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Analytical Techniques in Immunochemistry

Analytical Techniques in Immunochemistry
Author: Terry M. Phillips
Publisher: CRC Press
Total Pages: 370
Release: 1991-12-23
Genre: Science
ISBN: 9780824784775
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Explores the principles, and presents step-by-step recipes for isolating and measuring biological materials using modern immunochemical methods, including such new procedures as high-performance immunoaffinity chromatography, capillary electrophoresis, and blotting. Also includes clear drawings of

Physical and Chemical Methods

Physical and Chemical Methods
Author: Curtis A. Williams
Publisher: Academic Press
Total Pages: 480
Release: 2014-05-12
Genre: Medical
ISBN: 1483222993
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Methods in Immunology: Volume II, Physical and Chemical Methods is a collection of papers dealing with electrophoresis, analytical ultracentrifugation, dialysis, ultrafiltration, cellulose ion exchangers, and chromatographic separation of macromolecules on porous gels. Some papers explain the applications of radioisotopes, optical analysis, and chemical analysis of proteins, carbohydrates, lipids, and nucleic acid. One paper describes the theory of electro-migration. Factors such as electrical charge or frictional coefficients govern the rate of migration of charged particles in an electric field. The differences found in their velocities can be used to separate substances or analyze them. Mobility is a characteristic property of molecules and can also be influenced by the composition of the medium or solution. Dialysis separates solvents too large to diffuse through a barrier from smaller solutes; ultrafiltration (reverse osmosis) forces solvent and solutes up to a certain critical size through the barrier by a high pressure on one side. The book notes that the membrane never becomes plugged in dialysis because of some opposite movement of the solvent. Another paper points out that the significance of radioactive tracers in immunochemistry employed to identify and label macromolecules functioning as antigens and antibodies. The collection can prove valuable to bio-chemists, cellular biologists, micro-biologists, developmental biologists, and scientists involved in immunological research.

Methods of Immunological Analysis, Methods of Immunological Analysis

Methods of Immunological Analysis, Methods of Immunological Analysis
Author: Rene Masseyeff
Publisher: Wiley-Blackwell
Total Pages: 752
Release: 1992-12-11
Genre: Medical
ISBN: 9783527279067
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Everyone conducting analyses using immunological methods should read METHODS OF IMMUNOLOGICAL ANALYSIS. Now available! Three detailed volumes supplying a complete practice-oriented introduction to immunoanalysis. A set in themselves, they provide an extraordinarily comprehensive and up-to-date depiction of these methods. Both specialists and non-specialists will appreciate the broad scope of this set: It covers a wide area of the life sciences, chemistry and medicine, adjusted to the extensive applicability of immunoanalytical techniques. We at VCH feel that there is a definite need for this indispensable new series. Immunoanalysis is widely practised by analysts whose interests or training are not primarily in immunology. They clearly need the guidance that a good reference work provides. Furthermore, the range of immunochemical methods itself is so broad and its techniques so varied that even specialist immunologists and immunochemists find it difficult to assess different possible analytical approaches. They, too, need the support of an extensive reference work. Save 20% by subscribing to the series by 30 June 1993!

Analytical Techniques in Biosciences

Analytical Techniques in Biosciences
Author: Chukwuebuka Egbuna
Publisher: Academic Press
Total Pages: 302
Release: 2021-10-21
Genre: Science
ISBN: 0128227990
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Analytical Techniques in Biosciences: From Basics to Applications presents comprehensive and up-to-date information on the various analytical techniques obtainable in bioscience research laboratories across the world. This book contains chapters that discuss the basic bioanalytical protocols and sample preparation guidelines. Commonly encountered analytical techniques, their working principles, and applications were presented. Techniques, considered in this book, include centrifugation techniques, electrophoretic techniques, chromatography, titrimetry, spectrometry, and hyphenated techniques. Subsequent chapters emphasize molecular weight determination and electroanalytical techniques, biosensors, and enzyme assay protocols. Other chapters detail microbial techniques, statistical methods, computational modeling, and immunology and immunochemistry. The book draws from experts from key institutions around the globe, who have simplified the chapters in a way that will be useful to early-stage researchers as well as advanced scientists. It is also carefully structured and integrated sequentially to aid flow, consistency, and continuity. This is a must-have reference for graduate students and researchers in the field of biosciences. Presents basic analytical protocols and sample-preparation guidelines Details the various analytical techniques, including centrifugation, spectrometry, chromatography, and titrimetry Describes advanced techniques such as hyphenated techniques, electroanalytical techniques, and the application of biosensors in biomedical research Presents biostatistical tools and methods and basic computational models in biosciences

Immunochemical Techniques Laboratory Manual

Immunochemical Techniques Laboratory Manual
Author: John Goers
Publisher:
Total Pages: 212
Release: 1993
Genre: Medical
ISBN:
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This is a laboratory manual for a course in immunochemical techniques designed to introduce students to the subject, enabling them to understand the wider applications of the techniques they are using. The text is written with easy-to-follow protocols for immunological techniques used in modern research labs and biochemical companies.

