Techniques In Protein Modification PDF Download
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Author | : Roger L. Lundblad |
Publisher | : CRC Press |
Total Pages | : 300 |
Release | : 1994-11-17 |
Genre | : Medical |
ISBN | : 9780849326066 |
Download Techniques in Protein Modification Book in PDF, ePub and Kindle
This exciting laboratory support book encompasses the encyclopedic coverage of the most frequently used methods for the site-specific chemical modification of proteins. In a concise manner, it presents methods for the characterization of modified proteins, including: amino acid analysis protein sequence analysis chemical cleavage of protein chains chromatographic separation of peptides It also discusses various approaches to the determination of solution protein concentration. It includes a complete literature survey of the various reagents, a list of the most commonly used reagents with their physical and chemical properties, and a list of preferred reagent suppliers.
Author | : Roger L. Lundblad |
Publisher | : CRC Press |
Total Pages | : 352 |
Release | : 2020-03-11 |
Genre | : Medical |
ISBN | : 1000694712 |
Download Chemical Reagents for Protein Modification Book in PDF, ePub and Kindle
First published in 1991, Chemical Reagents for Protein Modification, 2nd Edition provides a unique combination of theoretical and practical considerations for the use of chemical reagents for site-specific modification of proteins. The book is divided into three sections, with the first section describing general techniques, including information on the organic chemistry of the various modification reactions; the separation and characterization of site-specific modified proteins, including applications to proteins separated by electrophoresis followed by blotting; the specific chemical cleavage of peptide bonds in proteins; the separation of peptides by high-performance liquid chromatography and electrophoresis; and the use of chemical reagents to assess conformational change in proteins. The second section provides an encyclopedic description of reagents and reactions for the site-specific modification of individual amino acid residues in proteins. The final section presents descriptions of the use of chemical reagents to label biologically significant sites in proteins, including enzyme active sites and the use of covalent cross-linking to measure protein-protein interactions. Particular emphasis is placed on the use of photoaffinity reagents. The book will be an extremely useful research tool for all investigators interested in the solution chemistry of proteins.
Author | : Oscar Alzate |
Publisher | : CRC Press |
Total Pages | : 356 |
Release | : 2009-10-26 |
Genre | : Medical |
ISBN | : 1420076264 |
Download Neuroproteomics Book in PDF, ePub and Kindle
In this, the post-genomic age, our knowledge of biological systems continues to expand and progress. As the research becomes more focused, so too does the data. Genomic research progresses to proteomics and brings us to a deeper understanding of the behavior and function of protein clusters. And now proteomics gives way to neuroproteomics as we beg
Author | : Roger L. Lundblad |
Publisher | : Springer Science & Business |
Total Pages | : 360 |
Release | : 2005 |
Genre | : Medical |
ISBN | : 9780849319839 |
Download Chemical Reagents for Protein Modification, Third Edition Book in PDF, ePub and Kindle
Revised and updated, Chemical Reagents for Protein Modification, Third Edition is an encyclopedic work describing the many approaches to the site-specific modification of proteins. More than 2,000 references are cited describing the development of the various reagents. This book explores how the use of site-specific protein modification relates to a wide variety of biotechnology processes and products, such as protein microarrays, hydrogels, controlled-release pharmaceuticals, and biotherapeutics, and proteomics. With chapters focusing on analyses of individual amino acids, additional chapters cover chemical cross-linking and the chemical cleavage of peptide chains. There is considerable reference to the use of site-specific chemical modification in proteomics. The author pays particular attention to the general environmental factors that influence the reactivity of individual amino acid residues. He presents techniques for the characterization of modified proteins, including mass spectrometry, and includes increased coverage of spectroscopic techniques and crystallographic analyses. This is followed by coverage of nitrosylation reactions and other non-enzymatic modification processes. Specific reference is given to the differential labeling of protein functional groups with reagents labeled with stable isotopes. Resources include a list of current journals that publish site-specific chemical modification studies, the most commonly used reagents and their properties, and a contact list of reagent suppliers. Chemical Reagents for Protein Modification, 3rd Edition presents the most frequently used methods for the site-specific chemical modification of proteins, techniques for protein characterization, precise laboratory data for factors that influence reactivity and reproducibility, and industry-specific resources.
