Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Analysis Of Maternal Effect Genes And Daf 3 In Caenorhabditis Elegans Dauer Formation PDF full book. Access full book title Analysis Of Maternal Effect Genes And Daf 3 In Caenorhabditis Elegans Dauer Formation.
| Author | : Allison Renee Koweek |
| Publisher | : |
| Total Pages | : 238 |
| Release | : 1997 |
| Genre | : Caenorhabditis elegans |
| ISBN | : |
| Author | : Jennifer R. Berman |
| Publisher | : |
| Total Pages | : 402 |
| Release | : 2005 |
| Genre | : |
| ISBN | : |
Finally, we further explore the role of daf-12 on lifespan and thermotolerance, as well as examine the effects of environmental perturbation on the lifespan of germline deficient animals.
| Author | : Annette Orene Zager Estevez |
| Publisher | : |
| Total Pages | : 264 |
| Release | : 1997 |
| Genre | : Caenorhabditis elegans |
| ISBN | : |
When starved and overcrowded, the free-living soil nematode C. elegans can form a developmentally arrested dispersal stage called the dauer larva. This process is partially mediated by TGF-$\beta$-induced signalling. Mutations in the daf-8 gene result in constitutive dauer larva formation (Daf-c), reduced fecundity, and some embryonic and larval lethality. This gene has been cloned by transposon tagging. A region flanking a transposon insertion in daf-8 hybridizes to two overlapping cosmids. Sequence analysis of cosmid subclones identifies a Smad family protein shown by mutation site analysis to be encoded by daf-8. Mutational and phenotypic analyses have defined amino acids 389-415 as a region of the protein important for embryonic and larval viability. A cosmid fragment used to probe a developmental northern hybridized to 1.3 kb and 1.6 kb transcripts. The smaller transcript is expressed most strongly in L1 and dauer larvae, two stages in which major developmental decisions are made. The daf-7 gene encodes a TGF-$\beta$ ligand that putatively binds the DAF-4/DAF-1 receptor complex, regulating dauer larva formation. All daf-1, daf-4 and daf-7 mutants are Daf-c. Additionally, daf-4 mutants have phenotypes not shared in common with daf-7 mutants. Another C. elegans TGF-$\beta$ ligand, CeBMP, identified by degenerate PCR, may be involved in regulation of some of the daf-4-specific phenotypes. Since TGF-$\beta$ ligands function as dimers, CeBMP may dimerize with DAF-7 to regulate dauer larva formation. I propose a model by which dauer larva formation is regulated by DAF-7/CeBMP binding to the DAF-4 and DAF-1 receptors. DAF-1 phosphorylates DAF-8 which along with DAF-14, antagonizes the activity of DAF-3 to initiate growth and prevent dauer larva formation.
| Author | : Joan Marsh |
| Publisher | : John Wiley & Sons |
| Total Pages | : 344 |
| Release | : 1994-08-16 |
| Genre | : Medical |
| ISBN | : |
Connects classical cellular descriptive studies with more recent work on the molecular and genetic aspects regarding germline development. Prominent scientists discuss research on a range of organisms including insects, worms, birds, fish, amphibia, mammals and green algae. Specification of germ cells, their migration to the gonads and subsequent interactions with the soma and evolutionary factors of their segregation are among the topics covered.
