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Analytical Ultracentrifugation V

Analytical Ultracentrifugation V
Author: H. Cölfen
Publisher: Springer
Total Pages: 233
Release: 2003-07-01
Genre: Technology & Engineering
ISBN: 3540487034

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The basis for this volume is the 11th Symposium on Analytical Ultracentrifugation held in March 25-26, 1999 at the University of Potsdam, Germany. This book presents a comprehensive collection of 33 contributions from leading scientists in this field including: Technical and methodological innovations.- Innovations in data analysis.- Hydrodynamics/Modelling.- Synthetic polymers, colloids and supramolecular systems.- Biological systems.- Interacting systems and assemblies. In contrast to the increasing significance of analytical ultracentrifugation, related modern books are very rare. Therefore, this volume will be a helpful source of information to anyone who wants to catch up with the most recent developments and results related to this important analytical method.


Sedimentation Velocity Analytical Ultracentrifugation

Sedimentation Velocity Analytical Ultracentrifugation
Author: Peter Schuck
Publisher: CRC Press
Total Pages: 271
Release: 2017-09-22
Genre: Science
ISBN: 1351976842

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Analytical ultracentrifugation is one of the most powerful solution techniques for the study of macromolecular interactions, to define the number and stoichiometry of complexes formed, and to measure affinities ranging from very strong to very weak and repulsive. Building on the data analysis tools described in the volume Sedimentation Velocity Analytical Ultracentrifugation: Discrete Species and Size-Distributions of Macromolecules and Particles, and the experimental and instrumental aspects in the first volume Basic Principles of Analytical Ultracentrifugation, the present volume Sedimentation Velocity Analytical Ultracentrifugation: Interacting Systems is devoted to the theory and practical data analysis of dynamically coupled sedimentation processes. This volume is designed to fill a gap in biophysical methodology to provide a framework that builds on the fundamentals of the highly developed traditional methods of analytical ultracentrifugation, updated with current methodology and from a viewpoint of modern applications. It will be an invaluable resource for researchers and graduate students interested in the application of analytical ultracentrifugation in the study of interacting systems, such as biological macromolecules, multi-protein complexes, polymers, or nanoparticles.


Basic Principles of Analytical Ultracentrifugation

Basic Principles of Analytical Ultracentrifugation
Author: Peter Schuck
Publisher: CRC Press
Total Pages: 318
Release: 2016-01-05
Genre: Medical
ISBN: 1498751164

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Analytical ultracentrifugation (AUC) can supply rich information on the mass, shape, size distribution, solvation, and composition of macromolecules and nanoscopic particles. It also provides a detailed view of their reversible single- or multi-component interactions over a wide range of affinities. Yet this powerful technique has been hard to mast


Analytical Ultracentrifugation

Analytical Ultracentrifugation
Author: Susumu Uchiyama
Publisher: Springer
Total Pages: 520
Release: 2016-05-13
Genre: Science
ISBN: 443155985X

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This book introduces analytical ultracentrifugation (AUC) as a whole, covering essential theoretical and practical aspects as well as its applications in both biological and non-biological systems. Comprehensive characterizations of macromolecules in a solution are now routinely required not only for understanding the solution system but also for producing a solution with better properties. Analytical ultracentrifugation is one of most powerful and reliable techniques for studying the biophysical behavior of solutes in solution. In the last few years, there have been steady advances made in hardware, software, and applications for AUC. This book provides chapters that cover everything essential for beginners to the most advanced users and also offer updated knowledge of the field on advances in hardware, software, and applications. Recent development of hardware described in this book covers new detection systems that give added dimensions to AUC. Examples of data analysis with essential theoretical explanations for advanced and recently updated software are also introduced. Besides AUC of biological systems including membrane proteins and biopharmaceuticals, AUC applications for non-biological questions are included. AUC studies under non-ideal conditions such as highly concentrated solutions and solutions with high salt concentration are also included. The contributors to this book are leading researchers in the fields of solution biophysics and physical chemistry who extensively employ AUC analysis for their research. From this published work, one can gain new and comprehensive knowledge of recent AUC analysis.


