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RNA Biochemistry and Biotechnology

RNA Biochemistry and Biotechnology
Author: Jan Barciszewski
Publisher: Springer Science & Business Media
Total Pages: 373
Release: 2012-12-06
Genre: Science
ISBN: 9401144850

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RNA Biochemistry and Biotechnology describes various aspects of nucleic acid and protein structure, mainly RNA structure and proteins, interacting with specific RNA species. Papers deal with DNA protein interactions, telomerase, aminoacyl-tRNA synthetases, elongation factor Tu, DNA repair, RNA structure, NMR technology, RNA aptamer interaction of biological macromolecules with metal ions. Two papers deal with theoretical aspects of RNA structure production and computer modelling. Many papers describe the possibility of commercial application of RNA biotechnology. One article discusses the impact of direct democracy on basic science supporting biotechnology. Readership: Advanced graduate students, Ph.D. students and young scientists as well as specialists in the field.


Enzymes in RNA Science and Biotechnology Part B

Enzymes in RNA Science and Biotechnology Part B
Author:
Publisher: Elsevier
Total Pages: 348
Release: 2023-11-02
Genre: Science
ISBN: 0443239770

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Enzymes in RNA Science and Biotechnology, Part B, Volume 692 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as Quantitative base-resolution sequencing technology for mapping pseudouridines in mammalian mRNA, Quantitative base-resolution DAMM-seq for mapping RNA methylations in tRNA and mitochondrial dsRNA, Discovering RNA modification enzymes using a comparative genomics approach, Functional analysis of tRNA modification enzymes using mutational profiling, and Fluorescent labeling of tRNA for rapid kinetic interaction studies with tRNA-binding proteins. Other chapters cover Enzymatic Synthesis of RNA Standards for Mapping and Quantifying RNA Modifications in Sequencing Analysis, Characterizing RNase L-mediated mRNA decay in single cells, Characterization of RNase J, Pri-miRNA cleavage assays for the Microprocessor complex, The pre-miRNA cleavage assays for DICE, Methods for study of ribonuclease targeting chimeras, and Profiling the in vivo RNA interactome associated with the endoribonuclease RNase III in Staphylococcus aureus. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in Methods in Enzymology series Updated release includes the latest information on Enzymes in RNA Science and Biotechnology


Analysis of RNA-Protein Complexes in vitro

Analysis of RNA-Protein Complexes in vitro
Author: J. Kjems
Publisher: Elsevier
Total Pages: 249
Release: 1998-11-13
Genre: Science
ISBN: 0080886965

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The central role of RNA in many cellular processes, in biotechnology, and as pharmaceutical agents, has created an interest in experimental methods applied to RNA molecules. This book provides scientists with a comprehensive collection of thoroughly tested up-to-date manuals for investigating RNA-protein complexes in vitro. The protocols can be performed by researchers trained in standard molecular biological techniques and require a minimum of specialized equipment. The procedures include recommendation of suppliers of reagents.


RNA Nanotechnology

RNA Nanotechnology
Author: Bin Wang
Publisher: CRC Press
Total Pages: 452
Release: 2014-04-02
Genre: Medical
ISBN: 9814411655

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In the past few decades there has been incredible growth in "bionano"-related research, which has been accompanied by numerous publications in this field. Although various compilations address topics related to deoxyribonucleic acid (DNA) and protein, there are few books that focus on determining the structure of ribonucleic acid (RNA) and using RN


Handbook of RNA Biochemistry

Handbook of RNA Biochemistry
Author: Boston Carter
Publisher:
Total Pages: 0
Release: 2023-09-19
Genre: Science
ISBN: 9781647403638

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Non-coding RNAs (ncRNAs) are RNA molecules which are not translated into proteins. They are classified into two types based on their length, including short non-coding RNAs comprising microRNAs (miRNAs) and long non-coding RNAs (lncRNAs). They play a role in gene regulation at various levels, ranging from epigenetic gene silencing to post-transcriptional modulation of mRNA stability. The ncRNAs contribute in the regulation of cell physiology and shape cellular functions. They play an important role in brain development and function, and are also associated with the pathogenesis of psychiatric disorders. Several ncRNAs including circRNAs, miRNAs and lncRNAs have been implicated in regulatory functions. Furthermore, they are believed to play a role in Alzheimer's disease and cancer. This book explores all the important aspects of the biochemistry of RNA. It presents researches and studies performed by experts across the globe. The extensive content of this book provides the readers with a thorough understanding of this topic.


