Cell Free Protein Expression PDF Download
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Author | : Ashty S. Karim |
Publisher | : |
Total Pages | : 435 |
Release | : 2022 |
Genre | : Biology |
ISBN | : 9781071619988 |
Download Cell-Free Gene Expression Book in PDF, ePub and Kindle
This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell‐Free Gene Expression: Methods and Protocols serves as an ideal guide for researchers seeking technical methods to current aspects of CFE and related applications.
Author | : Ashty S. Karim |
Publisher | : Humana |
Total Pages | : 435 |
Release | : 2022-01-06 |
Genre | : Science |
ISBN | : 9781071619971 |
Download Cell-Free Gene Expression Book in PDF, ePub and Kindle
This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell‐Free Gene Expression: Methods and Protocols serves as an ideal guide for researchers seeking technical methods to current aspects of CFE and related applications.
Author | : James R. Swartz |
Publisher | : Springer Science & Business Media |
Total Pages | : 213 |
Release | : 2012-12-06 |
Genre | : Science |
ISBN | : 3642593372 |
Download Cell-Free Protein Expression Book in PDF, ePub and Kindle
Cell-free protein synthesis is coming of age! Motivated by an escalating need for efficient protein synthesis and empowered by readily accessible cell-free protein synthesis kits, the technology is expanding both in the range of feasible proteins and in the ways that proteins can be labeled and modified. This volume follows "Cell-Free Translation Systems", edited by Professor Alexander S. Spirin in 2002. Since then, an impressive collection of new work has emerged that demonstrates a substantial expansion of capability. In this volume, we show that proteins now can be efficiently produced using PCR products as DNA templates and that even membrane proteins and proteins with multiple disulfide proteins are obtained at high yields. Many additional advances are also presented. It is an exciting time for protein synthesis technology.
Author | : Alexander S. Spirin |
Publisher | : John Wiley & Sons |
Total Pages | : 272 |
Release | : 2007-12-03 |
Genre | : Science |
ISBN | : 9783527316496 |
Download Cell-free Protein Synthesis Book in PDF, ePub and Kindle
With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.
Author | : Jian Li |
Publisher | : Frontiers Media SA |
Total Pages | : 205 |
Release | : 2022-01-13 |
Genre | : Science |
ISBN | : 2889740803 |
Download Cell-Free Synthetic Biology Book in PDF, ePub and Kindle
Author | : W. Antoni Kudlicki |
Publisher | : CRC Press |
Total Pages | : 242 |
Release | : 2007-11-27 |
Genre | : Science |
ISBN | : 1498713297 |
Download Cell-Free Protein Expression Book in PDF, ePub and Kindle
Following its inception in the 1950s, cell-free protein synthesis made a tremendous impact on the basic life sciences. The use of cell-free systems was key to understanding molecular mechanisms underlying one of the most complicated processes found in nature: protein translation. Since this time, aggressive cutting-edge research and stiff commerica
Author | : Alexander S. Spirin |
Publisher | : John Wiley & Sons |
Total Pages | : 272 |
Release | : 2014-08-15 |
Genre | : Science |
ISBN | : 3527691502 |
Download Cell-free Protein Synthesis Book in PDF, ePub and Kindle
With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.
Author | : Ron Milo |
Publisher | : Garland Science |
Total Pages | : 400 |
Release | : 2015-12-07 |
Genre | : Science |
ISBN | : 1317230698 |
Download Cell Biology by the Numbers Book in PDF, ePub and Kindle
A Top 25 CHOICE 2016 Title, and recipient of the CHOICE Outstanding Academic Title (OAT) Award. How much energy is released in ATP hydrolysis? How many mRNAs are in a cell? How genetically similar are two random people? What is faster, transcription or translation?Cell Biology by the Numbers explores these questions and dozens of others provid
Author | : Bruce Alberts |
Publisher | : |
Total Pages | : 0 |
Release | : 2002 |
Genre | : Cytology |
ISBN | : 9780815332183 |
Download Molecular Biology of The Cell Book in PDF, ePub and Kindle
Author | : Christina Smolke |
Publisher | : John Wiley & Sons |
Total Pages | : 532 |
Release | : 2018-02-28 |
Genre | : Science |
ISBN | : 3527688099 |
Download Synthetic Biology Book in PDF, ePub and Kindle
A review of the interdisciplinary field of synthetic biology, from genome design to spatial engineering. Written by an international panel of experts, Synthetic Biology draws from various areas of research in biology and engineering and explores the current applications to provide an authoritative overview of this burgeoning field. The text reviews the synthesis of DNA and genome engineering and offers a discussion of the parts and devices that control protein expression and activity. The authors include information on the devices that support spatial engineering, RNA switches and explore the early applications of synthetic biology in protein synthesis, generation of pathway libraries, and immunotherapy. Filled with the most recent research, compelling discussions, and unique perspectives, Synthetic Biology offers an important resource for understanding how this new branch of science can improve on applications for industry or biological research.