels in field enclosures in Lake Waccamaw for 8 weeks and observed no mortality of either treatment group. A small, statistically not significant reduction in growth (5 - 8%) was observed in the hemolymph-removal group. The authors report the development and application of PCR assays from two mitochondrial DNA (mtDNA) regions, followed with digestion by restriction enzymes that reveal species-diagnostic DNA restriction fragment length polymorphisms (RFLP). The results are rapid and inexpensive PCRRFLP assays to discriminate among sibling species in North Carolina that are difficult and sometimes misidentified in the field, even by experienced biologists. Their regional sampling and phylogenetic analysis of mtDNAs indicates that the major taxa in Lake Waccamaw are not strictly endemic to the Lake. Samples collected in the Lake showed mtDNA sequences that were identical or very similar to sequences obtained from other rivers within the Yadkin-PeeDee and Lumber drainages. Lake Waccamaw Elliptio wacamawensis shared haplotypes with, and/or fell within a clade of, mussels identified as E. congaraea and E. wacamawensis from the Waccamaw River. The same was true of Lampsilis fullerkati specimens, which were in a clade intermingled with L. radiata and L. radiata radiata specimens from the Waccamaw and Yadkin-PeeDee rivers. Additional sampling, morphological and molecular analysis, particularly of type specimens, is needed to further resolve taxonomic relationships and biogeographic ranges. A potential cryptic species, misidentified as E. complanata, and evidence for a cryptic phylogenetic species within L. radiata radiata both appeared in their collection. Together, their results validate the use of hemolymph extraction, followed by PCR-RFLP assays and targeted DNA sequencing, as a rapid, inexpensive, and reliable identification method for field biologists at NCDOT and other agencies who routinely survey NC freshwater mussel populations.


The Ecology of Freshwater Molluscs

The Ecology of Freshwater Molluscs
Author: Robert T. Dillon
Publisher: Cambridge University Press
Total Pages: 523
Release: 2000-03-09
Genre: Science
ISBN: 1139426990

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All aspects of the ecology of freshwater molluscs are discussed in this unique volume. Extensively referenced and providing a synthesis of work from the nineteenth century onwards, this book will appeal to professional ecologists, evolutionary biologists and parasitologists interested in these diverse invertebrates.


The Freshwater Mussels of Tennessee

The Freshwater Mussels of Tennessee
Author: Paul Woodburn Parmalee
Publisher:
Total Pages: 328
Release: 1998
Genre: Nature
ISBN: 9781572330139

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"The Freshwater Mussels of Tennessee . . . is indispensable to anyone, anywhere, working on this group. Parmalee and Bogan have written a work that sets the standard for future regional guides."--G. Thomas Watters, Ohio Biological Survey "The Freshwater Mussels of Tennessee documents a tremendously diverse and unique mussel fauna that is rapidly being destroyed by modern development. Parmalee and Bogan set a new standard for state mussel surveys in their authoritative, thorough, and and highly readable account. The book will be of interest to biologists and conservationists worldwide and will appeal to anyone who cares about the preservation of natural resources in the southeastern United States."--Robert E. Warren, Illinois State Museum With more than 150 species and subspecies recorded in the state, Tennessee has one of the most diverse freshwater mussel faunas in North America. Valuable as indicators of water quality, these mollusks have themselves become threatened as development encroaches on habitat--twenty-three are currently listed as endangered species and at least twelve have become extinct. This is the first book for Tennessee to deal with this biologically and commercially significant group of mollusks. Its authors have been studying and writing about the mussels of Tennessee for more than twenty years and have undertaken a systematic organization of a large and complex body of information to bring order to a difficult field. The book traces the long history of human exploitation of mussels, from aboriginal food gathering to the growth of the cultured pearl industry. It provides an interpretive context for its exhaustive species accounts with background material on biology, distribution, economic utilization, taxonomy, and conservation issues. The authors also review the life cycle of the mussel and describe its many remarkable traits, such as its shell formation and the strategies it employs during the larval stage in parasitizing fish. The species accounts comprise 128 members of Family Unionidae--from pigtoes and pocketbooks to lilliputs and spikes--plus four additional species. The authors cover classification and synonymy, range and distribution, life history and ecology, and survival status. Particular attention is paid to shell description and structure to assist the reader in identification. Each species account includes a distribution map and color photos of two specimens. The Freshwater Mussels of Tennessee is a major reference that encompasses historical and modern mussel collections and draws on conservation studies that span two centuries. It will stand as an authoritative guide to understanding Tennessee mollusks and as a benchmark in the study of these species worldwide. The Authors: Paul W. Parmalee is professor emeritus of zooarchaeology and director emeritus of the McClung Museum at the University of Tennessee, Knoxville. Arthur E. Bogan is curator of aquatic invertebrates at the North Carolina State Museum of Natural Sciences, Raleigh.


North American Freshwater Mussels

North American Freshwater Mussels
Author: Wendell R. Haag
Publisher: Cambridge University Press
Total Pages: 523
Release: 2012-08-27
Genre: Nature
ISBN: 0521199387

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Synthesizes the ecology and natural history of North American freshwater mussels for scientists, natural resource professionals, students and natural history enthusiasts.


Quagga and Zebra Mussels

Quagga and Zebra Mussels
Author: Thomas F. Nalepa
Publisher: CRC Press
Total Pages: 820
Release: 2013-10-25
Genre: Nature
ISBN: 143985436X

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The introduction and rapid spread of two Eurasian mussel species, Dreissena polymorpha (zebra mussel) and Dreissena rostriformis bugensis (quagga mussel), in waters of North America has caused great concern among industrial and recreational water users. These invasive species can create substantial problems for raw water users such as water treatment facilities and power plants, and they can have other negative impacts by altering aquatic environments. In the 20 years since the first edition of this book was published, zebra mussels have continued to spread, and quagga mussels have become the greater threat in the Great Lakes, in deep regions of large lakes, and in the southwestern Unites States. Quagga mussels have also expanded greatly in eastern and western Europe since the first book edition was published. Quagga and Zebra Mussels: Biology, Impacts, and Control, Second Edition provides a broad view of the zebra/quagga mussel issue, offering a historic perspective and up-to-date information on mussel research. Comprising 48 chapters, this second edition includes reviews of mussel morphology, physiology, and behavior. It details mussel distribution and spread in Europe and across North America, and examines policy and regulatory responses, management strategies, and mitigation efforts. In addition, this book provides extensive coverage of the impact of invasive mussel species on freshwater ecosystems, including effects on water clarity, phytoplankton, water quality, food web changes, and consequences to other aquatic fauna. It also reviews and offers new insights on how zebra and quagga mussels respond and adapt to varying environmental conditions. This new edition includes seven video clips that complement chapter text and, through visual documentation, provide a greater understanding of mussel behavior and distribution.