Application Of Reflectance Confocal Microscopy In The Evaluation Of Murine Healthy And Lesional Skin PDF Download
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Author | : Jose Luis Egea |
Publisher | : |
Total Pages | : |
Release | : 2017 |
Genre | : |
ISBN | : |
Download Application of Reflectance Confocal Microscopy in the Evaluation of Murine Healthy and Lesional Skin Book in PDF, ePub and Kindle
Reflectance confocal microscopy (RCM) is a non-invasive imaging technology that allows real-time examination of the skin at nearly histologic resolution. It has been proved to be valuable in the clinics, but its application in animal models is limited. The main objectives were to identify characteristic confocal features in healthy murine skin, and evaluate the cutaneous alterations in the mouse model of oxazolone-induced dermatitis.A VivaScopeu00ae microscope was used to take horizontal optical sections of the skin from the stratum corneum to the dermis, up to the maximum optical depth of 250 u00b5m. Imaging was performed on healthy or lesional skin of male C57BL/6 mice. The dermatitis was induced in the depilated nape of sensitized mice by repeated topical challenges with oxazolone, and imaged 24 hours after each challenge.The major architectural and cellular features of each stratum of the epidermis and dermis were identified in healthy skin. However, the patterns were not as pronounced as those described in humans, and the dermo-epidermal junction was indistinct.Features of spongiotic dermatitis were visualized in the model. In the acute stage, there were areas of increased intercellular brightness (spongiosis), circumscribed oval hyporefractive areas (vesicles), and single or clusters of refractile round cells at the level of the stratum spinosum (exocytosis) and dermis. With chronicity, the honeycomb pattern was increased in thickness (acanthosis), and inflammatory cells were often observed with haphazardly arranged, highly refractile fibrilar fibers (fibrosis).RCM represents a powerful tool to analyze skin changes in animal models, and complements histopathology when studying dynamic processes (blood flow), or structures and lesions usually altered by histological processing. Additionally, its non-invasive nature enables the monitoring of skin lesions in the same animal, which is particularly useful in models intended to mimic a chronic disease.
Author | : Michael R. Hamblin |
Publisher | : Academic Press |
Total Pages | : 562 |
Release | : 2016-07-29 |
Genre | : Science |
ISBN | : 0128028599 |
Download Imaging in Dermatology Book in PDF, ePub and Kindle
Imaging in Dermatology covers a large number of topics in dermatological imaging, the use of lasers in dermatology studies, and the implications of using these technologies in research. Written by the experts working in these exciting fields, the book explicitly addresses not only current applications of nanotechnology, but also discusses future trends of these ever-growing and rapidly changing fields, providing clinicians and researchers with a clear understanding of the advantages and challenges of laser and imaging technologies in skin medicine today, along with the cellular and molecular effects of these technologies. Outlines the fundamentals of imaging and lasers for dermatology in clinical and research settings Provides knowledge of current and future applications of dermatological imaging and lasers Coherently structured book written by the experts working in the fields covered
Author | : Peter Altmeyer |
Publisher | : Springer |
Total Pages | : 480 |
Release | : 1992 |
Genre | : Medical |
ISBN | : |
Download Ultrasound in Dermatology Book in PDF, ePub and Kindle
Author | : T. Agner |
Publisher | : Karger Medical and Scientific Publishers |
Total Pages | : 172 |
Release | : 2016-02-04 |
Genre | : Medical |
ISBN | : 3318055867 |
Download Skin Barrier Function Book in PDF, ePub and Kindle
Although a very fragile structure, the skin barrier is probably one of the most important organs of the body. Inward/out it is responsible for body integrity and outward/in for keeping microbes, chemicals, and allergens from penetrating the skin. Since the role of barrier integrity in atopic dermatitis and the relationship to filaggrin mutations was discovered a decade ago, research focus has been on the skin barrier, and numerous new publications have become available. This book is an interdisciplinary update offering a wide range of information on the subject. It covers new basic research on skin markers, including results on filaggrin and on methods for the assessment of the barrier function. Biological variation and aspects of skin barrier function restoration are discussed as well. Further sections are dedicated to clinical implications of skin barrier integrity, factors influencing the penetration of the skin, influence of wet work, and guidance for prevention and saving the barrier. Distinguished researchers have contributed to this book, providing a comprehensive and thorough overview of the skin barrier function. Researchers in the field, dermatologists, occupational physicians, and related industry will find this publication an essential source of information.
