Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download The Purification And Characterization Of Orotidine 5 Monophosphate Decarboxylase From The Archaeon Sulfolobus Acidocaldarius PDF full book. Access full book title The Purification And Characterization Of Orotidine 5 Monophosphate Decarboxylase From The Archaeon Sulfolobus Acidocaldarius.
| Author | : John N. Maiorano |
| Publisher | : |
| Total Pages | : 162 |
| Release | : 1998 |
| Genre | : |
| ISBN | : |
| Author | : Michael P. Craig |
| Publisher | : |
| Total Pages | : 190 |
| Release | : 2001 |
| Genre | : |
| ISBN | : |
Abstract: Sequence, biochemical and structural analysis of the Sulfolobus acidocaldarius orotidine 5'-monophosphate decarboxylase was performed in order to identify and obtain an understanding of the structural features of the protein. Sequence analysis indicated that the enzyme has structural similarity to the mesophilic counterparts, all of which have an ([alpha], [beta]) -TIM barrel structure. The enzyme exhibited a V max with an upper limit of approximately 0.205 +/- 0.013 æmol UMP formed per min per mg protein, and a K m at pH8.5 under 1.0uM. The enzyme yielded activity half-lives of 62, 44, 9, 5, and 2 min at 70, 80, 85, 90, and 95ʻC respectively. The recombinant enzyme was stabilized by excess product (UMP), a fact supported by activity measurements UV spectra. Direct assay for thiols by SDS-PAGE, using SBF-Cl detection, confirmed the presence of a pair of disulfide linkages in the active dimer. Treatment with low-level denaturants resulted in no elevation of activity at lower temperatures (as observed with other thermophilic proteins). Attempts in trapping a folding intermediate using cold renaturation experiments failed, indicating that guanidine-induced unfolding is completely reversible and occurs readily even at low temperature. Preparation of a gene encoding a cys-less mutant ODC is also described.
| Author | : Edward F. DeLong |
| Publisher | : Springer |
| Total Pages | : 567 |
| Release | : 2014-10-13 |
| Genre | : Science |
| ISBN | : 9783642301193 |
The Prokaryotes is a comprehensive, multi-authored, peer reviewed reference work on Bacteria and Achaea. This fourth edition of The Prokaryotes is organized to cover all taxonomic diversity, using the family level to delineate chapters. Different from other resources, this new Springer product includes not only taxonomy, but also prokaryotic biology and technology of taxa in a broad context. Technological aspects highlight the usefulness of prokaryotes in processes and products, including biocontrol agents and as genetics tools. The content of the expanded fourth edition is divided into two parts: Part 1 contains review chapters dealing with the most important general concepts in molecular, applied and general prokaryote biology; Part 2 describes the known properties of specific taxonomic groups. Two completely new sections have been added to Part 1: bacterial communities and human bacteriology. The bacterial communities section reflects the growing realization that studies on pure cultures of bacteria have led to an incomplete picture of the microbial world for two fundamental reasons: the vast majority of bacteria in soil, water and associated with biological tissues are currently not culturable, and that an understanding of microbial ecology requires knowledge on how different bacterial species interact with each other in their natural environment. The new section on human microbiology deals with bacteria associated with healthy humans and bacterial pathogenesis. Each of the major human diseases caused by bacteria is reviewed, from identifying the pathogens by classical clinical and non-culturing techniques to the biochemical mechanisms of the disease process. The 4th edition of The Prokaryotes is the most complete resource on the biology of prokaryotes. The following volumes are published consecutively within the 4th Edition: Prokaryotic Biology and Symbiotic Associations Prokaryotic Communities and Ecophysiology Prokaryotic Physiology and Biochemistry Applied Bacteriology and Biotechnology Human Microbiology Actinobacteria Firmicutes Alphaproteobacteria and Betaproteobacteria Gammaproteobacteria Deltaproteobacteria and Epsilonproteobacteria Other Major Lineages of Bacteria and the Archaea
| Author | : Byung Hong Kim |
| Publisher | : Cambridge University Press |
| Total Pages | : 934 |
| Release | : 2008-02-21 |
| Genre | : Science |
| ISBN | : 113946762X |
Recent determination of genome sequences for a wide range of bacteria has made in-depth knowledge of prokaryotic metabolic function essential in order to give biochemical, physiological, and ecological meaning to the genomic information. Clearly describing the important metabolic processes that occur in prokaryotes under different conditions and in different environments, this advanced text provides an overview of the key cellular processes that determine bacterial roles in the environment, biotechnology, and human health. Prokaryotic structure is described as well as the means by which nutrients are transported into cells across membranes. Glucose metabolism through glycolysis and the TCA cycle are discussed, as well as other trophic variations found in prokaryotes, including the use of organic compounds, anaerobic fermentation, anaerobic respiratory processes, and photosynthesis. The regulation of metabolism through control of gene expression and control of the activity of enzymes is also covered, as well as survival mechanisms used under starvation conditions.
