Aqueous Two Phase Partitioning PDF Download
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| Author | : Harry Walter |
| Publisher | : Elsevier |
| Total Pages | : 731 |
| Release | : 2012-12-02 |
| Genre | : Science |
| ISBN | : 0323142915 |
Download Partitioning In Aqueous Two – Phase System Book in PDF, ePub and Kindle
Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.
| Author | : Rajni Hatti-Kaul |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 442 |
| Release | : 2008-02-05 |
| Genre | : Science |
| ISBN | : 1592590284 |
Download Aqueous Two-Phase Systems Book in PDF, ePub and Kindle
A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations. Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by P.-Å. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for large-scale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of two-phase systems has for a long time been confined to selected labora- ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ two-phase partitioning techniques in both basic and applied research.
| Author | : Boris Y. Zaslavsky |
| Publisher | : CRC Press |
| Total Pages | : 720 |
| Release | : 1994-11-15 |
| Genre | : Science |
| ISBN | : 9780824794613 |
Download Aqueous Two-Phase Partitioning Book in PDF, ePub and Kindle
Covers the fundamental principles of solute partitioning in aqueous two-phase systems, explains their important practical features, and furnishes methods of characterization. The information provided by the partition behaviour of a solute in an aqueous two-phase system is examined.
| Author | : Marco Rito-Palomares |
| Publisher | : Springer |
| Total Pages | : 196 |
| Release | : 2017-07-18 |
| Genre | : Technology & Engineering |
| ISBN | : 3319593099 |
Download Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products Book in PDF, ePub and Kindle
This comprehensive and unique text presents a full overview of downstream processing useful for those new to the concept as well as professionals with experience in the area. The history and theoretical principles of Aqueous Two-Phase Systems (ATPS) are covered in depth. Information on ATPS characterization and application is included, and ATPS equilibria and system parameters that have significant effect on partition behavior are studied. Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products addresses specific applications of ATPS for the recovery and partial purification of high molecular weight compounds such as proteins, nucleic acids and polysaccharides, particulate bioproducts such as cells and organelles and low molecular weight compounds. Non-conventional strategies involving ATPS such as affinity systems, continuous liquid-liquid fractionation stages and the recovery from plant extracts are presented. Economic analysis of the application of ATPS in comparison to other fractionation techniques, particularly liquid chromatography, is considered, as are opportunity and current trends in the ATPS research area. Each chapter utilizes the contributors' experimental expertise in traditional and non-conventional ATPS strategies, as well as analysis of areas of opportunity and perspectives on the development and future applications of ATPS in both the lab and larger scale operations. The result is a thorough and singular overview of ATPS which has not been matched by any other text on the market.
| Author | : Roza Maria Kamp |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 311 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 3642592198 |
Download Protein Structure Analysis Book in PDF, ePub and Kindle
"Protein Structure Analysis - Preparation and Characterization" is a compilation of practical approaches to the structural analysis of proteins and peptides. Here, about 20 authors describe and comment on techniques for sensitive protein purification and analysis. These methods are used worldwide in biochemical and biotechnical research currently being carried out in pharmaceu tical and biomedical laboratories or protein sequencing facilities. The chapters have been written by scientists with extensive ex perience in these fields, and the practical parts are well documen ted so that the reader should be able to easily reproduce the described techniques. The methods compiled in this book were demonstrated in student courses and in the EMBO Practical Course on "Microsequence Analysis of Proteins" held in Berlin September 10-15, 1995. The topics also derived from a FEBS Workshop, held in Halkidiki, Thessaloniki, Greece, in April, 1995. Most of the authors participated in these courses as lecturers and tutors and made these courses extremely lively and successful. Since polypeptides greatly vary depending on their specific structure and function, strategies for their structural analysis must for the most part be adapted to each individual protein. Therefore, advantages and limitations of the experimen tal approaches are discussed here critically, so that the reader becomes familiar with problems that might be encountered.
| Author | : Andrzej Gorak |
| Publisher | : Royal Society of Chemistry |
| Total Pages | : 544 |
| Release | : 2018-06-18 |
| Genre | : Science |
| ISBN | : 1788010329 |
Download Intensification of Biobased Processes Book in PDF, ePub and Kindle
The first book dedicated entirely to this area, Intensification of Biobased Processes provides a comprehensive overview of modern process intensification technologies used in bioprocessing.
| Author | : Rajni Hatti-Kaul |
| Publisher | : |
| Total Pages | : |
| Release | : 2000 |
| Genre | : |
| ISBN | : |
Download Aqueous Two-Phase Systems Book in PDF, ePub and Kindle
Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods to cutting-edge techniques with potential biotechnological applications. Among the methods detailed are those for ATPS preparation and characterization, for partitioning applied to soluble molecules and particulates (including whole cells, membranes, and organelles), and for the isolation and purification of proteins - including a glimpse of large-scale handling of two-phase separations. Practical and informative, with its detailed guidelines allowing researchers to adapt specific systems to their own separation needs, Aqueous Two-Phase Systems: Methods and Protocols demonstrates the scope and utility of two-phase aqueous systems in both basic and applied research.
| Author | : Mara G. Freire |
| Publisher | : Springer |
| Total Pages | : 322 |
| Release | : 2016-10-05 |
| Genre | : Technology & Engineering |
| ISBN | : 3662528754 |
Download Ionic-Liquid-Based Aqueous Biphasic Systems Book in PDF, ePub and Kindle
This book offers comprehensive information on the fundamentals and applications of ionic-liquid-based aqueous biphasic systems, which have predominantly (and successfully) been employed as alternative platforms for the extraction, separation and purification of diverse high-value products. The book consists of an initial introduction providing a brief overview, from fundamentals to applications, followed by nine chapters addressing the respective phase diagrams (interpretation and characterization) and remarkable examples of their applications. It also includes two final chapters focusing on recent developments in the search for more environmentally-benign and biocompatible ionic-liquid-based aqueous biphasic systems, and on the progress made to date concerning the recovery, recycling and reuse of the phase-forming components, the goal being the development of cost-effective and sustainable processes. The book offers an interesting and useful guide for a broad readership in the fields of green chemistry, biotechnology, chemical engineering, and biochemistry, among others. Mara G. Freire is a Coordinator Researcher at CICECO - Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Portugal.
| Author | : Munishwar Nath Gupta |
| Publisher | : Elsevier |
| Total Pages | : 346 |
| Release | : 2021-08-11 |
| Genre | : Science |
| ISBN | : 0323859038 |
Download Three Phase Partitioning Book in PDF, ePub and Kindle
Three Phase Partitioning: Applications in Separation and Purification of Biological Molecules and Natural Products presents applications in diverse areas of both chemical technology and biotechnology. This book serves as a single resource for learning about both the economical, facile and scalable processes, along with their potential for applications in the separation and purification of materials and compounds across the entire spectra of chemical and biological nature. The book begins by explaining the origins and fundamentals of TPP and continues with chapters on related applications, ranging from the purification of parasite recombinant proteases to oil extraction from oilseeds and oleaginous microbes, and more. Written by researchers who have been pioneers in developing and utilizing three phase partitioning Focuses on applications, with chapters detailing relevance to a wide variety of areas and numerous practical examples Designed to give laboratory workers the information needed to undertake the challenge of designing successful three-phase partitioning protocols
| Author | : Harry Walter |
| Publisher | : Elsevier |
| Total Pages | : 768 |
| Release | : 1994-04-18 |
| Genre | : Nature |
| ISBN | : 9780121821296 |
Download Aqueous Two-Phase Systems Book in PDF, ePub and Kindle
General methodology and apparatus: phase diagrams, preparation and analysis of two-phase systems, partioning and affinity partitioning of macromolecules: Proteins, nucleic acids, studies on protein interactionsmolecular structure, charge, hydrophobicity, and conformational chan ges, partitioning and affinity partitioning of particulates,organellesseparation and subfractionation, menbrane: separation and subfractionation, membrane domain analysis, aqueous phase separation in biologicalsystems, aqueous two-phase systems in large-scale process biotechnolo gy, proteins; downstream processing, design of proteins for enhanced extraction, other applications of aqueous phases in biotechnology. Enzymology.
