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A Beginner's Handbook in Biological Transmission Electron Microscopy

A Beginner's Handbook in Biological Transmission Electron Microscopy
Author: Brenda S. Weakley
Publisher:
Total Pages: 268
Release: 1981
Genre: Science
ISBN:

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Simplified basic theory of transmission electron microscopy; The processing of tissues; The preparation of ultrathin sections for electron microscopy; Staining of biological materials for electron microscopy; Routine operation of the electron microscope; Darkroom procedures; Some general suggestions for the study of ultrastructure; Ultrastructural cytochemistry; Autoradiography with the electron microscope; A brief synopsis of other techniques for the electron microscope; Routine maintenance of the electron microscope.


A Beginners' Guide to Scanning Electron Microscopy

A Beginners' Guide to Scanning Electron Microscopy
Author: Anwar Ul-Hamid
Publisher: Springer
Total Pages: 422
Release: 2018-10-26
Genre: Technology & Engineering
ISBN: 3319984829

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This book was developed with the goal of providing an easily understood text for those users of the scanning electron microscope (SEM) who have little or no background in the area. The SEM is routinely used to study the surface structure and chemistry of a wide range of biological and synthetic materials at the micrometer to nanometer scale. Ease-of-use, typically facile sample preparation, and straightforward image interpretation, combined with high resolution, high depth of field, and the ability to undertake microchemical and crystallographic analysis, has made scanning electron microscopy one of the most powerful and versatile techniques for characterization today. Indeed, the SEM is a vital tool for the characterization of nanostructured materials and the development of nanotechnology. However, its wide use by professionals with diverse technical backgrounds—including life science, materials science, engineering, forensics, mineralogy, etc., and in various sectors of government, industry, and academia—emphasizes the need for an introductory text providing the basics of effective SEM imaging.A Beginners’ Guide to Scanning Electron Microscopy explains instrumentation, operation, image interpretation and sample preparation in a wide ranging yet succinct and practical text, treating the essential theory of specimen-beam interaction and image formation in a manner that can be effortlessly comprehended by the novice SEM user. This book provides a concise and accessible introduction to the essentials of SEM includes a large number of illustrations specifically chosen to aid readers' understanding of key concepts highlights recent advances in instrumentation, imaging and sample preparation techniques offers examples drawn from a variety of applications that appeal to professionals from diverse backgrounds.


Biological Electron Microscopy

Biological Electron Microscopy
Author: Michael J. Dykstra
Publisher: Springer Science & Business Media
Total Pages: 368
Release: 2012-12-06
Genre: Science
ISBN: 146840010X

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In this practical text, the author covers the fundamentals of biological electron microscopy - including fixation, instrumentation, and darkroom work - to provide an excellent introduction to the subject for the advanced undergraduate or graduate student.


Practical Electron Microscopy

Practical Electron Microscopy
Author: Elaine Evelyn Hunter
Publisher: Cambridge University Press
Total Pages: 210
Release: 1993-09-24
Genre: Medical
ISBN: 9780521385398

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For this new edition, the chapters on photography and the electron microscope have been completely rewritten and two new chapters have been added--on immuno electron microscopy using colloidal gold and on useful specialized techniques.


Biological Specimen Preparation for Transmission Electron Microscopy

Biological Specimen Preparation for Transmission Electron Microscopy
Author: Audrey M. Glauert
Publisher: Princeton University Press
Total Pages: 349
Release: 2014-07-14
Genre: Science
ISBN: 1400865026

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This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen. The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements. All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs. Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, Editor Originally published in 1999. The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.


A Manual of Applied Techniques for Biological Electron Microscopy

A Manual of Applied Techniques for Biological Electron Microscopy
Author: Michael J. Dykstra
Publisher: Springer Science & Business Media
Total Pages: 280
Release: 1993-08-31
Genre: Medical
ISBN: 9780306444494

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This easy-to-follow manual describes tested procedures used to prepare biological samples for scanning and transmission electron microscopy, as well as methods for cytochemistry, immunocytochemistry, and scientific photography. The work is structured to clearly define testing objectives, necessary materials, procedural steps, and expected results; a list of references and trouble shooting techniques round out the text.


Advanced Techniques in Biological Electron Microscopy

Advanced Techniques in Biological Electron Microscopy
Author: J.K. Koehler
Publisher: Springer Science & Business Media
Total Pages: 319
Release: 2012-12-06
Genre: Science
ISBN: 3642654924

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The past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec tron optical equipment and interpretation of results is no longer restricted to a few prestigious centers. On the other hand, temniques utilized by researm workers in the ultrastructural domain have become extremely diverse and complex. Although a large number of quite excellent volumes of electron microscopic temnique are now dedicated to the basic elements available whim allow the novice to acquire a reasonable introduction to the field, relatively few books have been devoted to a discussion of more ad vanced temnical aspects of the art. It was with this view that the present volume was conceived as a handy reference for workers already having some background in the field, as an information source for those wishing to shift efforts into more promising temniques, or for use as an advanced course or seminar guide. Subject matter has been mosen particularly on the basis of pertinence to present researm activities in biological electron microscopy and emphasis has been given those areas whim seem destined to greatly expand in useful ness in the near future.