Author | : Enzo Berardesca |
Publisher | : Springer Science & Business Media |
Total Pages | : 464 |
Release | : 2013-12-02 |
Genre | : Medical |
ISBN | : 3642321097 |
Download Non Invasive Diagnostic Techniques in Clinical Dermatology Book in PDF, ePub and Kindle
This book is a comprehensive but compact guide to the latest technical and technological developments in the growing field of non invasive diagnosis in clinical dermatology. Information is provided on the practical and technical characteristics of a wide range of equipment and methods for in vivo measurements that aid in the investigation of skin function, the evaluation of topically applied products and the monitoring of skin disease. Individual sections are devoted to imaging techniques, skin analysis, superficial skin analysis, skin mechanics, water and stratum corneum hydration and erythema and blood flow. All of the authors are experts in the field, with detailed knowledge of the techniques they describe. Non Invasive Diagnostic Techniques in Clinical Dermatology will be of value for all dermatologists, whether they are engaged in delivering patient care or in research programs, for cosmetic scientists and for biologists involved in skin research and product assessment.
Author | : Charles M. Balch |
Publisher | : Springer |
Total Pages | : 0 |
Release | : 2020-01-29 |
Genre | : Medical |
ISBN | : 9783030050689 |
Download Cutaneous Melanoma Book in PDF, ePub and Kindle
Written by the leading melanoma experts from the United States, Australia, and Europe, this new edition incorporates the clinical outcomes of more than 70,000 patients treated at major melanoma centers throughout the world and is the definitive and most authoritative textbook on melanoma used worldwide. Providing the most up-to-date and comprehensive information needed for the clinical management and scientific study of melanoma, Cutaneous Melanoma, 6th edition covers everything from precursors of melanoma to advanced stages of metastatic disease.
Author | : |
Publisher | : Academic Press |
Total Pages | : 521 |
Release | : 2003-01-03 |
Genre | : Science |
ISBN | : 0080925359 |
Download Cell Biological Applications of Confocal Microscopy Book in PDF, ePub and Kindle
This volume of the acclaimed Methods in Cell Biology series provides specific examples of applications of confocal microscopy to cell biological problems. It is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. An integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM). Includes detailed protocols accessible to new users Details how to set up and run a "Confocal Microscope Core Facility" Contains over 170 figures
Author | : Warren R. Sanborn |
Publisher | : |
Total Pages | : 327 |
Release | : 2005 |
Genre | : Cytodiagnosis |
ISBN | : 9789290213956 |
Download Fluorescence Microscopy for Disease Diagnosis and Environmental Monitoring Book in PDF, ePub and Kindle
Laboratories should be more aware of the advantages of using fluorescence microscopy. This manual provides information on the principles of fluorescence microscopy and practical advice on the preparation of samples for many simple applications for diagnosing disease and monitoring environmental contamination using a fluorescence microscope. The publication puts emphasis on procedures for direct, rapid identification of microorganisms causing a disease. The practical steps of indirect immunofluorescence microscopy for the diagnosis of noncommunicable diseases are also considered.
Author | : Francesco S. Pavone |
Publisher | : Taylor & Francis |
Total Pages | : 465 |
Release | : 2016-04-19 |
Genre | : Science |
ISBN | : 1439849153 |
Download Second Harmonic Generation Imaging Book in PDF, ePub and Kindle
Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical a
Author | : Ji-Xin Cheng |
Publisher | : Elsevier |
Total Pages | : 612 |
Release | : 2021-12-04 |
Genre | : Science |
ISBN | : 0323903371 |
Download Stimulated Raman Scattering Microscopy Book in PDF, ePub and Kindle
Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics