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Biosecurity: A Systems Perspective provides an overview of biosecurity as a system of related components, actors, and risks. This book—directed to the biosecurity practitioner, generalist scientist, and student—introduces overall features of the biosecurity system while walking the reader through the most up-to-date research on each step of the continuum (i.e. pre-border, border, and post-border activities). This book, which explicitly incorporates economic and social dimensions as well as varied decision-making contexts, paves the way for a more systemic approach to biosecurity risk management. The work spans statistics, ecology, mathematics, economics, veterinary science, human medicine, and sociology, involving collaborators across government, academia, and the private sector. This book uses a broad definition of biosecurity, rather than solely focusing on plant health, animal health, security, or one step of the biosecurity system (e.g. surveillance). As such, this book is a one-stop shop for readers interested in all aspects of biosecurity. The content and language are accessible to a wide range of audiences, including generalist scientists, biosecurity practitioners, and graduate students. More complex content is introduced in standalone boxes or chapters. The book follows a clear, simple structure within and among chapters (i.e. following the structure of the biosecurity system), making it a preferred option for graduate students. This book introduces novel cross-cutting themes, such as the importance of economic efficiency and institutional and social factors, going beyond the traditional science-based approach to biosecurity. Written in partnership with government agency practitioners, this book uses on-the-ground case studies to demonstrate how biosecurity principles are applied in practice. The book addresses challenges common to regulators in general, namely efficient regulation in uncertain and rapidly changing environments with multiple stakeholders who have—at times—conflicting priorities.