Author | : Roger L. Lundblad |
Publisher | : CRC Press |
Total Pages | : 678 |
Release | : 2014-07-22 |
Genre | : Medical |
ISBN | : 1466571918 |
Download Chemical Reagents for Protein Modification Book in PDF, ePub and Kindle
The use of the chemical modification of proteins has evolved over the past 80 years, benefiting from advances in analytical, physical, and organic chemistry. Over the past 30 years, the use of chemical reagents to modify proteins has been crucial in determining the function and structure of purified proteins. This groundbreaking work is part of the
Author | : Tanveer Ali Dar |
Publisher | : Academic Press |
Total Pages | : 0 |
Release | : 2019-05-21 |
Genre | : Science |
ISBN | : 9780128119136 |
Download Protein Modificomics Book in PDF, ePub and Kindle
Protein Modificomics: From Modifications to Clinical Perspectives comprehensively deals with all of the most recent aspects of post-translational modification (PTM) of proteins, including discussions on diseases involving PTMs, such as Alzheimer's, Huntington's, X-linked spinal muscular atrophy-2, aneurysmal bone cyst, angelman syndrome and OFC10. The book also discusses the role PTMs play in plant physiology and the production of medicinally important primary and secondary metabolites. The understanding of PTMs in plants helps us enhance the production of these metabolites without greatly altering the genome, providing robust eukaryotic systems for the production and isolation of desired products without considerable downstream and isolation processes.
Author | : Harald Tschesche |
Publisher | : Walter de Gruyter |
Total Pages | : 379 |
Release | : 2011-12-23 |
Genre | : Science |
ISBN | : 3110252368 |
Download Methods in Protein Biochemistry Book in PDF, ePub and Kindle
This book presents a survey of recent developments in protein biochemistry. Top researchers in the field of protein biochemistry describe modern methods to address the challenges of protein purification by three-phase partitioning, and their folding and degradation by the functions of chaperones. The significance of peptide purity for fibril formation is addressed as well as the use of target oriented peptide arrays in palliative approaches in mucoviszidose. The design and application of protein epitope mimetics just as the structural resolving of the misfolding of various mutant proteins in serpinopathies enlarge our tools in resolving pathophysiological imbalances.
Author | : Roger L. Lundblad |
Publisher | : Springer |
Total Pages | : 200 |
Release | : 1984-04-18 |
Genre | : Science |
ISBN | : |
Download CHEMICAL REAGENTS FOR PROTEIN MODIFICATION Book in PDF, ePub and Kindle
The chemical modification of proteins; Amino acid analysis; Peptide separation by reverse-phase high performance liquid chromatography; Methods for sequence determination; Chemical cleavage of peptide bonds; The modification of cystine; The Modification of cystine - cleavage of disulfide bonds; The modification of methionine; Modification of histidine residues; The modification of Lysine.
Author | : |
Publisher | : Elsevier |
Total Pages | : 501 |
Release | : 1976-12-01 |
Genre | : Science |
ISBN | : 0080858732 |
Download Chemical Modification of Proteins Book in PDF, ePub and Kindle
Chemical Modification of Proteins
Author | : Roger L. Lundblad |
Publisher | : Springer |
Total Pages | : 194 |
Release | : 1984-04-18 |
Genre | : Medical |
ISBN | : |
Download CHEMICAL REAGENTS FOR PROTEIN MODIFICATION Book in PDF, ePub and Kindle
The chemical modification of proteins; Amino acid analysis; Peptide separation by reverse-phase high performance liquid chromatography; Methods for sequence determination; Chemical cleavage of peptide bonds; The modification of cystine; The Modification of cystine - cleavage of disulfide bonds; The modification of methionine; Modification of histidine residues; The modification of Lysine.