Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download The Applicability Of Whole Genome Amplification On Minute Samples Of Dna For Forensic Use PDF full book. Access full book title The Applicability Of Whole Genome Amplification On Minute Samples Of Dna For Forensic Use.
| Author | : Barbara L. Leal |
| Publisher | : |
| Total Pages | : 120 |
| Release | : 2004 |
| Genre | : |
| ISBN | : |
| Author | : Amy Leigh Barber |
| Publisher | : |
| Total Pages | : 142 |
| Release | : 2005 |
| Genre | : DNA fingerprinting |
| ISBN | : |
| Author | : E. K. Wheeler |
| Publisher | : |
| Total Pages | : 7 |
| Release | : 2005 |
| Genre | : |
| ISBN | : |
Many challenges arise when trying to amplify and analyze human samples collected in the field due to limitations in sample quantity, and contamination of the starting material. Tests such as DNA fingerprinting and mitochondrial typing require a certain sample size and are carried out in large volume reactions; in cases where insufficient sample is present whole genome amplification (WGA) can be used. WGA allows very small quantities of DNA to be amplified in a way that enables subsequent DNA-based tests to be performed. A limiting step to WGA is sample preparation. To minimize the necessary sample size, we have developed two modifications of WGA: the first allows for an increase in amplified product from small, nanoscale, purified samples with the use of carrier DNA while the second is a single-step method for cleaning and amplifying samples all in one column. Conventional DNA cleanup involves binding the DNA to silica, washing away impurities, and then releasing the DNA for subsequent testing. We have eliminated losses associated with incomplete sample release, thereby decreasing the required amount of starting template for DNA testing. Both techniques address the limitations of sample size by providing ample copies of genomic samples. Carrier DNA, included in our WGA reactions, can be used when amplifying samples with the standard purification method, or can be used in conjunction with our single-step DNA purification technique to potentially further decrease the amount of starting sample necessary for future forensic DNA-based assays.
| Author | : Megan Frances Palmer |
| Publisher | : |
| Total Pages | : 180 |
| Release | : 2011 |
| Genre | : DNA |
| ISBN | : |
| Author | : Glenn M. G. Theunissen |
| Publisher | : |
| Total Pages | : |
| Release | : 2020 |
| Genre | : |
| ISBN | : |
| Author | : National Research Council |
| Publisher | : National Academies Press |
| Total Pages | : 270 |
| Release | : 1996-12-12 |
| Genre | : Science |
| ISBN | : 0309134404 |
In 1992 the National Research Council issued DNA Technology in Forensic Science, a book that documented the state of the art in this emerging field. Recently, this volume was brought to worldwide attention in the murder trial of celebrity O. J. Simpson. The Evaluation of Forensic DNA Evidence reports on developments in population genetics and statistics since the original volume was published. The committee comments on statements in the original book that proved controversial or that have been misapplied in the courts. This volume offers recommendations for handling DNA samples, performing calculations, and other aspects of using DNA as a forensic toolâ€"modifying some recommendations presented in the 1992 volume. The update addresses two major areas: Determination of DNA profiles. The committee considers how laboratory errors (particularly false matches) can arise, how errors might be reduced, and how to take into account the fact that the error rate can never be reduced to zero. Interpretation of a finding that the DNA profile of a suspect or victim matches the evidence DNA. The committee addresses controversies in population genetics, exploring the problems that arise from the mixture of groups and subgroups in the American population and how this substructure can be accounted for in calculating frequencies. This volume examines statistical issues in interpreting frequencies as probabilities, including adjustments when a suspect is found through a database search. The committee includes a detailed discussion of what its recommendations would mean in the courtroom, with numerous case citations. By resolving several remaining issues in the evaluation of this increasingly important area of forensic evidence, this technical update will be important to forensic scientists and population geneticistsâ€"and helpful to attorneys, judges, and others who need to understand DNA and the law. Anyone working in laboratories and in the courts or anyone studying this issue should own this book.