Immunochemistry of Solid-Phase Immunoassay

Immunochemistry of Solid-Phase Immunoassay
Author: John E. Butler
Publisher: CRC Press
Total Pages: 334
Release: 1991-07-23
Genre: Science
ISBN: 9780849353949
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Immunochemistry of Solid-Phase Immunoassay fills a niché in the field of immunoassay and immunology. Although solid-phase immunoassay constitutes a major technology in biology and medicine, there is no comprehensive source devoted to the immunochemical principles involved. As a result, this book will benefit students, technicians, and researchers who use this technology, as well as immunodiagnostic and biotech companies who develop the technology. The book is not a methods manual; instead, it incorporates the concepts, data, and opinions of more than 25 investigators working in this field. Topics discussed include: the chemistry of solid-phases, the behavior or antibodies and antigens on solid phases, membrane solid-phases, reaction kinetics, antigen quantitation, enzyme systems, photophysics, immunochemical considerations in data analyses, multianalyte assays and occupancy concepts, antibody quantitation, streptavidin, a review of data analysis software, and solid-phase peptide immunoassay.

Handbook of Immunochemistry

Handbook of Immunochemistry
Author: Miroslav Ferencik
Publisher: Springer Science & Business Media
Total Pages: 529
Release: 2012-12-06
Genre: Science
ISBN: 9401115524
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The book appeared in two previous Slovak editions for university students in Czechoslovakia. This edition presents a completely new version updated according to recent advances not only in immunochemistry and essential immunology but also in molecular biology, biochemistry and molecular genetics. The scope of the book is considerable since the goal was to cover the field of immunochemistry from the widest point of view including both the topic and methods of contemporary immunochemistry. Each chapter provides basic information on a specific subtopic, clearly and understandab ly, and presents principles of individual immunochemical methods. I am confident that the book will fill the gap between the books on essential immunology and highly specialised books on individual areas of immunoche mistry (e. g. on antibodies, antigens, numerous immunochemical techniques, etc. ). It may also prove useful for beginning investigators from different biological and medical fields as it supplies basic information needed for solving their scientific problems by immunochemical approaches. I do hope that readers will find the text stimulatory and pleasury to read. I wish to thank all colleagues and friends for supplying their own results, suggestions and for their encouraging comments. My thanks go also to the editors and publishers for their valuable contribution to the preparation of the book. 1 Introduction The term immunochemistry was coined by the Swedish chemist ARRHENIUS who used it for the first time in his lectures in 1907.

Immunohistochemistry

Immunohistochemistry
Author: Trung Nguyen
Publisher: Cambridge University Press
Total Pages: 283
Release: 2022-07-07
Genre: Medical
ISBN: 1009116053
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This book will enable practitioners to understand the many complex intricacies of immunohistochemistry (IHC) and make best use of this powerful analytical tool. Providing a thorough grounding in the fundamentals of immunohistochemistry, the book includes several chapters on robotics and automation technology, giving key information on the design of machines and tips to maximise workflow efficiencies. The relationship between IHC and molecular pathology is explained clearly, demonstrating the increasing impact on personalized medicine and targeted therapies for cancer patients. The staining protocol is deconstructed, allowing the reader to adapt it for a variety of diagnostic and research applications. Written by experts at the forefront of hospital immunohistochemistry, there is a strong emphasis on practical guidance on a range of techniques as well as troubleshooting of common problems driven by the authors' experiences. Extensively illustrated with high-quality colour images, this is an invaluable resource to all pathology practitioners utilising the technique.

Immunohistochemistry: Basics and Methods

Immunohistochemistry: Basics and Methods
Author: Igor B. Buchwalow
Publisher: Springer Science & Business Media
Total Pages: 153
Release: 2010-01-26
Genre: Medical
ISBN: 3642046096
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This concise yet comprehensive guide to the methods and protocols of immunohistochemistry covers established techniques and current developments in the field such as the use of epitope tags, multiple immunolabeling and diagnostic immunohistochemistry.