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Basic Techniques in Biochemistry, Microbiology and Molecular Biology

Basic Techniques in Biochemistry, Microbiology and Molecular Biology
Author: Aakanchha Jain
Publisher:
Total Pages: 282
Release: 2020
Genre: Biochemistry
ISBN: 9781493998616

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This book presents key methodologies, tools and databases for biochemistry, microbiology and molecular biology in simple and straightforward language. Covering all aspects related to experimental principles and procedures, the protocols included here are brief and clearly defined, and include essential precautions to be taken while conducting experiments. The book is divided into two major sections: one on constructing, working with, and standard operating procedures for laboratory instruments; and one on practical procedures used in molecular biology, microbiology and biochemical analysis experiments, which are described in full. Each chapter describes both the basic theory and relevant practical details for a given experiment, and helps readers recognize both the experiment's potential and limitations. Intended as an intensive introduction to the various tools used in molecular biology, the book covers all basic methods and equipment, including cloning, PCR, spectrophotometers, ELISA readers, sonicators, etc. As such, it offers a valuable asset for final year undergraduate (especially project) students, graduate research students, research scientists and technicians who wish to understand and employ new techniques in the field of biotechnology.


Basic Techniques in Biochemistry, Microbiology and Molecular Biology

Basic Techniques in Biochemistry, Microbiology and Molecular Biology
Author: Aakanchha Jain
Publisher: Humana
Total Pages: 282
Release: 2021-03-14
Genre: Medical
ISBN: 9781493998630

Download Basic Techniques in Biochemistry, Microbiology and Molecular Biology Book in PDF, ePub and Kindle

This book presents key methodologies, tools and databases for biochemistry, microbiology and molecular biology in simple and straightforward language. Covering all aspects related to experimental principles and procedures, the protocols included here are brief and clearly defined, and include essential precautions to be taken while conducting experiments. The book is divided into two major sections: one on constructing, working with, and standard operating procedures for laboratory instruments; and one on practical procedures used in molecular biology, microbiology and biochemical analysis experiments, which are described in full. Each chapter describes both the basic theory and relevant practical details for a given experiment, and helps readers recognize both the experiment’s potential and limitations. Intended as an intensive introduction to the various tools used in molecular biology, the book covers all basic methods and equipment, including cloning, PCR, spectrophotometers, ELISA readers, sonicators, etc. As such, it offers a valuable asset for final year undergraduate (especially project) students, graduate research students, research scientists and technicians who wish to understand and employ new techniques in the field of biotechnology.


Basic Techniques in Biochemistry and Molecular Biology

Basic Techniques in Biochemistry and Molecular Biology
Author: R. K. Sharma
Publisher: I. K. International Pvt Ltd
Total Pages: 278
Release: 2013-12-30
Genre: Biochemistry
ISBN: 819074626X

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Fundamentals of biochemistry and molecular biology is an important component of all disciplines of Biology. In the era of multidisciplinary approach, the basic techniques in Biochemistry and Molecular Biology are much needed by the students of Botany, Zoology, Microbiology, Biotechnology, Fisheries, Veterinary, Pharmacology, Physiology, Medicine, Genetics, Agriculture and allied subjects both at undergraduate and postgraduate levels. This book includes 15 chapters covering more than 135 experimental protocols. It discussed all the relevant topics like pH and buffers, spectrophotometry, chromatography, carbohydrates, lipids, proteins, electrophoresis, enzyme immunology, vitamins and pigments, metabolites and molecular biology. It includes a wide range of experiments from preparation of culture media to PCR, Southern and Western blotting. All the experiments have been meticulously designed and special care has been taken to the safety in laboratory and precautions are given wheresoever required.


Principles and Techniques of Biochemistry and Molecular Biology

Principles and Techniques of Biochemistry and Molecular Biology
Author: Keith Wilson
Publisher: Cambridge University Press
Total Pages: 769
Release: 2010-03-04
Genre: Medical
ISBN: 0521516358

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Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.


Analytical Techniques in Biochemistry and Molecular Biology

Analytical Techniques in Biochemistry and Molecular Biology
Author: Rajan Katoch
Publisher: Springer Science & Business Media
Total Pages: 449
Release: 2011-07-19
Genre: Science
ISBN: 1441997857

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Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.


Basic Techniques in Molecular Biology

Basic Techniques in Molecular Biology
Author: Stefan Surzycki
Publisher: Springer Science & Business Media
Total Pages: 442
Release: 2012-12-06
Genre: Science
ISBN: 3642569684

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This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.


Advances in Protein Molecular and Structural Biology Methods

Advances in Protein Molecular and Structural Biology Methods
Author: Timir Tripathi
Publisher: Academic Press
Total Pages: 716
Release: 2022-01-14
Genre: Science
ISBN: 0323902650

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Advances in Protein Molecular and Structural Biology Methods offers a complete overview of the latest tools and methods applicable to the study of proteins at the molecular and structural level. The book begins with sections exploring tools to optimize recombinant protein expression and biophysical techniques such as fluorescence spectroscopy, NMR, mass spectrometry, cryo-electron microscopy, and X-ray crystallography. It then moves towards computational approaches, considering structural bioinformatics, molecular dynamics simulations, and deep machine learning technologies. The book also covers methods applied to intrinsically disordered proteins (IDPs)followed by chapters on protein interaction networks, protein function, and protein design and engineering. It provides researchers with an extensive toolkit of methods and techniques to draw from when conducting their own experimental work, taking them from foundational concepts to practical application. Presents a thorough overview of the latest and emerging methods and technologies for protein study Explores biophysical techniques, including nuclear magnetic resonance, X-ray crystallography, and cryo-electron microscopy Includes computational and machine learning methods Features a section dedicated to tools and techniques specific to studying intrinsically disordered proteins


Molecular Biology Techniques

Molecular Biology Techniques
Author: Heather Miller
Publisher: Academic Press
Total Pages: 232
Release: 2011-10-18
Genre: Science
ISBN: 0123855454

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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions


MICROBIOLOGICAL TECHNIQUES

MICROBIOLOGICAL TECHNIQUES
Author: N. Murugalatha, Lali Growther, J. Vimalin Hena, N. Hema Shenpagam, R. Anitha, D. Kanchana Devi, G. Rajalakshmi
Publisher: MJP Publisher
Total Pages: 456
Release:
Genre: Medical
ISBN:

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CONTENTS :- 1. Introduction to Microbiology, 2. Tools of Microbiology, 3. Fundamentals of Microbiology, 4. Microbial Physiology, 5. Industrial Microbiology, 6. Environmental Microbiology, 7. Food Microbiology, 8. Genetics, 9. Immunology, 10. Medical Microbiology, 11. Biochemical Methodology, 12. Virology. PREFACE :- Microbiological Techniques is designed for the students, to explore the world of microorganisms and how the process of scientific discovery is carried out, with an ease. The study of microbiology is dynamic because of the ubiquitous nature of the microbes and the variability inherent in every living organism. The broad nature of the subject and diversity of topics from the fundamentals to its unique fields can make the way of presentation a little difficult; but it is also a part of what makes microbiology an interesting and challenging subject. The book primarily focuses on the basic microbiological techniques with applications for undergraduate and postgraduate students in diverse area of biological techniques. This book is the outcome of nearly a decade of teaching and research experience. The manual comprises twelve parts in which exercises in first three parts provide sequential developments of fundamental techniques. The remaining exercises are as independent as possible to allow the instructor to select the desirable sequence. Exercises are pursued in a normal scale providing maximum details so that one can perform the experiment independently and safely. The style and simplicity of expression have been our twin objectives. All exercises have been thoroughly tested in our laboratory by our students with wide variety of real talents and enthusiasm.


Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology

Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology
Author: Andreas Hofmann
Publisher: Cambridge University Press
Total Pages: 962
Release: 2018-04-19
Genre: Science
ISBN: 1108365256

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Bringing this best-selling textbook right up to date, the new edition uniquely integrates the theories and methods that drive the fields of biology, biotechnology and medicine, comprehensively covering both the techniques students will encounter in lab classes and those that underpin current key advances and discoveries. The contents have been updated to include both traditional and cutting-edge techniques most commonly used in current life science research. Emphasis is placed on understanding the theory behind the techniques, as well as analysis of the resulting data. New chapters cover proteomics, genomics, metabolomics, bioinformatics, as well as data analysis and visualisation. Using accessible language to describe concepts and methods, and with a wealth of new in-text worked examples to challenge students' understanding, this textbook provides an essential guide to the key techniques used in current bioscience research.