| Author | : Kari Anne Haus |
| Publisher | : |
| Total Pages | : 52 |
| Release | : 2005 |
| Genre | : |
| ISBN | : |
| Author | : Ryuji Minasaki |
| Publisher | : |
| Total Pages | : 59 |
| Release | : 2007 |
| Genre | : |
| ISBN | : |
| Author | : Donald L. Riddle |
| Publisher | : Firefly Books |
| Total Pages | : 1252 |
| Release | : 1997 |
| Genre | : Medical |
| ISBN | : 9780879695323 |
Defines the current status of research in the genetics, anatomy, and development of the nematode C. elegans, providing a detailed molecular explanation of how development is regulated and how the nervous system specifies varied aspects of behavior. Contains sections on the genome, development, neural networks and behavior, and life history and evolution. Appendices offer genetic nomenclature, a list of laboratory strain and allele designations, skeleton genetic maps, a list of characterized genes, a table of neurotransmitter assignments for specific neurons, and information on codon usage. Includes bandw photos. For researchers in worm studies, as well as the wider community of researchers in cell and molecular biology. Annotation copyrighted by Book News, Inc., Portland, OR
| Author | : Carol Marie Garvin |
| Publisher | : |
| Total Pages | : 222 |
| Release | : 1996 |
| Genre | : |
| ISBN | : |
| Author | : Jason Burgess |
| Publisher | : |
| Total Pages | : 190 |
| Release | : 2002 |
| Genre | : Caenorhabditis elegans |
| ISBN | : |
"Mutations in the Caenorhabditis elegans maternal-effect gene clk-1 result in a highly pleiotropic phenotype, characterized by a general slow down in embryonic and larval development, as well as a slowing down of adult behaviors including defecation, pharyngeal pumping and swimming. First generation homozygous clk-1 mutants descended from a heterozygous mother are fully rescued for these mutant phenotypes. It has been shown that CLK-1 protein is a hydroxylase that acts in the conversion of demethoxyubiquinone (DMQ) to 5-hydroxyubiquinone, in the ubiquinone (Q) biosynthesis pathway. Consequently, clk-1 mutants accumulate the Q9 precursor, DMQ9 (the subscript refers to the length of the isoprenoid side chain). Here, I show that the profound maternal rescue observed in clk-1 maternally rescued animals is due to presence of the CLK-1 protein throughout larval development, in sufficient amounts to catalyze the production of Q9. clk-1 mutants have been shown to have a dietary requirement for Q8 due to their inability to synthesize Q9. I demonstrate that clk-1 maternally rescued animals have sufficient amounts of Q 9 to complete larval development and produce an almost full brood when raised on a Q8 deficient E. coli strain. I also show that prolonged arrest at the first larval stage, which is likely to result in degradation of any maternally contributed mRNA or protein, brings about a Clk mutant phenotype in maternally rescued animals. Finally, I reveal that the Clk mutant phenotype can be rescued at any larval stage by ectopic expression of CLK-1, suggesting that there is no developmental window for the rescue of clk-1 mutants by CLK-1. These results identify perdurance of maternally contributed product throughout development as the mechanism that accounts for the maternal effect observed in clk-1 mutants." --
| Author | : Ian A. Hope |
| Publisher | : OUP Oxford |
| Total Pages | : 306 |
| Release | : 1999-12-09 |
| Genre | : Science |
| ISBN | : 019159198X |
Caenorhabditis Elegans has been a popular model organism for biological research for over thirty years and has been used to investigate many aspects of animal development, for example apoptosis, the Hox genes, signal transduction pathways, and the development of the nervous system. It has recently taken on new importance with the publication of the entire genome sequence in 1998. The first chapter gives all the basic information on C. elegans required to use it: it's natural history, anatomy, life cycle, development, and evolution. Information on how to obtain, grow, and maintain C. elegans for use as a model system is given in Chapter 4. Chapters 2 and 3 describe the genome project and show how to use genome sequence information by searching the database for homologues using different search methods and then how to analyse the search data. The next chapter gives the essential practical details of transformation and common uses for the technique. Chapter 6 covers reverse genetics and describes strategies for gene inactivation that are known to work in C elegans: epigenetic inactivation and mutational germ line inactivation. Chapter 7 is designed to help the user analyse phenotype by microscopy and includes Normaski, fluorescence, 4-dimensional, and electron microscopy. Techniques for studying the neurobiology of C. elegans are given in chapter 8. Chapter 9 describes the three commonly used approaches for studying gene expression and Chapter 10 deals with the common methods of molecular biology essential for gene characterization. C. elegans is not the ideal organism for biochemical studies, but chapter 11 describes several procedures for producing biochemically useful quantities of pure tissues. The final chapter is about conventional genetics and details the standard procedures for selfing and crossing; mutagenesis and mutant screening; characterization of mutants; gene mapping; temperature-shift experiments and mosaic analysis. Caenorhabditis Elegans: A Practical Approach will therefore provide all the background information necessary for use of C. elegans as a model system.
