Development And Utilization Of Shear Mode Acoustic Wave Biosensors For The Detection Of Ovarian Cancer PDF Download
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| Author | : Marwan Saoud |
| Publisher | : |
| Total Pages | : 198 |
| Release | : 2010 |
| Genre | : |
| ISBN | : |
Download Development and Utilization of Shear Mode Acoustic Wave Biosensors for the Detection of Ovarian Cancer Book in PDF, ePub and Kindle
Recent proteome studies have discovered the presence of heat shock protein 10 (HSP- 10) as an immunosuppressant in ovarian cancer patients. Due to the severity of ovarian cancer, the development of highly sensitive techniques for the early detection of this cancer is well in demand. In this manuscript, the thickness shear mode (TSM) acoustic wave biosensor will be used for the real-time and label-free detection of HSP-10 in buffer. The TSM sensitivity for HSP-10 is evaluated based on resonance frequency shifts generated by the biosensor. A nucleic acid aptamer, which is specifically engineered by in vitro selection to target HSP-10, is employed as the biosensing element of the biosensor. Alkylthiol-based self-assembling monolayers (SAMs), composed of various linker/diluent molar ratios, are used to immobilize the aptamer onto gold-coated piezoelectric quartz substrates. The TSM biosensing properties for avidin-biotin interactions are also evaluated in order to assess the biosensor response to HSP-10 protein-aptamer interaction.
| Author | : Christopher David Corso |
| Publisher | : |
| Total Pages | : |
| Release | : 2008 |
| Genre | : Acoustic surface wave devices |
| ISBN | : |
Download Theoretical and Experimental Development of a ZnO-based Laterally Excited Thickness Shear Mode Acoustic Wave Immunosensor for Cancer Biomarker Detection Book in PDF, ePub and Kindle
The object of this thesis research was to develop and characterize a new type of acoustic biosensor - a ZnO-based laterally excited thickness shear mode (TSM) resonator in a solidly mounted configuration. The first specific aim of the research was to develop the theoretical underpinnings of the acoustic wave propagation in ZnO. Theoretical calculations were carried out by solving the piezoelectrically stiffened Christoffel equation to elucidate the acoustic modes that are excited through lateral excitation of a ZnO stack. A finite element model was developed to confirm the calculations and investigate the electric field orientation and density for various electrode configurations. A proof of concept study was also carried out using a Quartz Crystal Microbalance device to investigate the application of thickness shear mode resonators to cancer biomarker detection in complex media. The results helped to provide a firm foundation for the design of new gravimetric sensors with enhanced capabilities. The second specific aim was to design and fabricate arrays of multiple laterally excited TSM devices and fully characterize their electrical properties. The solidly mounted resonator configuration was developed for the ZnO-based devices through theoretical calculations and experimentation. A functional mirror comprised of W and SiO2 was implemented in development of the TSM resonators. The devices were fabricated and tested for values of interest such as Q, and electromechanical coupling (K2) as well as their ability to operate in liquids.
| Author | : Adam Daniel Wathen |
| Publisher | : |
| Total Pages | : |
| Release | : 2011 |
| Genre | : Acoustic surface wave devices |
| ISBN | : |
Download Acoustic Wave Biosensor Arrays for the Simultaneous Detection of Multiple Cancer Biomarkers Book in PDF, ePub and Kindle
The analysis and development of robust sensing platforms based on solidly-mounted ZnO bulk acoustic wave devices was proposed. The exploitation of acoustic energy trapping was investigated and demonstrated as a method to define active sensing areas on a substrate. In addition, a new "hybrid" acoustic mode experiencing acoustic energy trapping was studied theoretically and experimentally. This mode was used as an explanation of historical inconsistencies in observed thickness-shear mode velocities. Initial theoretical and experimental results suggest that this mode is a coupling of thickness-shear and longitudinal particle displacements and, as such, may offer more mechanical and/or structural information about a sample under test. Device development was taken another step further and multi-mode ZnO resonators operating in the thickness-shear, hybrid, and longitudinal modes were introduced. These devices were characterized with respect to sample viscosity and conductivity and preliminary results show that, with further development, the multi-mode resonators provide significantly more information about a sample than their single-mode counterparts. An alternative to resonator-based platforms was also presented in the form of bulk acoustic delay lines. Initial conceptual and simulation results show that these devices provide a different perspective of typical sensing modalities by using properly designed input pulses, device tuning, and examining overall input and output signal spectra.
| Author | : |
| Publisher | : |
| Total Pages | : 269 |
| Release | : 2009 |
| Genre | : |
| ISBN | : |
Download Development of Highly Sensitive Bulk Acoustic Wave Device Biosensor Arrays for Screening and Early Detection of Prostate Cancer Book in PDF, ePub and Kindle
In this research, I present several novel contributions to the field of microelectronic acoustic biosensors that approach the goal of developing a cost-effective, highly sensitive and highly selective sensor array for the detection of early cancer proliferation. First I report on the development of a novel solidly mounted shear-mode resonator employing piezoelectric ZnO as an appropriate base device for liquid-phase sensing applications. Second I report on the development of a novel and appropriate chemical surface preparation protocol for the covalent immobilization of monoclonal IgG antibodies to the surface of this device, thereby functionalizing it as a biosensor. Proof of functionality is demonstrated employing these sensors for the detection of various known cancer biomarkers in complex media as well as progress towards developing a packaged disposable sensor array system is also reported.
| Author | : Anthony Neal Watkins |
| Publisher | : |
| Total Pages | : 420 |
| Release | : 1996 |
| Genre | : Automatic gain control |
| ISBN | : |
Download A General Approach to the Development and Application of a Biosensor Based on the Thickness Shear Mode Acoustic Sensor Book in PDF, ePub and Kindle
| Author | : Andrew Paul Campitelli |
| Publisher | : |
| Total Pages | : 506 |
| Release | : 1997 |
| Genre | : Biosensors |
| ISBN | : |
Download Investigation of a Shear Horizontal Surface Acoustic Wave Sensor System for Liquid Media Book in PDF, ePub and Kindle
| Author | : Chi-Jung Cheng |
| Publisher | : |
| Total Pages | : 117 |
| Release | : 2012 |
| Genre | : |
| ISBN | : |
Download Design, Modeling and Fabrication of Shear Mode Bulk Acoustic Wave Sensor as a Potential Biosensor Book in PDF, ePub and Kindle
There has been an increasing interest in development of thin film bulk acoustic wave resonator devices for chemical and biological sensing applications in the environmental and biomedical industries. The zinc oxide thin film FBAR devices provide attractive advantages, such as high resonant frequency, small size, and rapid response. Typically, the ZnO-based FBAR devices are operated in the longitudinal wave mode where the ZnO crystallites are perpendicular to the substrate. However, the longitudinal wave mode is adversely affected when used in liquid environments. To overcome this limitation, the shear mode solidly mounted film bulk acoustic wave resonator device is presented in this dissertation.
| Author | : Victor Serban Crivianu-Gaita |
| Publisher | : |
| Total Pages | : |
| Release | : 2016 |
| Genre | : |
| ISBN | : |
Download Acoustic Wave Biosensor for the Detection of the Breast Cancer Metastasis Biomarker Protein PTHrP. Book in PDF, ePub and Kindle
| Author | : Desmond Dion Stubbs |
| Publisher | : |
| Total Pages | : |
| Release | : 2005 |
| Genre | : Biosensors |
| ISBN | : |
Download Development of an Acoustic Wave Based Biosensor for Vapor Phase Detection of Small Molecules Book in PDF, ePub and Kindle
For centuries scientific ingenuity and innovation have been influenced by Mother Natures perfect design. One of her more elusive designs is that of the sensory olfactory system, an array of highly sensitive receptors responsible for chemical vapor recognition. In the animal kingdom this ability is magnified among canines where ppt (parts per trillion) sensitivity values have been reported. Today, detection dogs are considered an essential part of the US drug and explosives detection schemes. However, growing concerns about their susceptibility to extraneous odors have inspired the development of highly sensitive analytical detection tools or biosensors known as electronic noses. In general, biosensors are distinguished from chemical sensors in that they use an entity of biological origin (e.g. antibody, cell, enzyme) immobilized onto a surface as the chemically-sensitive film on the device. The colloquial view is that the term biosensors refers to devices which detect the presence of entities of biological origin, such as proteins or single-stranded DNA and that this detection must take place in a liquid. Our biosensor utilizes biomolecules, specifically IgG monoclonal antibodies, to achieve molecular recognition of relatively small molecules in the vapor phase.
| Author | : Keith E. Herold |
| Publisher | : Taylor & Francis |
| Total Pages | : 841 |
| Release | : 2012-05-29 |
| Genre | : Medical |
| ISBN | : 1439841667 |
Download Biosensors and Molecular Technologies for Cancer Diagnostics Book in PDF, ePub and Kindle
Bridging the gap between research and clinical application, Biosensors and Molecular Technologies for Cancer Diagnostics explores the use of biosensors as effective alternatives to the current standard methods in cancer diagnosis and detection. It describes the major aspects involved in detecting and diagnosing cancer as well as the basic elements
