Quantitative Techniques for Imaging Cells and Tissues
| Author | : |
| Publisher | : |
| Total Pages | : |
| Release | : 2015 |
| Genre | : |
| ISBN | : |
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Quantitative Techniques For Imaging Cells And Tissues PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Quantitative Techniques For Imaging Cells And Tissues PDF full book. Access full book title Quantitative Techniques For Imaging Cells And Tissues.
Quantitative Imaging in Cell Biology
| Author | : |
| Publisher | : Academic Press |
| Total Pages | : 609 |
| Release | : 2014-06-25 |
| Genre | : Science |
| ISBN | : 0124202012 |
Download Quantitative Imaging in Cell Biology Book in PDF, ePub and Kindle
This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material
Quantitative Phase Imaging of Cells and Tissues
| Author | : Gabriel Popescu |
| Publisher | : McGraw Hill Professional |
| Total Pages | : 384 |
| Release | : 2011-03-14 |
| Genre | : Technology & Engineering |
| ISBN | : 0071663436 |
Download Quantitative Phase Imaging of Cells and Tissues Book in PDF, ePub and Kindle
Cutting-edge quantitative phase imaging techniques and their applications Filled with unique, full-color images taken by advanced quantitative phase imaging (QPI), Quantitative Phase Imaging of Cells and Tissues thoroughly explores this innovative technology and its biomedical applications. An introductory background on optical imaging and traditional optical microscopy is included to illustrate concept development. The book explains how various visualization modalities can be obtained by numerical calculations. This authoritative resource reveals how to take full advantage of the unprecedented capabilities of QPI, such as rendering scattering properties of minute subcellular structures and nanoscale fluctuations in live cells. Coverage includes: Groundwork Spatiotemporal field correlations Image characteristics Light microscopy Holography Point scanning QPI methods Principles of full-field QPI Off-axis full-field methods Phase-shifting techniques Common-path methods White light techniques Fourier transform light scattering (FTLS) Current trends in QPI
Cell Imaging Techniques
| Author | : Douglas J. Taatjes |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 505 |
| Release | : 2008-02-04 |
| Genre | : Medical |
| ISBN | : 1592599931 |
Download Cell Imaging Techniques Book in PDF, ePub and Kindle
A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.
Advanced Imaging and Bio Techniques for Convergence Science
| Author | : Jun Ki Kim |
| Publisher | : Springer Nature |
| Total Pages | : 560 |
| Release | : 2021-04-08 |
| Genre | : Medical |
| ISBN | : 981336064X |
Download Advanced Imaging and Bio Techniques for Convergence Science Book in PDF, ePub and Kindle
This book is a wide-ranging guide to advanced imaging techniques and related methods with important applications in translational research or convergence science as progress is made toward a new era in integrative healthcare. Conventional and advanced microscopic imaging techniques, including both non-fluorescent (i.e., label-free) and fluorescent methods, have to date provided researchers with specific and quantitative information about molecules, cells, and tissues. Now, however, the different imaging techniques can be correlated with each other and multimodal methods developed to simultaneously obtain diverse and complementary information. In addition, the latest advanced imaging techniques can be integrated with non-imaging techniques such as mass spectroscopic methods, genome editing, organic/inorganic probe synthesis, nanomedicine, and drug discovery. The book will be of high value for researchers in the biological and biomedical sciences or convergence science who need to use these multidisciplinary and integrated techniques or are involved in developing new analytical methods focused on convergence science.
Live Cell Imaging
| Author | : Robert D. Goldman |
| Publisher | : CSHL Press |
| Total Pages | : 658 |
| Release | : 2005 |
| Genre | : Science |
| ISBN | : 9780879696832 |
Download Live Cell Imaging Book in PDF, ePub and Kindle
Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.
Stimulated Raman Scattering Microscopy
| Author | : Ji-Xin Cheng |
| Publisher | : Elsevier |
| Total Pages | : 612 |
| Release | : 2021-12-04 |
| Genre | : Science |
| ISBN | : 0323903371 |
Download Stimulated Raman Scattering Microscopy Book in PDF, ePub and Kindle
Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics
Multi-Parametric Live Cell Microscopy of 3D Tissue Models
| Author | : Ruslan I. Dmitriev |
| Publisher | : Springer |
| Total Pages | : 172 |
| Release | : 2017-10-26 |
| Genre | : Science |
| ISBN | : 3319673580 |
Download Multi-Parametric Live Cell Microscopy of 3D Tissue Models Book in PDF, ePub and Kindle
This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.
Quantitative Phase Imaging
| Author | : Gabriel Popescu |
| Publisher | : |
| Total Pages | : |
| Release | : 2015 |
| Genre | : Diagnostic imaging |
| ISBN | : |
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'Proceedings of SPIE' presents the original research papers presented at SPIE conferences and other high-quality conferences in the broad-ranging fields of optics and photonics. These books provide prompt access to the latest innovations in research and technology in their respective fields.
Web Microanalysis of Big Image Data
| Author | : Peter Bajcsy |
| Publisher | : Springer |
| Total Pages | : 211 |
| Release | : 2018-01-22 |
| Genre | : Technology & Engineering |
| ISBN | : 3319633600 |
Download Web Microanalysis of Big Image Data Book in PDF, ePub and Kindle
This book looks at the increasing interest in running microscopy processing algorithms on big image data by presenting the theoretical and architectural underpinnings of a web image processing pipeline (WIPP). Software-based methods and infrastructure components for processing big data microscopy experiments are presented to demonstrate how information processing of repetitive, laborious and tedious analysis can be automated with a user-friendly system. Interactions of web system components and their impact on computational scalability, provenance information gathering, interactive display, and computing are explained in a top-down presentation of technical details. Web Microanalysis of Big Image Data includes descriptions of WIPP functionalities, use cases, and components of the web software system (web server and client architecture, algorithms, and hardware-software dependencies). The book comes with test image collections and a web software system to increase the reader's understanding and to provide practical tools for conducting big image experiments. By providing educational materials and software tools at the intersection of microscopy image analyses and computational science, graduate students, postdoctoral students, and scientists will benefit from the practical experiences, as well as theoretical insights. Furthermore, the book provides software and test data, empowering students and scientists with tools to make discoveries with higher statistical significance. Once they become familiar with the web image processing components, they can extend and re-purpose the existing software to new types of analyses. Each chapter follows a top-down presentation, starting with a short introduction and a classification of related methods. Next, a description of the specific method used in accompanying software is presented. For several topics, examples of how the specific method is applied to a dataset (parameters, RAM requirements, CPU efficiency) are shown. Some tips are provided as practical suggestions to improve accuracy or computational performance.
