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| Author | : Stanley T. Crooke |
| Publisher | : CRC Press |
| Total Pages | : 848 |
| Release | : 2007-07-25 |
| Genre | : Medical |
| ISBN | : 1439832501 |
Download Antisense Drug Technology Book in PDF, ePub and Kindle
Extensively revised and updated, Antisense Drug Technology: Principles, Strategies, and Applications, Second Edition reflects the logarithmic progress made in the past four years of oligonucleotide-based therapies, and, in particular, antisense therapeutics and research. Interpreting lessons learned from the clinical trials of first generati
| Author | : Conrad Lichtenstein |
| Publisher | : IRL Press |
| Total Pages | : 334 |
| Release | : 1997 |
| Genre | : Antisense DNA. |
| ISBN | : 9780199635832 |
Download Antisense Technology Book in PDF, ePub and Kindle
Antisense technology is a powerful procedure that permits the controlled silencing of a specific gene for investigations of mRNA and protein function. This valuable text provides proven step-by-step protocols for antisense techniques in a range of different organisms and cell culture systems. In addition it discusses the potential benefits and problems for various antisense methods which complement gene knock-out experiments. The book includes antisense techniques such as: analysis of nucleic acid structures; measurement and evaluation of antisense effects; selection, preparation and the use of antisense oligonucleotides; in vitro RNA transcription; construction strategies, testing and optimization of catalytic antisense RNAs based on hammerhead ribozymes; synthesis and evaluation of 2-5 A- antisense chimeras for targeted degradation of RNA. The application of these technologies are then described, with chapters on antisense techniques in IDictyostelium, plants and the medical uses and benefits of antisense sense technology both in vitro and in vivo. Finally, non-antisense effects of oligonucleotides, "anti-sense rescue", shotgun antisense mutagenesis, and in vivo, in vitro and in situ detection of sense: antisense duplexes by structure-specific anti-RNA antibodies are discussed. The numerous hints and tips for success and advice on trouble shooting, make this authoritative text invaluable to all those researchers who work on gene expression, RNA transcription, or protein function.
| Author | : |
| Publisher | : Elsevier |
| Total Pages | : 614 |
| Release | : 1999-11-01 |
| Genre | : Science |
| ISBN | : 0080496709 |
Download Antisense Technology, Part A, General Methods, Methods of Delivery, and RNA Studies Book in PDF, ePub and Kindle
Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation. For example, methods that inhibit gene expression permit studies which probe the normal function of a specific product within a cell. Such methodology can be used in many disciplines such as pharmacology, oncology, genetics, cell biology, developmental biology, molecular biology, biochemistry, and neurosciences. This volume will be a truly important tool in biomedical-oriented research. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
| Author | : Stanley T. Crooke |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 646 |
| Release | : 2012-12-06 |
| Genre | : Medical |
| ISBN | : 3642587852 |
Download Antisense Research and Application Book in PDF, ePub and Kindle
Antisense technology may result in dramatic changes in the therapy of many diseases and may provide tools to dissect pharmacological processes and to confirm the roles of various genes. In this volume, progress in the understanding of antisense technology and its use in creating new drugs is discussed. Potential caveats, pitfalls and limitations of the technology are also presented. In the next few years the pace at which new molecular targets will be identified will increase exponentially as the sequencing of the human genome and of other genomes proceeds.
| Author | : |
| Publisher | : Elsevier |
| Total Pages | : 683 |
| Release | : 1999-11-10 |
| Genre | : Science |
| ISBN | : 0080496717 |
Download Antisense Technology, Part B: Applications Book in PDF, ePub and Kindle
Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation. For example, methods which inhibit gene expression permit studies probing the normal function of a specific product within a cell. Such methodology can be used in many disciplines such as pharmacology, oncology, genetics, cell biology, developmental biology, molecular biology, biochemistry, and neurosciences. This volume will be a truly important tool in biomedically-oriented research. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences.
| Author | : Ronald A. Leslie |
| Publisher | : Oxford University Press, USA |
| Total Pages | : 280 |
| Release | : 1999 |
| Genre | : Medical |
| ISBN | : |
Download Antisense Technology in the Central Nervous System Book in PDF, ePub and Kindle
"Antisense Technology in the Central Nervous System describes the promises and the pitfalls of this approach, and compares it to other approaches. The practical experience of leading researchers is combined with a thorough description of the theoretical foundation of the technique, making the book ideal for researchers and clinicians alike."--BOOK JACKET.
| Author | : Stanley T. Crooke |
| Publisher | : CRC Press |
| Total Pages | : 620 |
| Release | : 1993-05-27 |
| Genre | : Medical |
| ISBN | : 9780849347054 |
Download Antisense Research and Applications Book in PDF, ePub and Kindle
Antisense Research and Applications is a comprehensive review of oligonucleotide research covering molecular biological advances in this field, the current status of antisense drug research, and strategies for future research and therapeutic applications. In bringing together the latest research from an array of authoritative scientists, Antisense Research and Applications provides an integrated conceptual basis for considering oligonucleotide therapeutics. Topics covered in the 32 chapters of this book include nucleic acid structure and function, antisense RNA, medicinal chemistry of oligonucleotides, analogs, pharmacokinetics and toxicology, and activities of current antisense drugs. This volume addresses advances in a broad range of disciplines and is an excellent resource for basic researchers and applied investigators in pharmaceutical laboratories and in such fields as biochemistry and molecular biology.
| Author | : |
| Publisher | : |
| Total Pages | : |
| Release | : 1993 |
| Genre | : |
| ISBN | : |
Download Antisense technology Book in PDF, ePub and Kindle
| Author | : Cy A. Stein |
| Publisher | : Wiley-Liss |
| Total Pages | : 556 |
| Release | : 1998-03-19 |
| Genre | : Medical |
| ISBN | : |
Download Applied Antisense Oligonucleotide Technology Book in PDF, ePub and Kindle
Applied Antisense Oligonucleotide Technology provides the basic concepts as well as the practical concerns associated with the use of antisense oligonucleotides to modify gene expression. Chapters include oligonucleotide chemistry, DNA triplex formation, delivery mechanisms, pharmocokinetics, toxicity, oligonucleotides.
| Author | : Macy Rose |
| Publisher | : Callisto Reference |
| Total Pages | : 0 |
| Release | : 2023-09-26 |
| Genre | : Science |
| ISBN | : 9781641167857 |
Download Antisense Technology: Methods of Delivery and RNA Studies Book in PDF, ePub and Kindle
Antisense RNA (asRNA) refers to a type of single-stranded RNA. It hybridizes with protein coding messenger RNA (mRNA) and complements it to inhibit its translation into a protein. Eukaryotes and prokaryotes, both have naturally occuring asRNAs in them. They can be divided into two types including short and long non-coding RNAs (ncRNAs). asRNAs primarily control the gene expression and are widely utilized as tools for gene knockdown. Antisense technology offers effective experimental methods for examining gene function and gene regulation by modifying gene expression in mammalian cells. Many RNA-based antisense therapies are being developed currently. However, effective delivery of these therapies to target tissues remains the primary barrier in their clinical application. Antisense technology has a wide range of applications in the treatment of numerous diseases such as viral infections, cancer, and immunological, cardiovascular, ocular and neurological diseases. This book contains some path-breaking studies in antisense technology. It consists of contributions made by international experts. This book is a vital tool for all researching or studying antisense RNA as it gives incredible insights into its design and delivery.
