Photoproteins from Jellyfish Aequorea Victoria
Author | : Emre Dikici |
Publisher | : |
Total Pages | : 340 |
Release | : 2008 |
Genre | : |
ISBN | : |
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Author | : Emre Dikici |
Publisher | : |
Total Pages | : 340 |
Release | : 2008 |
Genre | : |
ISBN | : |
Author | : Sapna K. Deo |
Publisher | : |
Total Pages | : 338 |
Release | : 2000 |
Genre | : |
ISBN | : |
Author | : Sylvia Daunert |
Publisher | : John Wiley & Sons |
Total Pages | : 256 |
Release | : 2006-12-13 |
Genre | : Science |
ISBN | : 3527608699 |
The use of light-emitting proteins for the detection of biomolecules provides fast and sensitive methods which overcome the disadvantages of radioactive labels and the high cost of fluorescent dyes. This reference work summarizes modern advanced techniques and their applications and includes practical examples of assays based on photoproteins. The book presents contemporary key topics like luminescent marine organisms, DNA probes, reporter gene assays and photoproteins, ratiometric sensing, use of photoproteins for in vivo functional imaging and luminescent proteins in binding assays, to name just a few, and is complemented by recent advances in instrumentation. Includes an introductory chapter by 2008 Chemistry Nobel laureate Osamu Shimomura.
Author | : Sylvia Daunert |
Publisher | : Wiley-VCH |
Total Pages | : 256 |
Release | : 2006-12-13 |
Genre | : Science |
ISBN | : 3527608699 |
The use of light-emitting proteins for the detection of biomolecules provides fast and sensitive methods which overcome the disadvantages of radioactive labels and the high cost of fluorescent dyes. This reference work summarizes modern advanced techniques and their applications and includes practical examples of assays based on photoproteins. The book presents contemporary key topics like luminescent marine organisms, DNA probes, reporter gene assays and photoproteins, ratiometric sensing, use of photoproteins for in vivo functional imaging and luminescent proteins in binding assays, to name just a few, and is complemented by recent advances in instrumentation. Includes an introductory chapter by 2008 Chemistry Nobel laureate Osamu Shimomura.
Author | : Shimomura Osamu |
Publisher | : World Scientific |
Total Pages | : 556 |
Release | : 2019-06-06 |
Genre | : Science |
ISBN | : 9813277122 |
This book is the bible of bioluminescence and a must-read not only for the students but for those who work in various fields relating to bioluminescence. It summarizes current structural information on all known bioluminescent systems in nature, from well-studied ones to those that have been seldom investigated.This book remains an important source of chemical knowledge on bioluminescence and, since the second edition's publication in 2012, has been revised to include major developments in two systems: earthworm Fridericia and higher fungi whose luciferins have been elucidated and synthesized. These two new luciferins represent an essential addition to seven previously known, with fully rewritten sections covering this new subject matter.
Author | : Leonid L. Moroz |
Publisher | : Springer Nature |
Total Pages | : 588 |
Release | : |
Genre | : |
ISBN | : 1071636421 |
Author | : Richard Nuccitelli |
Publisher | : Academic Press |
Total Pages | : 418 |
Release | : 1994-03-10 |
Genre | : Medical |
ISBN | : 9780125641418 |
A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation. Describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodes Fluorescent dyes Luminescent proteins Includes techniques for perturbing intracellular Ca2+ Covers detailed methodology plus problems and pitfalls of each technique Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program Color plates illustrate techniques such as Confocal ratio-imaging Use of aequorin
Author | : Jörg Hüser |
Publisher | : John Wiley & Sons |
Total Pages | : 362 |
Release | : 2006-12-13 |
Genre | : Science |
ISBN | : 3527609369 |
Backed by leading authorities, this is a professional guide to successful compound screening in pharmaceutical research and chemical biology, including the chemoinformatic tools needed for correct data evaluation. Chapter authors from leading pharmaceutical companies as well as from Harvard University discuss such factors as chemical genetics, binding, cell-based and biochemical assays, the efficient use of compound libraries and data mining using cell-based assay results. For both academics and professionals in the pharma and biotech industries working on small molecule screening.
Author | : Martin Chalfie |
Publisher | : John Wiley & Sons |
Total Pages | : 486 |
Release | : 2005-11-18 |
Genre | : Science |
ISBN | : 0471739480 |
Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications. This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.
Author | : Sascha Peters |
Publisher | : Walter de Gruyter |
Total Pages | : 227 |
Release | : 2014-02-24 |
Genre | : Design |
ISBN | : 3038210021 |
Following the huge success of Material Revolution, this second volume addresses the rapid development of material research and presents materials new to the market since 2010. The significance of sustainable and intelligent materials in design and architecture has increased enormously over the last two years. Numerous new products have been introduced to the market and designers’ thirst for knowledge about the sustainability of new material is as strong as ever, making a sequel to Material Revolution necessary. The new volume contains a similar system of classification but covers a completely different range of materials. There is a chapter dedicated solely to the criteria and factors of sustainable product design, as well as to innovative projects by designers and architects that work with new materials and technologies.