Analytical Ultracentrifugation

Analytical Ultracentrifugation
Author:
Publisher: Academic Press
Total Pages: 617
Release: 2015-09-24
Genre: Science
ISBN: 0128029099

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Analytical Ultracentrifugation, the latest volume in Methods in Enzymology, focuses on analytical ultracentrifugation. The scope of this technique has greatly expanded in recent years due to advances in instrumentation, algorithms and software. This volume describes the latest innovations in the field and in the applications of analytical ultracentrifugation in the analysis of macromolecules, macromolecular assemblies, and biopharmaceuticals. Timely contribution that describes a rapidly changing field Leading researchers in the field Broad coverage: instrumentation, basic theory, data analysis, and applications


Analytical Ultracentrifugation VIII

Analytical Ultracentrifugation VIII
Author: Christine Wandrey
Publisher: Springer
Total Pages: 0
Release: 2010-02-12
Genre: Science
ISBN: 9783642067402

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The 14th International Symposium on Analytical Ultracentrifugation was held in March 2005 at the École Polytechnique Fédérale de Lausanne in Switzerland. This book presents a comprehensive collection of 21 contributions from leading scientists in this field covering a broad spectrum of topics and presenting recent progress concerning instrumentation, data analysis and modeling, biological systems, particles, colloids, synthetic macromolecules, interacting systems. Analytical Ultracentrifugation is becoming increasingly important in both academic and industrial applications. Due to the versatility of this fascinating and powerful technique, information and original publications are widespread and comprehensive collections are rare. Therefore, this volume presents a valuable source for biologists, chemists, materials scientists, and physicists interested in most recent information, results and development related to this important analytical method.


Analytical Ultracentrifugation VI

Analytical Ultracentrifugation VI
Author: W. Borchard
Publisher: Springer Science & Business Media
Total Pages: 168
Release: 2002-02-11
Genre: Science
ISBN: 354042489X

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This volume includes 20 contributions of the 12th meeting on Analytical Ultracentrifugation from March 1-2, 2001 in Duisburg, Germany. Various fields of ultracentrifugation are covered concerning research problems in biochemistry, biophysical chemistry and macromolecular chemistry as well as interacting systems. New investigations concerning the sedimentation theory are presented. The phase transition of gels is dealt with, as is the sedimentation-diffusion equilibrium of gels. One section contains the hydrodynamics of biopolymers.


Analytical Ultracentrifugation

Analytical Ultracentrifugation
Author: David Scott
Publisher: Royal Society of Chemistry
Total Pages: 613
Release: 2007-12-16
Genre: Science
ISBN: 1847552617

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Analytical ultracentrifugation has become an increasingly important technique for monitoring the size and shape of biological macromolecules. Analytical Ultracentrifugation: Techniques and Methods contains contributions from experts in the field, bringing together the multitude of developments that have taken place in instrumentation and analysis over the past decade into a single volume. This book covers the latest methods in analysis along with an extensive introduction for the novice user. Analysis methods in both sedimentation velocity and sedimentation equilibrium are discussed at length. Protein, protein/DNA, membrane proteins and polymer systems are also explored, along with software developments and non-ideality.


Sedimentation Velocity Analytical Ultracentrifugation

Sedimentation Velocity Analytical Ultracentrifugation
Author: Peter Schuck
Publisher: CRC Press
Total Pages: 267
Release: 2016-11-17
Genre: Medical
ISBN: 1498768954

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A follow-up to the experimental and instrumental aspects described in Basic Principles of Analytical Ultracentrifugation, the volume Sedimentation Velocity Analytical Ultracentrifugation: Discrete Species and Size-Distributions of Macromolecules and Particles describes the theory and practice of data analysis. Mathematical models for the sedimentation process and the evolution of detected signals are developed in a comprehensive framework, jointly with the description of current and historical strategies for how to extract from noisy experimental data the physical parameters of interest, such as size, mass, and shape, composition, and polydispersity of sedimenting particles. The methods are extensively illustrated, and supported with practical applications, as well as cross-references where to find the methods in the public domain software SEDFIT and SEDPHAT. The systems covered are discrete or polydisperse mixtures of sedimenting molecules or particles in dilute solution, such as proteins and other biomolecules and their stable complexes, man-made polymers, and nanoparticles, observed in different optical systems. A useful reference for researchers and graduate students of macromolecular disciplines, these methods form the essential foundation for the analysis of dynamic interacting systems, which are covered in the volume Sedimentation Velocity Analytical Ultracentrifugation: Interacting Systems. Software referenced in the book is available for download at: https://sedfitsedphat.nibib.nih.gov/default.aspx


Analytical Ultracentrifugation VII

Analytical Ultracentrifugation VII
Author: M. D. Lechner
Publisher: Springer Science & Business Media
Total Pages: 180
Release: 2004-11-24
Genre: Science
ISBN: 9783540202035

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This volume includes 19 contributions to the 13th International Symposium on Analytical Ultracentrifugation which took place at the university of Osnabrück on March 6th and 7th, 2003. The contributions from leading scientists cover a broad spectrum of topics concerning: Technical Methods, Data Analysis, Innovations; Polymers, Colloids, Supramolecular Systems; Biological and Interaction Systems; Hydrodynamics and Modelling. Due to the increasing significance of Analytical Ultracentrifugation for both scientific and technical applications, this book will be an essential source of information with respect to recent developments and results related to this important analytical method.