| Author | : National Research Council |
| Publisher | : National Academies Press |
| Total Pages | : 199 |
| Release | : 1992-02-01 |
| Genre | : Science |
| ISBN | : 0309045878 |
Matching DNA samples from crime scenes and suspects is rapidly becoming a key source of evidence for use in our justice system. DNA Technology in Forensic Science offers recommendations for resolving crucial questions that are emerging as DNA typing becomes more widespread. The volume addresses key issues: Quality and reliability in DNA typing, including the introduction of new technologies, problems of standardization, and approaches to certification. DNA typing in the courtroom, including issues of population genetics, levels of understanding among judges and juries, and admissibility. Societal issues, such as privacy of DNA data, storage of samples and data, and the rights of defendants to quality testing technology. Combining this original volume with the new update-The Evaluation of Forensic DNA Evidence-provides the complete, up-to-date picture of this highly important and visible topic. This volume offers important guidance to anyone working with this emerging law enforcement tool: policymakers, specialists in criminal law, forensic scientists, geneticists, researchers, faculty, and students.
| Author | : Ralph Rapley |
| Publisher | : John Wiley & Sons |
| Total Pages | : 33 |
| Release | : 2007-05-21 |
| Genre | : Science |
| ISBN | : 047002495X |
Molecular Forensics offers a comprehensive coverage of the increasingly important role that molecular analysis plays within forensic science. Starting with a broad introduction of modern forensic molecular technologies, the text covers key issues from the initial scenes of crime sampling to the use of evidential material in the prosecution of legal cases. The book also explores the questions raised by the growing debate on the applications of national DNA databases and the resulting challenges of developing, maintaining and curating such vast data structures. The broader range of applications to non-human cases is also discussed, as are the statistical pitfalls of using so-called unique data such as DNA profiles, and the ethical considerations of national DNA databases. An invaluable reference for students taking courses within the Forensic and Biomedical sciences, and also useful for practitioners in the field looking for a broad overview of the subject. Provides a comprehensive overview of modern forensic molecular technologies. Explores the growing debate on the applications of national DNA databases. Discusses the initial phases of investigation to the conclusion of cases involving molecular forensic analysis.
| Author | : Simon Hughes |
| Publisher | : Methods Express (Paperback) |
| Total Pages | : 224 |
| Release | : 2005 |
| Genre | : Medical |
| ISBN | : |
Whole genome amplification generates microgram quantities of genomic DNA starting from a sample of as little as a few femtograms and so is a vital technique when sample material is limited, as well as for high-throughput assays. Whole Genome Amplification: Methods Expressis a comprehensive up-to-date laboratory manual for this key technique. It provides detailed step-by-step protocols as well as hints and tips for success and troubleshooting, taking readers through all aspects of whole genome amplification. This book is an essential practical guide for any researcher currently using PCR for genomic amplification or who wishes to do so in future.
| Author | : Bruce Budowle |
| Publisher | : Academic Press |
| Total Pages | : 753 |
| Release | : 2010-10-27 |
| Genre | : Science |
| ISBN | : 0123820073 |
Microbial Forensics is a rapidly evolving scientific discipline. In the last decade, and particularly due to the anthrax letter attacks in the United States, microbial forensics has become more formalized and has played an increasingly greater role in crime investigations. This has brought renewed interest, development and application of new technologies, and new rules of forensic and policy engagement. It has many applications ranging from biodefense, criminal investigations, providing intelligence information, making society more secure, and helping protect precious resources, particularly human life. A combination of diverse areas is investigated, including the major disciplines of biology, microbiology, medicine, chemistry, physics, statistics, population genetics, and computer science. Microbial Forensics, Second Edition is fully revised and updated and serves as a complete reference of the discipline. It describes the advances, as well as the challenges and opportunities ahead, and will be integral in applying science to help solve future biocrimes. A collection of microbiology, virology, toxicology and mycology as it relates to forensics, in one reference New and expanded content to include statistical analysis of forensic data and legal admissibility and the standards of evidence, to name a few Includes research information and application of that research to crime scene analysis, which will allow practitioners to understand and apply the knowledge to their practice with ease
