Cell Free Gene Expression PDF Download
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Author | : Ashty S. Karim |
Publisher | : |
Total Pages | : 435 |
Release | : 2022 |
Genre | : Biology |
ISBN | : 9781071619988 |
Download Cell-Free Gene Expression Book in PDF, ePub and Kindle
This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell‐Free Gene Expression: Methods and Protocols serves as an ideal guide for researchers seeking technical methods to current aspects of CFE and related applications.
Author | : James R. Swartz |
Publisher | : Springer Science & Business Media |
Total Pages | : 213 |
Release | : 2012-12-06 |
Genre | : Science |
ISBN | : 3642593372 |
Download Cell-Free Protein Expression Book in PDF, ePub and Kindle
Cell-free protein synthesis is coming of age! Motivated by an escalating need for efficient protein synthesis and empowered by readily accessible cell-free protein synthesis kits, the technology is expanding both in the range of feasible proteins and in the ways that proteins can be labeled and modified. This volume follows "Cell-Free Translation Systems", edited by Professor Alexander S. Spirin in 2002. Since then, an impressive collection of new work has emerged that demonstrates a substantial expansion of capability. In this volume, we show that proteins now can be efficiently produced using PCR products as DNA templates and that even membrane proteins and proteins with multiple disulfide proteins are obtained at high yields. Many additional advances are also presented. It is an exciting time for protein synthesis technology.
Author | : Jian Li |
Publisher | : Frontiers Media SA |
Total Pages | : 205 |
Release | : 2022-01-13 |
Genre | : Science |
ISBN | : 2889740803 |
Download Cell-Free Synthetic Biology Book in PDF, ePub and Kindle
Author | : W. Antoni Kudlicki |
Publisher | : CRC Press |
Total Pages | : 242 |
Release | : 2007-11-27 |
Genre | : Science |
ISBN | : 1498713297 |
Download Cell-Free Protein Expression Book in PDF, ePub and Kindle
Following its inception in the 1950s, cell-free protein synthesis made a tremendous impact on the basic life sciences. The use of cell-free systems was key to understanding molecular mechanisms underlying one of the most complicated processes found in nature: protein translation. Since this time, aggressive cutting-edge research and stiff commerica
Author | : A.S. Spirin |
Publisher | : Springer Science & Business Media |
Total Pages | : 254 |
Release | : 2012-12-06 |
Genre | : Science |
ISBN | : 3642593798 |
Download Cell-Free Translation Systems Book in PDF, ePub and Kindle
This is a unique book that describes the most recent achievements in the methodology of protein biosynthesis under cell-free conditions. Various versions of cell-free protein-synthesizing systems and their applications to production of individual proteins on a preparative scale are reviewed. The most recent, advanced methodologies, such as continuous-exchange and continuous-flow cell-free systems and novel effecting batch-format cell-free procedures, are considered. Special attention is drawn to the possibilities of structural (NMR; X-ray) analysis of various gene expression products with the use of a new generation of cell-free systems.
Author | : Bruce Alberts |
Publisher | : |
Total Pages | : 0 |
Release | : 2002 |
Genre | : Cytology |
ISBN | : 9780815332183 |
Download Molecular Biology of The Cell Book in PDF, ePub and Kindle
Author | : Alexander S. Spirin |
Publisher | : John Wiley & Sons |
Total Pages | : 272 |
Release | : 2014-08-15 |
Genre | : Science |
ISBN | : 3527691502 |
Download Cell-free Protein Synthesis Book in PDF, ePub and Kindle
With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.
Author | : Christoph Wittmann |
Publisher | : John Wiley & Sons |
Total Pages | : 789 |
Release | : 2017-04-10 |
Genre | : Science |
ISBN | : 352734179X |
Download Industrial Biotechnology Book in PDF, ePub and Kindle
The latest volume in the Advanced Biotechnology series provides an overview of the main production hosts and platform organisms used today as well as promising future cell factories in a two volume book. Alongside describing tools for genetic and metabolic engineering for strain improvement, the authors also impart topical information on computational tools, safety aspects and industrial-scale production. Following an introduction to general concepts, historical developments and future technologies, the text goes on to cover multi-purpose bacterial cell factories, including those organisms that exploit anaerobic biosynthetic power. Further chapters deal with microbes used for the production of high-value natural compounds and those obtained from alternative raw material sources, concluding with eukaryotic workhorses.
Author | : Ashty S. Karim |
Publisher | : Humana |
Total Pages | : 435 |
Release | : 2022-01-06 |
Genre | : Science |
ISBN | : 9781071619971 |
Download Cell-Free Gene Expression Book in PDF, ePub and Kindle
This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell‐Free Gene Expression: Methods and Protocols serves as an ideal guide for researchers seeking technical methods to current aspects of CFE and related applications.
Author | : Paulina Balbas |
Publisher | : Springer Science & Business Media |
Total Pages | : 505 |
Release | : 2008-02-04 |
Genre | : Science |
ISBN | : 1592597742 |
Download Recombinant Gene Expression Book in PDF, ePub and Kindle
Since newly created beings are often perceived as either wholly good or bad, the genetic alteration of living cells impacts directly on a symbolic meaning deeply imbedded in every culture. During the earlier years of gene expression research, te- nological applications were confined mainly to academic and industrial laboratories, and were perceived as highly beneficial since molecules that were previously unable to be separated or synthesized became accessible as therapeutic agents. Such were the success stories of hormones, antibodies, and vaccines produced in the bacterium Escherichia coli. Originally this bacterium gained fame among humans for being an unwanted host in the intestine, or worse yet, for being occasionally dangerous and pathogenic. H- ever, it was easily identified in contaminated waters during the 19th century, thus becoming a clear indicator of water pollution by human feces. Tamed, cultivated, and easily maintained in laboratories, its fast growth rate and metabolic capacity to adjust to changing environments fascinated the minds of scientists who studied and modeled such complex phenomena as growth, evolution, genetic exchange, infection, survival, adaptation, and further on—gene expression. Although at the lower end of the complexity scale, this microbe became a very successful model system and a key player in the fantastic revolution kindled by the birth of recombinant DNA technology.