Animal Cell Culture Techniques PDF Download
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| Author | : John M. Davis |
| Publisher | : John Wiley & Sons |
| Total Pages | : 389 |
| Release | : 2011-03-16 |
| Genre | : Science |
| ISBN | : 0470975636 |
Download Animal Cell Culture Book in PDF, ePub and Kindle
This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.
| Author | : R. Ian Freshney |
| Publisher | : Wiley-Liss |
| Total Pages | : 510 |
| Release | : 1993-12-29 |
| Genre | : Science |
| ISBN | : 9780471589662 |
Download Culture of Animal Cells Book in PDF, ePub and Kindle
This masterful third edition of Freshney's Culture of Animal Cells updates and considerably expands the scope of its predecessor and still enables both the novice and the experiences researcher to apply the basic and more sophisticated techniques of tissue culture. New Topics covered include: the use of molecular techniques in cell culture, such as DNA fingerprinting, fluorescence in situ hybridization, and chromosome painting cell interactions in cell culture new methods for separating cells new or refined methods for accessing cytotoxicity, viability, and mutagenicity experimental details for culture of specialized cells types not covered in previous editions new or refined techniques for visualizing clues, including time-lapse photography and confocal microscopy The revised and expanded third edition offers the following features: over 350 new reference to the primary literature an international list of cell banks an international listing of reagants and commercial supplies a subject index a glossary Also available: 0471169021 Culture of Animal Cells: A Multimedia Guide CD-ROM $150 est. From the reviews: "I strongly recommend this volume for any laboratory wishing to culture mammalian cells" - Biotechnology "It is not very often that it is possible to say of a book, 'I don't know how I managed without it previously.' Here is such a book" - Cell Biology International Reports
| Author | : Martin Clynes |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 624 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 3642804128 |
Download Animal Cell Culture Techniques Book in PDF, ePub and Kindle
Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.
| Author | : |
| Publisher | : Academic Press |
| Total Pages | : 389 |
| Release | : 1998-07-03 |
| Genre | : Science |
| ISBN | : 0080859550 |
Download Animal Cell Culture Methods Book in PDF, ePub and Kindle
This volume provides complete and thorough coverage of the classical and state-of-the-art methods used in cell culture. It also includes basic principles used in the selection of cells for specific scientific study, as well as analytical and procedural techniques. Key Features * Reviews basic principles of cell culture * Gives options and techniques on how to look at cells
| Author | : Maureen A. Harrison |
| Publisher | : Cambridge University Press |
| Total Pages | : 178 |
| Release | : 1997-10-13 |
| Genre | : Science |
| ISBN | : 9780521574969 |
Download General Techniques of Cell Culture Book in PDF, ePub and Kindle
Concise introduction to a major technique of cell biology laboratories for those new to the field.
| Author | : Mell Carter & |
| Publisher | : Scientific e-Resources |
| Total Pages | : 316 |
| Release | : 2019-10-15 |
| Genre | : |
| ISBN | : 1839471573 |
Download Animal Cell Culture Book in PDF, ePub and Kindle
Cell culture refers to the removal of cells from an animal or plant and their subsequent growth in a favourable artificial environment. The cells may be removed from the tissue directly and disaggregated by enzymatic or mechanical means before cultivation, or they may be derived from a cell line or cell strain that has already been established. Stem cells retain the capacity to self renew as well as to produce progeny with a restricted mitotic potential and restricted range of distinct types of differentiated cell they give rise to. The formation of blood cells, also called haematopoiesis, is the classical example of concept of stem cells. Animal cell and tissue culture is an integral part of biotechnology and this book covers all the aspects of animal cell culture. Animal cells are used for making new vaccines, specific animal proteins such as intergerons, blood factors and hormones, monoclonal antibodies for use as diagnostic and therapeutics, gene probes as diagnostic too, enzymes and last but not the least many new and important compounds. This book contains eleven Chapters, which deal with historic developments, laboratory design, sterilization procedures and various facets of animal cell culture. This includes preservation, characterizations, storage and transport of cells, their monitoring and technologies for cell banking.
| Author | : A Wilson Aruni |
| Publisher | : MJP Publisher |
| Total Pages | : 219 |
| Release | : 2019-06-10 |
| Genre | : Fiction |
| ISBN | : |
Download Animal Tissue Culture Book in PDF, ePub and Kindle
Cell and tissues culture has been one of the first and foremost techniques paving for recent cutting-edge technologies such as vaccinology, monoclonal antibody production, therapeutic cloning, stem cell technology, etc. It has played a substantial role in the developments of health care and prophylactics industries, thus serving the mankind. It has made the dream of producing cost-effective prophylactics, diagnostics and therapeutics come true and affordable. In the recent past, with the explosion of knowledge in the field of biotechnology, intensive research in being carried out, where undergraduate and post-graduate courses are being offered in this field. Even through more emphasis is being given to theory, a dearth of practical knowledge is lacking due to paucity of established tissue culture facilities.
| Author | : Michael Butler |
| Publisher | : Garland Science |
| Total Pages | : 299 |
| Release | : 2004-08-02 |
| Genre | : Medical |
| ISBN | : 1135325375 |
Download Animal Cell Culture and Technology Book in PDF, ePub and Kindle
Animal cell culture is an important laboratory technique in the biological and medical sciences. It has become an essential tool for the study of most biochemical and physiological processes and the use of large-scale animal cell culture has become increasingly important to the commercial production of specific compounds for the pharmaceutical industry. This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for undergraduates, graduates and experienced researchers. Reflecting the latest developments and trends in the field, the new topics include the latest theory of the biological clock of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation, and the humanization of antibodies for therapeutic use.
| Author | : Jeffrey W. Pollard |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 477 |
| Release | : 1997 |
| Genre | : Animals |
| ISBN | : 0896034410 |
Download Basic Cell Culture Protocols Book in PDF, ePub and Kindle
Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, 2nd ed. offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. Like the first edition, this book should be in every cell culture laboratory and be of use to all who use cell cultures in research.
| Author | : Ralf Pörtner |
| Publisher | : Humana |
| Total Pages | : 0 |
| Release | : 2016-08-23 |
| Genre | : Science |
| ISBN | : 9781493963188 |
Download Animal Cell Biotechnology Book in PDF, ePub and Kindle
Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterisation. Part II addresses tools for process and medium optimization as well as microcarrier technology while Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques. Part IV details cultivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Animal Cell Biotechnology: Methods and Protocols, Third Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.
