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Genome Engineering via CRISPR-Cas9 System

Genome Engineering via CRISPR-Cas9 System
Author: Vijai Singh
Publisher: Academic Press
Total Pages: 0
Release: 2020-02-21
Genre: Science
ISBN: 9780128181409
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Genome Engineering via CRISPR-Cas9 Systems presents a compilation of chapters from eminent scientists from across the globe who have established expertise in working with CRISPR-Cas9 systems. Currently, targeted genome engineering is a key technology for basic science, biomedical and industrial applications due to the relative simplicity to which they can be designed, used and applied. However, it is not easy to find relevant information gathered in a single source. The book contains a wide range of applications of CRISPR in research of bacteria, virus, algae, plant and mammalian and also discusses the modeling of drosophila, zebra fish and protozoan, among others. Other topics covered include diagnosis, sensor and therapeutic applications, as well as ethical and regulatory issues. This book is a valuable source not only for beginners in genome engineering, but also researchers, clinicians, stakeholders, policy makers, and practitioners interested in the potential of CRISPR-Cas9 in several fields.

CRISPR-Cas Systems

CRISPR-Cas Systems
Author: Rodolphe Barrangou
Publisher: Springer Science & Business Media
Total Pages: 300
Release: 2012-12-13
Genre: Science
ISBN: 364234657X
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CRISPR/Cas is a recently described defense system that protects bacteria and archaea against invasion by mobile genetic elements such as viruses and plasmids. A wide spectrum of distinct CRISPR/Cas systems has been identified in at least half of the available prokaryotic genomes. On-going structural and functional analyses have resulted in a far greater insight into the functions and possible applications of these systems, although many secrets remain to be discovered. In this book, experts summarize the state of the art in this exciting field.

The CRISPR/Cas System

The CRISPR/Cas System
Author: Muhammad Jamal
Publisher: Caister Academic Press Limited
Total Pages: 0
Release: 2017
Genre: Medical
ISBN: 9781910190630
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The use of CRISPR/Cas technology for genome editing suggests many potential applications, including the alteration of the germline of humans, animals and food crops. The speed and efficiency of the CRISPR/Cas system make it a potentially useful system for gene therapy. In this volume expert international authors provide a useful and timely review of the applications of the CRISPR/Cas system across diverse fields and explore further avenues and research directions of this novel and powerful editing technology. The technology and its application are reviewed with respect to reproduction and development, immunity and genetic diseases, system structure and system specificity. Some of the potential problems of the CRISPR/Cas system are also discussed, in particular the specificity of the system: this remains an important topic as improvement could lead to the more direct and efficient use of the CRISPR/Cas system in clinical settings. The authors also debate ethical concerns associated with this powerful new technology. This volume is a rigorous review of the applications and new opportunities for the CRISPR/Cas system and provides a stimulus for current and future research. An invaluable guide for all scientists working in the fields of genome editing and gene therapy the book is also recommended for all life sciences libraries.

Plant Genome Editing with CRISPR Systems

Plant Genome Editing with CRISPR Systems
Author: Yiping Qi
Publisher: Springer Nature
Total Pages: 360
Release: 2019-01-04
Genre: Science
ISBN: 149398991X
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This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.

Reprogramming the Genome: Applications of CRISPR-Cas in non-mammalian systems part A

Reprogramming the Genome: Applications of CRISPR-Cas in non-mammalian systems part A
Author:
Publisher: Academic Press
Total Pages: 244
Release: 2021-03-28
Genre: Science
ISBN: 0323853226
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Reprogramming the Genome: Applications of CRISPR-Cas in Non-mammalian Systems, Part A presents a collation of chapters written by global, eminent scientists. CRISPR-Cas9 system is an RNA-mediated immune system of bacteria and archaea that protects from bacteriophage infections. It is one of the revolutionized technologies to uplift biology to the next stages. Chapters in this release include An Introduction and applications of CRISPR-Cas Systems, History, evolution and classification of CRISPR-Cas associated systems, CRISPR based bacterial genome editing and removal of pathogens, CRISPR based genome editing and removal of human viruses, CRISPR based development of RNA editing and diagnostic platform, and much more. Additional sections cover Genome engineering in insects for control of vector borne diseases, Development of insect cell line using CRISPR technology, CRISPRing protozoan parasites to better understand the biology of diseases, CRISPR based genome editing of Caenorhabditis elegans, and a variety of other important topics. Offers a basic understanding and clear picture of genome editing CRISPR-Cas systems in different organisms Explains how to create an animal model for disease diagnosis/research and reprogram CRISPR for removal of virus, bacteria, fungi, protozoan, and many more Discusses the advances, patents, applications, challenges and opportunities in CRISPR-Cas9 systems in basic sciences, biomedicine, virology, bacteriology, molecular biology, and many more

Reprogramming the Genome: Applications of CRISPR-Cas in non-mammalian systems part B