| Author | : Richa Salwan |
| Publisher | : Academic Press |
| Total Pages | : 388 |
| Release | : 2020-06-04 |
| Genre | : Science |
| ISBN | : 0128183233 |
Physiological and Biotechnological Aspects of Extremophiles highlights the current and topical areas of research in this rapidly growing field. Expert authors from around the world provide the latest insights into the mechanisms of these fascinating organisms use to survive.The vast majority of extremophiles are microbes which include archaea, bacteria and some eukaryotes. These microbes live under chemical and physical extremes that are usually lethal to cellular molecules, yet they manage to survive and even thrive. Extremophiles have important practical uses. They are a valuable source of industrially important enzymes and recent research has revealed novel mechanisms and biomolecular structures with a broad range of potential applications in biotechnology, biomining, and bioremediation.Aimed at research scientists, students, microbiologists, and biotechnologists, this book is an essential reading for scientists working with extremophiles and a recommended reference text for anyone interested in the microbiology, bioprospecting, biomining, biofuels, and extremozymes of these organisms. Shows the implications of the physiological adaptations of microbes from extreme habitats that are largely contributed by their biomolecules from basic to applied research Provides in-depth knowledge of genomic plasticity and proteome of different extremophiles Gives detailed and comprehensive insight about use of genetic engineering as well as genome editing for industrial applications
| Author | : Tessa Quax |
| Publisher | : |
| Total Pages | : 152 |
| Release | : 2013 |
| Genre | : |
| ISBN | : 9789461737830 |
The work presented in this thesis provides novel insights in several aspects of the molecular biology of Archaea, Bacteria and their viruses. The archaeal virus Sulfolobus islandicus rod-shaped virus 2 (SIRV2), has a remarkable infection cycle. Infection with SIRV2 results in the formation of large virus associated pyramids (VAPs) on the host cell surface. The pyramids open during the final step of the infection cycle, to allow the release of virions. This virus release mechanism is unique. The VAPs are formed by self-assembly of one virus-encoded protein, PVAP. VAPs exist as discrete particles, and are baseless pyramids with heptagonal perimeter. The assembly process of the VAPs is described, based on cryo-electron tomography experiments and mutational analysis of PVAP. VAPs consists of two layers of which the outer one continuous with the cell membrane. PVAP expression in bacterial and eukaryotic cells resulted in VAP formation on nearly all membranes, demonstrating that PVAP serves as a universal membrane remodeling system, which might be exploited for biotechnological purposes. Whole transcriptome sequencing allowed determination of a global map of virus and host gene expression during the infection cycle. Host genes involved in anti-viral defence are activated (i.e. CRISPR-Cas and toxin anti-toxin systems). The multi-subunit protein complexes crucial for CRISPR anti-viral defence have an uneven stoichiometry and are encoded on operons. It is shown that differential translation is a key determinant of modulated expression of genes clustered in operons and that codon bias generally is the best in silico indicator of unequal protein production.
| Author | : Stanley Falkow |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 1157 |
| Release | : 2006-07-13 |
| Genre | : Science |
| ISBN | : 0387254927 |
The revised Third Edition of The Prokaryotes, acclaimed as a classic reference in the field, offers new and updated articles by experts from around the world on taxa of relevance to medicine, ecology and industry. Entries combine phylogenetic and systematic data with insights into genetics, physiology and application. Existing entries have been revised to incorporate rapid progress and technological innovation. The new edition improves on the lucid presentation, logical layout and abundance of illustrations that readers rely on, adding color illustration throughout. Expanded to seven volumes in its print form, the new edition adds a new, searchable online version.
| Author | : Michael A. Innis |
| Publisher | : Elsevier |
| Total Pages | : 391 |
| Release | : 1995-07-06 |
| Genre | : Science |
| ISBN | : 0080538541 |
PCR Strategies expands and updates the landmark volume PCR Protocols. It is a companion laboratory manual that provides a completely new set of up-to-date strategies and protocols for getting the most from PCR. The editors have organized the book into four sections, focusing on principles, analyses, research applications, and alternative strategies for a wide variety of basic and clinical needs. If you own PCR Protocols, you will want PCR Strategies. If you don't own PCR Protocols, you will want to buy both! Concepts explained Methods detailed Trouble-shooting emphasized Novel applications highlighted Key concepts for PCR Analysis of PCR products Research applications Alternative amplification strategies
| Author | : Susan Hummel |
| Publisher | : CreateSpace |
| Total Pages | : 86 |
| Release | : 2015-02-14 |
| Genre | : |
| ISBN | : 9781506088112 |
Beargrass is a source of food, habitat, and raw material for animals, pollinating insects and people across its range in the Western United States. The plant has long been used by Native Americans, who harvest the leaves for basketry and other crafts. More recently, beargrass has become an important component of international trade for the commercial floral greens industry.
| Author | : |
| Publisher | : Academic Press |
| Total Pages | : 0 |
| Release | : 2017-09-01 |
| Genre | : Medical |
| ISBN | : 9780128119440 |
Fe-S Cluster Enzymes, Part A, Volume 595 is the first of two volumes focused on Fe-S cluster enzymes. New topics of note in this series include Electrochemistry of Fe/S Proteins, Genetic, biochemical and biophysical methods for studying Fe-S proteins and their assembly, Fluorescent reporters to track Fe-S cluster assembly and transfer reactions, Mechanism-based strategies for structural characterization of radical SAM reaction intermediates, Purification and Characterization of Recalcitrant Cobalamin-Dependent Radical S-adenosylmethionine Methylases, A polymerase with potential: the Fe-S cluster in Human DNA Primase, In Vitro Studies of Cellular Iron-sulfur Cluster Biosynthesis, Trafficking and Transport, and Fe-S cluster Hsp70 Chaperones: the ATPase cycle and protein interactions.
