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| Author | : Na Hui Kim |
| Publisher | : |
| Total Pages | : 246 |
| Release | : 2008 |
| Genre | : |
| ISBN | : |
| Author | : David Kinahan |
| Publisher | : MDPI |
| Total Pages | : 123 |
| Release | : 2021-09-06 |
| Genre | : Technology & Engineering |
| ISBN | : 3036513663 |
Microfluidics and lab-on-a-chip have, in recent years, come to the forefront in diagnostics and detection. At point-of-care, in the emergency room, and at the hospital bed or GP clinic, lab-on-a-chip offers the potential to rapidly detect time-critical and life-threatening diseases such as sepsis and bacterial meningitis. Furthermore, portable and user-friendly diagnostic platforms can enable disease diagnostics and detection in resource-poor settings where centralised laboratory facilities may not be available. At point-of-use, microfluidics and lab-on-chip can be applied in the field to rapidly identify plant pathogens, thus reducing the need for damaging broad spectrum pesticides while also reducing food losses. Microfluidics can also be applied to the continuous monitoring of water quality and can support policy-makers and protection agencies in protecting the environment. Perhaps most excitingly, microfluidics also offers the potential to enable entirely new diagnostic tests that cannot be implemented using conventional laboratory tools. Examples of microfluidics at the frontier of new medical diagnostic tests include early detection of cancers through circulating tumour cells (CTCs) and highly sensitive genetic tests using droplet-based digital PCR. This Special Issue on “Advances in Microfluidics Technology for Diagnostics and Detection” aims to gather outstanding research and to carry out comprehensive coverage of all aspects related to microfluidics in diagnostics and detection.
| Author | : Chandra K. Dixit |
| Publisher | : Springer |
| Total Pages | : 256 |
| Release | : 2016-10-13 |
| Genre | : Medical |
| ISBN | : 3319400363 |
This book describes novel microtechnologies and integration strategies for developing a new class of assay systems to retrieve desired health information from patients in real-time. The selection and integration of sensor components and operational parameters for developing point-of-care (POC) are also described in detail. The basics that govern the microfluidic regimen and the techniques and methods currently employed for fabricating microfluidic systems and integrating biosensors are thoroughly covered. This book also describes the application of microfluidics in the field of cell and molecular biology, single cell biology, disease diagnostics, as well as the commercially available systems that have been either introduced or have the potential of being used in research and development. This is an ideal book for aiding biologists in understanding the fundamentals and applications of microfluidics. This book also: Describes the preparatory methods for developing 3-dimensional microfluidic structures and their use for Lab-on-a-Chip design Explains the significance of miniaturization and integration of sensing components to develop wearable sensors for point-of-care (POC) Demonstrates the application of microfluidics to life sciences and analytical chemistry, including disease diagnostics and separations Motivates new ideas related to novel platforms, valving technology, miniaturized transduction methods, and device integration to develop next generation sequencing Discusses future prospects and challenges of the field of microfluidics in the areas of life sciences in general and diagnostics in particular
| Author | : Mary Amasia |
| Publisher | : |
| Total Pages | : 181 |
| Release | : 2012 |
| Genre | : |
| ISBN | : 9781267157911 |
Microfluidic diagnostic systems are positioned to revolutionize healthcare by enabling the miniaturization and integration of complex diagnostic analyses into platforms that can deliver fast, low-cost diagnostic results and minimize the burden at the point-of-care, serving populations at sites that are remote, resource-limited, and lack access to large hospital laboratory technologies and specialized medical technicians. In order to be successful, the design of novel platforms for microfluidic diagnostics must therefore attend to real-world scenarios of use and consider sample-to-answer viability. Arguably one of the greatest challenges for point-of-care microfluidic diagnostics is sample preparation. While a rich research stream has pushed the frontiers of microfluidic systems, demonstrating their viability in achieving rapid results with miniscule samples, the real-world sample concerns have received little attention. These sample-related properties present unique challenges to the design of microfluidic diagnostic systems and are vital to the success of point-of-care diagnostics. This dissertation contributes to the emerging field of point-of-care microfluidic diagnostic systems through the development of two novel centrifugal microfluidic platforms that address the significant challenges of real-world sample properties. It provides a detailed account of the design of two integrated microfluidic systems: 1) a blood sample preparation disc and 2) an automated PCR analysis disc. Together these two systems provide a case for sample-driven design by addressing and overcoming significant sample-related challenges. The first disc design addresses the unique requirements of a large-volume sample and the second highlights engineering problems associated with low-concentration samples. The dissertation presents work in the design and development of these systems and explores fundamental engineering implications of sample-driven design. It first presents the motivation for sample-to-answer nucleic acid diagnostic systems and the need for attention to sample-driven design concerns by exploring the benefits and challenges of adapting diagnostic systems onto point-of-care microfluidic platforms. Background information on the history and current state-of-the-art in compact-disc microfluidics technologies, practices and theory is provided. Next, the motivation for sample-driven design is discussed in further depth through its application to large-volume and low-concentration sample device design including in-depth modeling of CD microfluidic behavior as it pertains to uniform filling of and dynamic temperature distributions within a microchamber. Novel research is then presented on 1) a centrifugal microfluidic platform for large-volume blood sample preparation and 2) a centrifugal microfluidic platform for real-time PCR amplification for low-concentration nucleic acid sample detection. Finally, an outlook on the future of point-of-care microfluidic systems is discussed along with future work needed to fully realize the vision for the systems presented here.