Molecular Biology of RNA

Molecular Biology of RNA
Author: David Elliott
Publisher: Oxford University Press
Total Pages: 437
Release: 2016
Genre: Medical
ISBN: 0199671397

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RNA plays a central, and until recently, somewhat underestimated role in the genetics underlying all forms of life on earth. This versatile molecule not only plays a crucial part in the synthesis of proteins from a DNA template, but is also intrinsically involved in the regulation of gene expression, and in catalysis.This landmark text provides a systematic overview of the exciting and rapidly moving field of RNA biology. For the secondedition of Molecular Biology of RNA more introductory material has been incorporated at the beginning of the text, while new material has been included throughout - particularly in relation to RNA bindingdomains, non-coding RNAs, and the connection between RNA biology and epigenetics. Finally, a new closing chapter discusses how exciting new technologies are being used to explore current topical areas of research.


Biophysics of RNA-Protein Interactions

Biophysics of RNA-Protein Interactions
Author: Chirlmin Joo
Publisher: Springer Nature
Total Pages: 249
Release: 2019-09-19
Genre: Science
ISBN: 1493997262

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RNA molecules play key roles in all aspects of cellular life, but to do so efficiently, they must work in synergism with proteins. This book addresses how proteins and RNA interact to carry out biological functions such as protein synthesis, regulation of gene expression, genome defense, liquid phase separation and more. The topics addressed in this volume will appeal to researchers in biophysics, biochemistry and structural biology. The book is a useful resource for anybody interested in elucidating the molecular mechanisms and discrete properties of RNA-protein complexes. Included are reviews of key systems such as microRNA and CRISPR/Cas that exemplify how RNA and proteins work together to perform their biological function. Also covered are techniques ranging from single molecule fluorescence and force spectroscopy to crystallography, cryo-EM microscopy, and kinetic modeling.


RNA Methodologies

RNA Methodologies
Author: Robert E. Farrell Jr.
Publisher: Elsevier
Total Pages: 962
Release: 2022-11-22
Genre: Science
ISBN: 0323916562

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RNA Methodologies: A Laboratory Guide for Isolation and Characterization, Sixth Edition provides the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on RNA sequencing, RNA in Situ Hybridization, non-coding RNAs, computational RNA biology, transcriptomes and bioinformatics, along with the latest advances in methods and protocols across the field of RNA investigation. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA biology while also giving readers helpful hints and troubleshooting techniques from his own personal experience in this subject area. This book presents the essential knowledge and techniques to use when working with RNA for the experienced practitioner, while also aiding the beginner in fully understanding this important branch of molecular biology. Presents the latest information covering all aspects of working with RNA, delivering a holistic understanding of this leading field in molecular biology Builds from basic information on RNA techniques to in-depth protocols for specific applications Features new chapters on RNA sequencing and RNA in situ hybridization Includes new material on RNA clinical applications and innovations, including RNA therapeutics and RNA vaccines, with particular relevance to coronavirus Comprises the latest developments in transcriptomes and bioinformatics, with new material on computational RNA biology, RNA CHiP analysis, aptamer biology and RNA epigenetics


RNA

RNA
Author: Dieter Söll
Publisher: Pergamon Press
Total Pages: 342
Release: 2001
Genre: Science
ISBN: 0080434088

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This publication summarizes the current status of our understanding of RNA, with particular emphasis on the chemistry of this key biological molecule. The various RNAs covered are messenger RNA, ribosomal RNA, transfer RNA and RNA enzymes (ribozymes). The different chapters detail biophysical and chemical methods to investigate RNA structure and function, the synthesis of native and modified RNAs and the latest advances in our understanding of the vast array of biological processes in which RNA is involved.