Test No 455 Performance Based Test Guideline For Stably Transfected Transactivation In Vitro Assays To Detect Estrogen Receptor Agonists PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Test No 455 Performance Based Test Guideline For Stably Transfected Transactivation In Vitro Assays To Detect Estrogen Receptor Agonists PDF full book. Access full book title Test No 455 Performance Based Test Guideline For Stably Transfected Transactivation In Vitro Assays To Detect Estrogen Receptor Agonists.
Author | : OECD |
Publisher | : OECD Publishing |
Total Pages | : 39 |
Release | : 2012-10-02 |
Genre | : |
ISBN | : 9264185380 |
Download Test No. 455: Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists Book in PDF, ePub and Kindle
This Performance-Based Test Guideline (PBTG) describes in vitro assays, which provides the methodology of Stably Transfected Transactivation to detect Estrogen Receptor Agonists (ER TAs). It comprises mechanistically and functionally similar test ...
Author | : OECD |
Publisher | : OECD Publishing |
Total Pages | : 53 |
Release | : 2015-07-28 |
Genre | : |
ISBN | : 9264243046 |
Download Test No. 455: Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists and Antagonists Book in PDF, ePub and Kindle
This Performance-Based Test Guideline (PBTG) describes in vitro assays, which provide the methodology of Stably Transfected Transactivation to detect Estrogen Receptor Agonists and Antagonists (ER TA assays).
Author | : OECD |
Publisher | : OECD Publishing |
Total Pages | : 74 |
Release | : 2021-06-17 |
Genre | : |
ISBN | : 9264265295 |
Download OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 455: Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists and Antagonists Book in PDF, ePub and Kindle
This Performance-Based Test Guideline (PBTG) describes in vitro assays, which provide the methodology of Stably Transfected Transactivation to detect Estrogen Receptor Agonists and Antagonists (ER TA assays).
Author | : |
Publisher | : |
Total Pages | : 54 |
Release | : 2015 |
Genre | : |
ISBN | : |
Download Test No. 455: Draft Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists and Antagonists Book in PDF, ePub and Kindle
This Performance-Based Test Guideline (PBTG) describes in vitro assays, which provide the methodology of Stably Transfected Transactivation to detect Estrogen Receptor Agonists and Antagonists (ER TA assays). It comprises mechanistically and functionally similar test methods for the identification of estrogen receptor agonists and antagonists and should facilitate the development of new similar or modified test methods. The two reference test methods that provide the basis for this PBTG are: the Stably Transfected TA (STTA) assay using the (h) ERÎł-HeLa-9903 cell line, derived from a human cervical tumor, and the BG1Luc ER TA assay using the BG1Luc-4E2 cell line, derived from a human ovarian adenocarcinoma. The cell lines used in these assays express ER and have been stably transfected with an ER responsive luciferase reporter gene. The assays are used to identify chemicals that activate (id est act as agonists) and also suppress (id est act as antagonists) ER- dependent transcription. ER are activated following ligand binding, after which the receptor-ligand complex binds to specific DNA response elements and transactivates the reporter gene, resulting in increased cellular expression of a marker enzyme (e.g. luciferase in luciferase based systems). The enzyme then transforms the substrate to a bioluminescent product that can be quantitatively measured with a luminometer. These test methods are being proposed for screening and prioritisation purposes, but also provide mechanistic information that can be used in a weight of evidence approach.
Author | : Organisation de coopération et de développement économiques |
Publisher | : |
Total Pages | : 54 |
Release | : 2015 |
Genre | : |
ISBN | : |
Download Test No. 455: Draft Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists and Antagonists Book in PDF, ePub and Kindle
Author | : Frank Dreher |
Publisher | : CRC Press |
Total Pages | : 997 |
Release | : 2022-08-11 |
Genre | : Medical |
ISBN | : 1000590623 |
Download Handbook of Cosmetic Science and Technology Book in PDF, ePub and Kindle
With chapters from experienced and internationally renowned contributors holding positions in research, industry, and clinical practice, this is the fifth edition of what has become the standard reference for cosmetic scientists and dermatologists seeking the latest innovations and technology for the formulation, design, testing, use, and production of cosmetic products for the skin. *Offers in-depth analysis of specific topics in cosmetic science and research *Presents the latest in international research and its translation to practice *Gives an indispensable guide to a hotly competitive area for research and practice
Author | : Harikrishnan Vijayakumar Sreelatha |
Publisher | : Springer Nature |
Total Pages | : 645 |
Release | : |
Genre | : |
ISBN | : 9819700485 |
Download Animal Models in Research Book in PDF, ePub and Kindle
Author | : Chantra Eskes |
Publisher | : Springer |
Total Pages | : 418 |
Release | : 2016-09-26 |
Genre | : Medical |
ISBN | : 3319338269 |
Download Validation of Alternative Methods for Toxicity Testing Book in PDF, ePub and Kindle
This book provides information on best practices and new thinking regarding the validation of alternative methods for toxicity testing. It covers the validation of experimental and computational methods and integrated approaches to testing and assessment. Validation strategies are discussed for methods employing the latest technologies such as tissue-on-a-chip systems, stem cells and transcriptomics, and for methods derived from pathway-based concepts in toxicology. Validation of Alternative Methods for Toxicity Testing is divided into two sections, in the first, practical insights are given on the state-of-the-art and on approaches that have resulted in successfully validated and accepted alternative methods. The second section focuses on the evolution of validation principles and practice that are necessary to ensure fit-for-purpose validation that has the greatest impact on international regulatory acceptance of alternative methods. In this context validation needs to keep pace with the considerable scientific advancements being made in toxicology, the availability of sophisticated tools and techniques that can be applied in a variety of ways, and the increasing societal and regulatory demands for better safety assessment. This book will be a useful resource for scientists in the field of toxicology, both from industry and academia, developing new test methods, strategies or techniques, as well as Governmental and regulatory authorities interested in understanding the principles and practicalities of validation of alternative methods for toxicity testing.
Author | : J.P.F. D'Mello |
Publisher | : CABI |
Total Pages | : 675 |
Release | : 2019-12-04 |
Genre | : Science |
ISBN | : 1786394677 |
Download A Handbook of Environmental Toxicology Book in PDF, ePub and Kindle
Written by an international team of authors from a range of educational, medical and research establishments, this book is an essential reference for advanced students and researchers in the areas of environmental sciences, ecology, agriculture, environmental health and medicine, in addition to industry and government personnel responsible for environmental regulations and directives. A Handbook of Environmental Toxicology focuses on two key aspects: human disorders and ecotoxicology as affected by major toxins originating from biological sources and pollutants, as well as radiation generated spontaneously or as a result of anthropogenic activity. A diverse array of these potentially harmful agents regularly appear in the atmosphere, soil, water and food, compromising both human health and biodiversity in natural and managed ecosystems.
Author | : OECD |
Publisher | : OECD Publishing |
Total Pages | : 45 |
Release | : 2021-06-17 |
Genre | : |
ISBN | : 9264886109 |
Download OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 250: EASZY assay - Detection of Endocrine Active Substances, acting through estrogen receptors, using transgenic tg(cyp19a1b:GFP) Zebrafish embrYos Book in PDF, ePub and Kindle
The EASZY assay is a mechanism-based in vivo screening assay designed to detect endocrine active chemicals acting as agonist through estrogen receptors (ERs), by inducing the expression of the green fluorescent protein (GFP) driven by the cyp19a1b promoter. The EASZY assay allows for the detection of estrogenic activity of chemicals on transgenic tg(cyp19a1b:GFP) Zebrafish embryos exposed for 96 hours during the embryonic stages of development.