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| Author | : Achirapa Bandhaya |
| Publisher | : |
| Total Pages | : |
| Release | : 2013 |
| Genre | : |
| ISBN | : |
| Author | : Gulab Singh Yadav |
| Publisher | : Springer Nature |
| Total Pages | : 186 |
| Release | : 2019-11-11 |
| Genre | : Science |
| ISBN | : 9811388369 |
The book discusses the basics of aptamers and the advent of aptamer-based technology in recent times. The book covers the diverse applications of aptamers, such as in detection of animal and plant pathogens, disease diagnosis and therapeutics, environmental contamination detection etc. Besides these applications, the book also describes the use of these synthetic or modified DNA, as drug delivery vehicles. The different chapters describe how the binding capacity and specificity of aptamers can be exploited in various ways. The book also discusses how these attributes of aptamers can outdo the antibody technology in biomedical and diagnostic solutions. This crisp and concise book gives the readers an insight into the most recent biotechnological applications of aptamers.
| Author | : Rakesh N. Veedu |
| Publisher | : CRC Press |
| Total Pages | : 386 |
| Release | : 2017-03-03 |
| Genre | : Medical |
| ISBN | : 9814669849 |
Aptamers, often termed as ‘chemical antibodies,’ are an emerging class of synthetic ligands for efficient target-specific molecular recognition. The objective of this book is to highlight recent advances and potential of aptamers in various disease conditions. . This book focuses on the applications of aptamers in targeted nanotherapy, detection, and in molecular imaging in various disease conditions such as cancer, neurological diseases and infectious diseases.
| Author | : Weihong Tan |
| Publisher | : Springer |
| Total Pages | : 352 |
| Release | : 2015-03-02 |
| Genre | : Science |
| ISBN | : 3662462265 |
This edited volume describes cell-SELEX as the fundamental tool used to generate aptamer molecules for a wide range of applications in molecular medicine, bioanalysis and chemical biology. Easily integrated into the natural heterogeneous cell matrix, aptamers can be effectively used in theranostics, bioanalysis, environment detection and biomedical studies. The book gathers reviews that reflect the latest advances in the field of aptamers and consists in fourteen chapters demonstrating essential examples of these aptamers and aptamer-nanomaterial assemblies, depending on the types of applications and biological systems. It also includes a separate chapter on the utilization of aptamers in real clinics and what will be required to achieve this significant goal. The book will be both appealing and useful to a broad audience, including biologists, bioscientists, and clinicians whose interests range from chemistry and biomedical engineering to cell and molecular biology and biotechnology. Weihong Tan is a Distinguished Professor of Chemistry and Biomedical Engineering at Hunan University, China and also a University of Florida Distinguished Professor and V.T. and Louis Jackson Professor of Chemistry at the University of Florida, USA. Xiaohong Fang is a Professor at the Institute of Chemistry, Chinese Academy of Sciences, China.
| Author | : Günter Mayer |
| Publisher | : Humana |
| Total Pages | : 242 |
| Release | : 2018-02-21 |
| Genre | : Science |
| ISBN | : 9781493949342 |
This volume provides protocol references covering recent developments in the aptamer field. Within the last decade, aptamers have become more and more popular, and their sophisticated biophysical properties together with their ability to be easily modified and, thus, adapted to various regimens makes them a very promising class of compounds. Divided into three sections, the book covers selection, a series of analytical methods to assess biophysical properties of aptamer-target interactions, as well as various applications of aptamers. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to follow, Nucleic Acid Aptamers: Selection, Characterization, and Application provides a state-of-the-art summary of recent developments in the aptamer field and will be a helpful resource for scientists in the life sciences working with aptamers as tools to elucidate biological systems.
| Author | : Katharina Urmann |
| Publisher | : Springer Nature |
| Total Pages | : 217 |
| Release | : 2020-07-31 |
| Genre | : Science |
| ISBN | : 3030540618 |
This book reviews the development, characterization and applications of aptamers in different areas of biotechnology ranging from therapeutics to diagnostics and protein purification. Hailed as chemical antibodies, these single-stranded nucleic acid receptors were predicted to supersede antibodies in traditional assays, such as ELISA, within a short time. While this has yet to happen, readers will find in this book a deep insight into the progress of aptamer technology and a critical discussion about the limitations that need to be overcome in order to find wider acceptance and use outside of the still relatively small aptamer-community. This book covers all aspects of aptamer generation and application for the aptamer-experienced reader and curious novice alike, with the addition of an industry perspective on the future of aptamer-use in biotechnology.