Reprogramming the Genome: Applications of CRISPR-Cas in non-mammalian systems part B
Author:
Publisher: Academic Press
Total Pages: 200
Release: 2021-04-29
Genre: Science
ISBN: 032385298X
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Reprogramming the Genome: Applications of CRISPR-Cas in Non-mammalian Systems Part B, represents the collation of chapters written by eminent scientists worldwide. CRISPR-Cas9 system is an RNA-mediated immune system of bacteria and archaea that protects from bacteriophage infections. It is one of the revolutionized technologies to uplift biology to the next stages. It is a simple, rapid, precise, and cost-effective tool for genome editing and regulation of a wide range of organisms. It has gained scientific and public attention worldwide. This volume mainly covers insect cell line, protozoans, zebrafish, drosophila, CRISPRi, patents as well as technology transfer, and many more. This book is a key source of information available in a single volume. This book will be useful for not only beginners in genome engineering, but also students, researchers, scientists, policymakers, and stakeholders interested in harnessing the potential of reprogramming of the genomes in several areas. Offers basic understanding and a clear picture of genome editing CRISPR-Cas systems in different organisms Explains how to create an animal model for disease diagnosis/research and reprogram CRISPR for insect cell line, protozoans, zebrafish, drosophila, and many more Discusses the advances, patents, applications, challenges and opportunities in CRISPR-Cas9 systems in basic sciences, biomedicine, molecular biology and many more

CRISPR-Cas System in Translational Biotechnology

CRISPR-Cas System in Translational Biotechnology
Author: Swati Joshi
Publisher: Elsevier
Total Pages: 317
Release: 2023-12-01
Genre: Science
ISBN: 0323972284
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CRISPR-Cas System in Translational Biotechnology discusses applied and translational aspects of the CRISPR-Cas technology. The book bridges the gap between theoretical knowledge and practical solutions surrounding this emerging and impactful technology in several academic and industrial fields. It is split in five sections: CRISP-Cas fundamentals and advancements; CRISP-Cas in medical biotechnology; CRISP-Cas in environmental biotechnology; CRISP-Cas in food biotechnology; and biosafety, patents and commercialization of CRISP-Cas technology. Written by experts from diverse backgrounds, the content covers the subject and its impact in multiple fields. It is a valuable resource for graduate students and researchers on bioinformatics, systems biology, and members of the biomedical field and biotechnology industry who are interested in learning more about CRISP-Cas system and its applications. Discusses applied aspects of CRISPR-Cas technology and state-of-the-art technological/translational advancements in the field Focuses on the CRISPR-Cas mediated genetic engineering for employment in various industries such as medical, agricultural, environmental and food Encompasses knowledge on CRISPR-Cas commercialization, potential markets and associated ethical challenges

Advances in CRISPR/Cas and Related Technologies

Advances in CRISPR/Cas and Related Technologies
Author: Dipanjan Ghosh
Publisher: Academic Press
Total Pages: 284
Release: 2021-03-05
Genre: Science
ISBN: 0128215917
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Advances in CRISPR/Cas and related technologies, Volume 179, the latest release in this ongoing series, deals with a wide variety of research topics related to recent advancement in the genome editing techniques. Associated chapters in this new release include Challenges for Therapeutic application of CRISPR Cas techniques, Mitochondrial DNA modification by CRISPR Cas System: Challenges and future direction, Trends in CRISPR Cas technology application in cancer, Modified CRISPR-Cas for next generation application, Application of CRISPR Cas in Synthetic Biology: Challenges and Scopes, History of CRISPR Cas system from bacterial Adaptive Immune System to research application, and more. Covers the Cas9 protein modification for reduced off-target effect Includes discussions on Cas9 utilization for Metabolic Engineering Provides information on the use of Cas9 for targeted delivery in therapeutic application

CRISPR-Cas Enzymes

CRISPR-Cas Enzymes
Author:
Publisher: Academic Press
Total Pages: 451
Release: 2019-01-25
Genre: Science
ISBN: 0128167610
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CRISPR-Cas Enzymes, Volume 616, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics covered in this release include CRISPR bioinformatics, A method for one-step assembly of Class 2 CRISPR arrays, Biochemical reconstitution and structural analysis of ribonucleoprotein complexes in Type I-E CRISPR-Cas systems, Mechanistic dissection of the CRISPR interference pathway in Type I-E CRISPR-Cas system, Site-specific fluorescent labeling of individual proteins within CRISPR complexes, Fluorescence-based methods for measuring target interference by CRISPR-Cas systems, Native State Structural Characterization of CRISRP Associated Complexes using Mass Spectrometry, and more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Updated release includes the latest information on the CRISPR-Cas Enzymes

CRISPR

CRISPR
Author: Magnus Lundgren
Publisher: Humana
Total Pages: 0
Release: 2016-10-09
Genre: Medical
ISBN: 9781493954285
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This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CRISPR: Methods and Protocols provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems.