Biomimetic Model Membranes To Study Protein Membrane Interactions And Their Role In Alzheimers Disease PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Biomimetic Model Membranes To Study Protein Membrane Interactions And Their Role In Alzheimers Disease PDF full book. Access full book title Biomimetic Model Membranes To Study Protein Membrane Interactions And Their Role In Alzheimers Disease.
| Author | : |
| Publisher | : |
| Total Pages | : 33 |
| Release | : 2015 |
| Genre | : Alzheimer's disease |
| ISBN | : |
Download Biomimetic Model Membranes to Study Protein-membrane Interactions and Their Role in Alzheimer's Disease Book in PDF, ePub and Kindle
Lipid-coated AuNPs are prepared as cellular membrane mimics to study the protein- membrane interactions that play a role in neurodegeneration. PC and PC/Chol (1:1) lipid- coated AuNPs are used to monitor [amyloid beta] oligomer and monomer cellular membrane interactions. We used fluorescence anisotropy and TAMRA conjugated [amyloid beta]1-42 (TAMRA-[amyloid beta]1-42) to determine [amyloid beta] binding to lipid-coated AuNPs. An increase in the fluorescence anisotropy (r) of TAMRA-[amyloid beta]1-42 in the presence of the lipid-coated AuNPs indicate that the [amyloid beta] monomers bind to the membrane surface. Based on the change in r the binding affinity of [amyloid beta] to the AuNPs at pH 6.5 is higher than at pH 8 and was greater for Au-SO-PC-HT compared Au-SO-PC-Chol- HT. Dynamic light scattering (DLS) confirmed that [amyloid beta]1-42 monomers as well as oligomers bind to the lipid bilayers of the AuNPs as is evident by an increase in the hydrodynamic diameter (HD) of the lipid-coated AuNPs. When incubated with [amyloid beta] monomers, DLS show the greatest increase in the HD of Au-SO-PC-HT verses the Au-SO-PC-Chol-HT. When incubated with [amyloid beta] oligomers, DLS a greater increase in HD is observed at pH 6.5 compared to 8, demonstrating that [amyloid beta] binding is pH dependent. To investigate if [amyloid beta] binding to lipid-coated AuNPs cause significant disruption in membrane integrity cyanide etching studies were performed. UV-vis showed no shift in the surface plasmon resonance or change in optical density of lipid-coated AuNPs for either membrane type at pH 6.5 or 8.0 demonstrating that [amyloid beta] binding to the AuNP membranes does not lead to significant membrane disruption.
| Author | : Sidney A. Simon |
| Publisher | : Academic Press |
| Total Pages | : 606 |
| Release | : 2002-11-13 |
| Genre | : Science |
| ISBN | : 0080925855 |
Download Peptide-Lipid Interactions Book in PDF, ePub and Kindle
This volume contains a comprehensive overview of peptide-lipid interactions by leading researchers. The first part covers theoretical concepts, experimental considerations, and thermodynamics. The second part presents new results obtained through site-directed EPR, electron microscopy, NMR, isothermal calorimetry, and fluorescence quenching. The final part covers problems of biological interest, including signal transduction, membrane transport, fusion, and adhesion. Key Features * world-renowned experts * state-of-the-art experimental methods * monolayers, bilayers, biological membranes * theoretical aspects and computer simulations * rafts * synaptic transmission * membrane fusion * signal transduction
| Author | : J. Katsaras |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 304 |
| Release | : 2013-06-29 |
| Genre | : Science |
| ISBN | : 366204496X |
Download Lipid Bilayers Book in PDF, ePub and Kindle
Provides the reader with an up to date insight of the current state of the art in the field of lipid bilayer research and the important insights derived for the understanding of the complex and varied behaviour of biological membranes and its function.
| Author | : C.G. Gebelein |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 297 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 1461306574 |
Download Biomimetic Polymers Book in PDF, ePub and Kindle
The term biomimetic is comparatively new on the chemical scene, but the concept has been utilized by chemists for many years. Furthermore, the basic idea of making a synthetic material that can imitate the func tions of natural materials probably could be traced back into antiquity. From the dawn of creation, people have probably attempted to duplicate or modify the activities of the natural world. (One can even find allusions to these attempts in the Bible; e. g. , Genesis 30. ) The term "mimetic" means to imitate or mimic. The word "mimic" means to copy closely, or to imitate accurately. Biomimetic, which has not yet entered most dictionaries, means to imitate or mimic some specific bio logical function. Usually, the objective of biomimetics is to form some useful material without the need of utilizing living systems. In a simi lar manner, the term biomimetic polymers means creating synthetic poly mers which imitate the activity of natural bioactive polymers. This is a major advance in polymer chemistry because the natural bioactive polymers are the basis of life itself. Thus, biomimetic polymers imitate the life process in many ways. This present volume delineates some of the recent progress being made in this vast field of biomimetic polymers. Chemists have been making biomimetic polymers for more than fifty years, although this term wasn't used in the early investigations.
| Author | : Einar M. Sigurdsson |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 390 |
| Release | : 2008-02-02 |
| Genre | : Science |
| ISBN | : 1592598749 |
Download Amyloid Proteins Book in PDF, ePub and Kindle
A proven collection of readily reproducible techniques for studying amyloid proteins and their involvement in the etiology, pathogenesis, diagnosis, and therapy of amyloid diseases. The contributors provide methods for the preparation of amyloid and its precursors (oligomers and protofibrils), in vitro assays and analytical techniques for their study, and cell culture models and assays for the production of amyloid proteins. Additional chapters present readily reproducible techniques for amyloid extraction from tissue, its detection in vitro and in vivo, as well as nontransgenic methods for developing amyloid mouse models. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
| Author | : Detlev Boison |
| Publisher | : Frontiers Media SA |
| Total Pages | : 256 |
| Release | : 2022-11-21 |
| Genre | : Science |
| ISBN | : 2832506658 |
Download Insights in Brain Disease Mechanisms: 2021 Book in PDF, ePub and Kindle
| Author | : Roy Bicknell |
| Publisher | : Cambridge University Press |
| Total Pages | : 156 |
| Release | : 1996-09-28 |
| Genre | : Science |
| ISBN | : 9780521559904 |
Download Endothelial Cell Culture Book in PDF, ePub and Kindle
The aim of the Handbooks in Practical Animal Cell Biology is to provide practical workbooks for those involved in primary cell culture. Each volume addresses a different cell lineage, and contains an introductory section followed by individual chapters on the culture of specific differentiated cell types. The authors of each chapter are leading researchers in their fields and use their first-hand experience to present reliable techniques in a clear and thorough manner. Endothelial Cell Culture contains chapters on endothelial cells derived from 1) lung, 2) bone marrow, 3) brain, 4) mammary glands, 5) skin, 6) adipose tissue, 7) female reproductive system, and 8) synovium.
| Author | : Véronique Arluison |
| Publisher | : Springer Nature |
| Total Pages | : 336 |
| Release | : 2022-08-11 |
| Genre | : Science |
| ISBN | : 1071625292 |
Download Bacterial Amyloids Book in PDF, ePub and Kindle
This detailed volume introduces different methods to assess the structure and folding of bacterial amyloids and analyze their functions, either in vitro or in vivo. Despite their initial association with neurodegenerative diseases, there is now increasing evidence of alternative roles for amyloids, with beneficial aspects for cells. In particular, prokaryotes use amyloids as functional assemblies, where they are key players in the cell's physiology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Bacterial Amyloids: Methods and Protocols is an ideal guide for experts and novices alike to further explore these crucial protein assemblies.
| Author | : E. Edward Bittar |
| Publisher | : JAI Press(NY) |
| Total Pages | : 0 |
| Release | : 1997 |
| Genre | : Cell-mediated cytotoxicity |
| ISBN | : 9780762301416 |
Download Mechanisms of Cell Toxicity Book in PDF, ePub and Kindle
Cellular toxicology has entered a new era. No longer are we concerned only with necrotic cell death produced by severe, acute insult (often to multiple intracellular targets) leading to disruption of the cell membrane. New advances in molecular and cellular biology are allowing the dissection of mechanisms of cell death involving more subtle targets within the cell. Toxicology has been very important, not only in understanding the mechanisms, nature, and severity of toxicity and thereby helping in risk assessment, but toxicology has also played a very important role in helping to understand basic biological processes. Historically this has perhaps been most evident in the use of toxic agents to interfere with specific reactions in the body and hence help to dissect out the mechanisms of metabolic processes. For example, the use of chemical inhibitors was very important in understanding the process of oxidative phosphorylation, or the tricarboxylic acid cycle. More recent examples are seen herein where toxicology interfaces with, for example structural biology in the study of the cytoskeletal components and their interactions. Indirectly, an understanding of the mechanisms of endogenous protective systems also improves knowledge of basic cell biology. Toxic insult and manipulation of cell signalling and control mechanisms in cell growth and differentation also highlight how important the discipline of cell toxicity has been and will continue to be a major contributor to our understanding of basic issues in the biological and biomedical sciences. This book offers selected reviews of some of the principal molecular mechanisms of cell toxicity.
| Author | : R. Lipowsky |
| Publisher | : Elsevier |
| Total Pages | : 537 |
| Release | : 1995-06-15 |
| Genre | : Technology & Engineering |
| ISBN | : 0080541917 |
Download Structure and Dynamics of Membranes Book in PDF, ePub and Kindle
The first volume of the Handbook deals with the amazing world of biomembranes and lipid bilayers. Part A describes all aspects related to the morphology of these membranes, beginning with the complex architecture of biomembranes, continues with a description of the bizarre morphology of lipid bilayers and concludes with technological applications of these membranes. The first two chapters deal with biomembranes, providing an introduction to the membranes of eucaryotes and a description of the evolution of membranes. The following chapters are concerned with different aspects of lipids including the physical properties of model membranes composed of lipid-protein mixtures, lateralphase separation of lipids and proteins and measurement of lipid-protein bilayer diffusion. Other chapters deal with the flexibility of fluid bilayers, the closure of bilayers into vesicles which attain a large variety of different shapes, and applications of lipid vesicles and liposomes. Part B covers membrane adhesion, membrane fusion and the interaction of biomembranes withpolymer networks such as the cytoskeleton. The first two chapters of this part discuss the generic interactions of membranes from the conceptual point of view. The following two chapters summarize the experimental work on two different bilayer systems. The next chapter deals with the process ofcontact formation, focal bounding and macroscopic contacts between cells. The cytoskeleton within eucaryotic cells consists of a network of relatively stiff filaments of which three different types of filaments have been identified. As explained in the next chapter much has been recently learned aboutthe interaction of these filaments with the cell membrane. The final two chapters deal with membrane fusion.