| Author | : Raju Khan |
| Publisher | : CRC Press |
| Total Pages | : 416 |
| Release | : 2021-03-11 |
| Genre | : Medical |
| ISBN | : 1000548376 |
This book provides a well-focused and comprehensive overview of novel technologies involved in advanced microfluidics based diagnosis via various types of prognostic and diagnostic biomarkers. This authors examine microfluidics based diagnosis in the biomedical field as an upcoming field with extensive applications. It provides a unique approach and comprehensive technology overview for diagnosis management towards early stages of various bioanalytes via cancer diagnostics diabetes, alzheimer disease, toxicity in food products, brain and retinal diseases, cardiovascular diseases, and bacterial infections etc. Thus, this book would encompass a combinatorial approach of medical science, engineering and biomedical technology. The authors provide a well-focused and comprehensive overview of novel technologies involved in advanced microfluidics based diagnosis via various types of prognostic and diagnostic biomarkers. Moreover, this book contains detailed description on the diagnosis of novel techniques. This book would serve as a guide for students, scientists, researchers, and microfluidics based point of care technologies via smart diagnostics and to plan future research in this valuable field.
| Author | : Utkan Demirci |
| Publisher | : World Scientific |
| Total Pages | : 495 |
| Release | : 2012 |
| Genre | : Medical |
| ISBN | : 9814405515 |
Ch. 1. A microscale bioinspired cochlear-like sensor / Robert D. White, Robert Littrell, and Karl Grosh -- ch. 2. Systematic evaluation of the efficiencies of proteins and chemicals in pharmaceutical applications / Morgan Hamon and Jong Wook Hong -- ch. 3. Microfluidic glucose sensors / Jithesh V. Veetil [und weitere] -- ch. 4. Applications of microfabrication and microfluidic techniques in mesenchymal stem cell research / Abhijit Majumder [und weitere] -- ch. 5. Patient-specific modeling of low-density lipoprotein transport in coronary arteries / Ufuk Olgac -- ch. 6. Point-of-care microdevices for global health diagnostics of infectious diseases / Sau Yin Chin [und weitere] -- ch. 7. Integrated microfluidic sample preparation for chip-based molecular diagnostics / Jane Y. Zhang [und weitere] -- ch. 8. Microfluidic devices for cellular proteomic studies / Yihong Zhan and Chang Lu -- ch. 9. Microfluidics for neuroscience: novel tools and future implications / Vivian M. Hernandez and P. Hande Ozdinler -- ch. 10. Microfluidics: on-chip platforms as in vitro disease models / Shan Gao, Erkin Seker, and Martin L. Yarmush -- ch. 11. Application of microfluidics in stem cell and tissue engineering / Sasha H. Bakhru, Christopher Highley, and Stefan Zappe -- ch. 12. Microfluidic "on-the-fly" fabrication of microstructures for biomedical applications / Edward Kang, Sau Fung Wong, and Sang-Hoon Lee -- ch. 13. Microfluidics as a promising tool toward distributed viral detection / Elodie Sollier and Dino Di Carlo -- ch. 14. Electrophoresis and dielectrophoresis for lab-on-a-chip (LOC) analyses / Yagmur Demircan, Gurkan Yilmaz, and Haluk Kulah -- ch. 15. Ultrasonic embossing of carbon nanotubes for the fabrication of polymer microfluidic chips for DNA sample purification / Puttachat Khuntontong, Min Gong, and Zhiping Wang -- ch. 16. Ferrofluidics / A. Rezzan Kose and Hur Koser -- ch. 17. Antibody-based blood bioparticle capture and separation using microfluidics for global health / ZhengYuan Luo [und weitere] -- ch. 18. Applications of quantum dots for fluorescence imaging in biomedical research / ShuQi Wang [und weitere]
| Author | : Mahdi Mohammadi |
| Publisher | : |
| Total Pages | : |
| Release | : 2016 |
| Genre | : Science |
| ISBN | : |
Since the 1990s, centrifugal microfluidic platforms have evolved into a mature technology for the automation of bioanalytical assays in decentralized settings. These "Lab-on-a-Disc" (LoaD) systems have already implemented a range of laboratory unit operations (LUOs) such as sample loading, liquid transport, metering, aliquoting, routing, mixing, and washing. By assembling these LUOs in highly functional microfluidic networks, including sample preparation and detection, a sizable portfolio of common test formats such as general chemistry, immunoassays/ protein analysis, nucleic acid testing, and cell counting has been established. The availability of these bioanalytical assay types enables a broad range of applications in fields such as life-science research, biomedical point-of-care testing and veterinary diagnostics, as well as agrifood, environmental, infrastructural, and industrial monitoring.
| Author | : Mohamed Ibrahim |
| Publisher | : CRC Press |
| Total Pages | : 349 |
| Release | : 2020-05-31 |
| Genre | : Technology & Engineering |
| ISBN | : 1000082660 |
A microfluidic biochip is an engineered fluidic device that controls the flow of analytes, thereby enabling a variety of useful applications. According to recent studies, the fields that are best set to benefit from the microfluidics technology, also known as lab-on-chip technology, include forensic identification, clinical chemistry, point-of-care (PoC) diagnostics, and drug discovery. The growth in such fields has significantly amplified the impact of microfluidics technology, whose market value is forecast to grow from $4 billion in 2017 to $13.2 billion by 2023. The rapid evolution of lab-on-chip technologies opens up opportunities for new biological or chemical science areas that can be directly facilitated by sensor-based microfluidics control. For example, the digital microfluidics-based ePlex system from GenMarkDx enables automated disease diagnosis and can bring syndromic testing near patients everywhere. However, as the applications of molecular biology grow, the adoption of microfluidics in many applications has not grown at the same pace, despite the concerted effort of microfluidic systems engineers. Recent studies suggest that state-of-the-art design techniques for microfluidics have two major drawbacks that need to be addressed appropriately: (1) current lab-on-chip systems were only optimized as auxiliary components and are only suitable for sample-limited analyses; therefore, their capabilities may not cope with the requirements of contemporary molecular biology applications; (2) the integrity of these automated lab-on-chip systems and their biochemical operations are still an open question since no protection schemes were developed against adversarial contamination or result-manipulation attacks. Optimization of Trustworthy Biomolecular Quantitative Analysis Using Cyber-Physical Microfluidic Platforms provides solutions to these challenges by introducing a new design flow based on the realistic modeling of contemporary molecular biology protocols. It also presents a microfluidic security flow that provides a high-level of confidence in the integrity of such protocols. In summary, this book creates a new research field as it bridges the technical skills gap between microfluidic systems and molecular biology protocols but it is viewed from the perspective of an electronic/systems engineer.
| Author | : Valérie Taly |
| Publisher | : Springer Nature |
| Total Pages | : 254 |
| Release | : |
| Genre | : |
| ISBN | : 1071638505 |
| Author | : Chang Lu |
| Publisher | : Springer |
| Total Pages | : 382 |
| Release | : 2016-05-14 |
| Genre | : Medical |
| ISBN | : 3319300199 |
This book covers the state-of-the-art research on molecular biology assays and molecular techniques enabled or enhanced by microfluidic platforms. Topics covered include microfluidic methods for cellular separations and single cell studies, droplet-based approaches to study protein expression and forensics, and microfluidic in situ hybridization for RNA analysis. Key molecular biology studies using model organisms are reviewed in detail. This is an ideal book for students and researchers in the microfluidics and molecular biology fields as well as engineers working in the biotechnology industry. This book also: Reviews exhaustively the latest techniques for single-cell genetic, epigenetic, metabolomic, and proteomic analysis Illustrates microfluidic approaches for inverse metabolic engineering, as well as analysis of circulating exosomes Broadens readers’ understanding of microfluidics convection-based PCR technology, microfluidic RNA-seq, and microfluidics for robust mobile diagnostics
