Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Coherent Anti Stokes Raman Scattering Second Harmonic Generation And Two Photon Excitation Fluorescence Multimodal Microscope PDF full book. Access full book title Coherent Anti Stokes Raman Scattering Second Harmonic Generation And Two Photon Excitation Fluorescence Multimodal Microscope.
| Author | : Leonardo Michael Mortati |
| Publisher | : |
| Total Pages | : |
| Release | : 2013 |
| Genre | : |
| ISBN | : |
| Author | : Huda Yousif |
| Publisher | : |
| Total Pages | : |
| Release | : 2018 |
| Genre | : |
| ISBN | : |
Coherent anti-Stokes Raman scattering (CARS) microscopy is considered as a powerful tool for non-invasive chemical imaging of biological samples. CARS microscopy provides an endogenous contrast mechanism that it is sensitive to molecular vibrations. CARS microscopy is recognized as a great imaging system, especially in vivo experiments since it eliminates the need for the contrast agents. In this thesis, CARS microscopy/spectroscopy is built from scratch by employing a single (Ti-Sapphire) laser source generating 65 femtosecond laser pulses centered at 800 nm wavelength. Two closely lying zero dispersion photonic crystal fiber (PCF) is used to generate the supercontinuum for the Stokes beam to generate CARS at 2885 cm-1 to match lipids rich vibrational frequency. XY galvanometers are used for laser raster scanning across the sample. The initial generation of CARS signal was in the forward direction. After guaranteeing a strong CARS signal, images for chemical and biological samples were taken. To achieve a multimodal imaging technique, CARS microscopy imaging system is combined with two- photon excitation fluorescent (TPEF) and second harmonic generation (SHG) imaging techniques, where various information was extracted from the imaged samples. Images with our CARS microscopy show a good resolution and sensitivity. The second part of my work is to reduce the footprint for this setup to make it more suitable for use in clinical applications. For that reason, I integrated a homebuilt endoscope and all fiber femtosecond laser source together to get a fiber based imaging system. Proof of principal for the integrated system is achieved by obtaining a reasonable agreement in accuracy and resolution to those obtained by the endoscope driven by Ti-sapphire laser.
| Author | : Francesco S. Pavone |
| Publisher | : Taylor & Francis |
| Total Pages | : 465 |
| Release | : 2016-04-19 |
| Genre | : Science |
| ISBN | : 1439849153 |
Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical a
| Author | : Ji-Xin Cheng |
| Publisher | : Elsevier |
| Total Pages | : 612 |
| Release | : 2021-12-04 |
| Genre | : Science |
| ISBN | : 0323903371 |
Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics
| Author | : Ji-Xin Cheng |
| Publisher | : CRC Press |
| Total Pages | : 603 |
| Release | : 2016-04-19 |
| Genre | : Science |
| ISBN | : 1439867666 |
The First Book on CRS MicroscopyCompared to conventional Raman microscopy, coherent Raman scattering (CRS) allows label-free imaging of living cells and tissues at video rate by enhancing the weak Raman signal through nonlinear excitation. Edited by pioneers in the field and with contributions from a distinguished team of experts, Coherent Raman Sc
| Author | : M.S. Feld |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 390 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 3642814956 |
| Author | : Alecsandru Ioan Baba |
| Publisher | : |
| Total Pages | : 787 |
| Release | : 2007 |
| Genre | : Electronic books |
| ISBN | : 9789732714577 |
| Author | : V. S. Letokhov |
| Publisher | : Springer |
| Total Pages | : 466 |
| Release | : 2014-04-18 |
| Genre | : Technology & Engineering |
| ISBN | : 9783662134870 |
The laser as a source of coherent optical radiation has made it possible to investigate nonlinear interaction of optical radiation with atoms and mole cules. Its availability has given rise to new research fields, such as non linear optics, laser spectroscopy, laser photochemistry, that lie at the boundary between quantum electronics and physical optics, optical spectros copy and photochemistry, respectively. The use of coherent optical radiation in each of these fields has led to the discovery of qualitatively ne\~ effects and possibilities; in particular, some rather subtle effects of interaction between highly monochromatic light and atoms and molecules, in optical spec troscopy, have formed the bases for certain methods of so-called nonlinear, laser Doppler-free spectroscopy. These methods have made it possible to in 5 6 crease the resolution of spectroscopic studies from between 10 and 10 , lim 11 ited by Doppl er 1 i ne broadeni ng up, to about 10 ; at present some 1 abor atories are developing new techniques that have even higher resolution. The discovery and elaboration of the methods of nonlinear laser spectroscopy have resulted largely from contributions by scientists from many countries, in particular from the USA (Massachusetts Institute of Technology, Stanford Uni versity, National Bureau of Standards in Boulder, Harvard University, etc. ), the USSR (P. N. Levedev Institute of Physics, Institute of Semiconductor Phys ics in Novosibirsk, Institute of Spectroscopy, etc.
| Author | : National Research Council |
| Publisher | : National Academies Press |
| Total Pages | : 222 |
| Release | : 2006-06-01 |
| Genre | : Science |
| ISBN | : 030916463X |
Scientists and engineers have long relied on the power of imaging techniques to help see objects invisible to the naked eye, and thus, to advance scientific knowledge. These experts are constantly pushing the limits of technology in pursuit of chemical imagingâ€"the ability to visualize molecular structures and chemical composition in time and space as actual events unfoldâ€"from the smallest dimension of a biological system to the widest expanse of a distant galaxy. Chemical imaging has a variety of applications for almost every facet of our daily lives, ranging from medical diagnosis and treatment to the study and design of material properties in new products. In addition to highlighting advances in chemical imaging that could have the greatest impact on critical problems in science and technology, Visualizing Chemistry reviews the current state of chemical imaging technology, identifies promising future developments and their applications, and suggests a research and educational agenda to enable breakthrough improvements.
| Author | : Y. R. Shen |
| Publisher | : Wiley-Interscience |
| Total Pages | : 596 |
| Release | : 2003 |
| Genre | : Science |
| ISBN | : |
A comprehensive treatment of nonlinear optics emphasizing physical concepts and the relationhip between theory and experiment. Systematically describes a number of sub-topics in the field. Up-to-date references and numerous illustrations will help both beginners and practitioners interested in gaining a more thorough understanding of the subject.