| Author | : Yiyang Dong |
| Publisher | : Springer Nature |
| Total Pages | : 470 |
| Release | : 2021-03-25 |
| Genre | : Science |
| ISBN | : 9813348380 |
This book outlines comprehensively the main medical uses of aptamers, from diagnosis to therapeutics in fourteen chapters. Pioneering topics covered include aptamer pharmaceuticals, aptamers for malign tumors, aptamers for personalized therapeutics and aptamers for point-of-care testing. The book offers an essential guide for medical scientists interested in developing aptamer-based schemes for better theranostics. It is therefore of interest for not only academic researchers, but also practitioners and medical researchers in various fields of medical science, medical research and bio-analytical chemistry.
| Author | : Giuseppe Domenico Arrabito |
| Publisher | : World Scientific |
| Total Pages | : 216 |
| Release | : 2017-09-25 |
| Genre | : Science |
| ISBN | : 1786343819 |
This book is intended for non-specialists and students, presenting a unique introduction to the field of DNA nanotechnology. The primary focus is on the extraordinary advantages of specificity and sensitivity obtained by integrating DNA nanostructures in bioanalytical devices.DNA Nanotechnology for Bioanalysis provides a concise and rigorous description for the fabrication of various types of functional nanostructures by optimized software-aided high-yield synthesis. Following this is the explanation of methods to decorate these nanostructures with molecules such as proteins, metal nanoparticles or bioorganic moieties covalently bonded onto DNA via self-assemblage processes. Also provided is a concise review on non-canonical DNA structures (such as G-quadruplexes) and their targeting by small molecules for applications in pharmacology. Finally, it describes the exciting applications of DNA nanostructures in life sciences and nanomedicine, including ultraspecific molecular delivery, control of cell behavior, analysis of cell lysate and DNA-based nano-tools for super-resolution sub-cellular imaging.
| Author | : Tom Shuster |
| Publisher | : |
| Total Pages | : 149 |
| Release | : 2019-09-06 |
| Genre | : |
| ISBN | : 9781536162936 |
This collection opens with a focus on recent advancements on the development of nucleic acid aptamers as alternative delivery systems for therapeutic oligonucleotides. Additionally, key examples of targeted delivery of the most common nucleic acid therapeutics, including small interfering RNAs, short hairpin RNAs, microRNAs and antisense oligonucleotides for a number of disorders are discussed. The following chapter deals with in vivo studies that were conducted with radiopharmaceuticals based on aptamers, the radionuclides used, the radiolabeling strategies, the chemical modifications of interest to improve their properties, and the main aptamers advantages and drawbacks for application as radiopharmaceuticals. In conclusion, the authors discuss the perspectives of using aptamers in various detection methods, their advantages and disadvantages, and the results of such work carried out through the present day.
| Author | : Jennifer Anne Martin |
| Publisher | : |
| Total Pages | : |
| Release | : 2010 |
| Genre | : |
| ISBN | : |
One aptamer displayed a dose-dependent reduction of the clotting time in buffer, with a 20 ... M aptamer concentration achieving a nearly complete antidote effect. A DNA microarray was used to truncate two of the aptamers to decrease the cost of aptamer synthesis for medical applications. The second group of experiments tested a simple aptamer-based square capillary cell affinity chromatography system for selective capture of target cancer cells from a flowing suspension. Aptamers were immobilized on the inner surface of the capillary through biotin-avidin chemistry, the extent of which was controlled by adjusting the aptamer concentration. The device captured target leukemia cells in higher amounts than nontarget cells under a variety of conditions, and the capture efficiency was optimized to retain>90% of cells. In addition, the system was used to capture two colon cancer cell lines by their respective aptamers at high efficiency. The capillary system could also detect stained cancer cells spiked in blood by imaging the length of the tubing and counting the fluorescent captured cells.
